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Species: Xenopus laevis
Genetic Insert: VSP1 R152Q
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21618529
Proper citation: RRID:Addgene_51881 Copy
Species: Xenopus laevis
Genetic Insert: VSP1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21618529
Proper citation: RRID:Addgene_51883 Copy
Species: Xenopus laevis
Genetic Insert: VSP1 C301S
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21618529
Proper citation: RRID:Addgene_51882 Copy
Species: Xenopus laevis
Genetic Insert: Intersectin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47407 Copy
Species: Xenopus laevis
Genetic Insert: Engrailed-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Xenopus Probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1679005
Comments: In situ probe cut with Xba1, transcribe with T3.
Proper citation: RRID:Addgene_16950 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-5A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8275867
Comments: Insert: cDNA from Melton lambda gt10 oocyte library.
Transcription: linearize with Xba1 and transcribe with SP6.
Plasmid History:
Constructed by Randall Moon on date of 9/15/90.
Notes for Use:
EcoR1 insert of lambda G6 of 2/11/90. Contains significant 5' UTR and is less translatable in vitro per ng RNA than Xwnt-5A Hind III/SP64T. However, the type of activity is the same in both constructs.
Citation: Moon et al., Development 119, 97 (1993)
Proper citation: RRID:Addgene_16985 Copy
Species: Xenopus laevis
Genetic Insert: banded hedgehog
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Xenopus Hedgehog. Antisense are generated as listed:
Xenopus banded hedgehog: Linearize clone XIL in Bluescript KS with BamH1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus sonic hedgehog: Linearize clone X32 in Bluescript KS with Bam H1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus cephalic hedgehog:Linearize clone X30 in pT7TS with Sal 1 and transcribe with T7.
Proper citation: RRID:Addgene_16984 Copy
Species: Xenopus laevis
Genetic Insert: Goosecoid
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: coding region of Xenopus goosecoid in SP64T in sense (#9) & antisense (#2) orientation.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian by PCR.
Goosecoid cloned and characterized by lab of E. DeRobertis, UCLA.
Proper citation: RRID:Addgene_16979 Copy
Species: Xenopus laevis
Genetic Insert: Xbra
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:psp73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: full length Xbra from Jim Smith.
Antisense: linearize with EcoRV, transcribe with T7.
Plasmid History:
Constructed by Jan Christian.
Proper citation: RRID:Addgene_16978 Copy
Species: Xenopus laevis
Genetic Insert: noggin PCR clone
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Insert: noggin PCR clone (coding region). In reverse orientation compared to CSKA promoter.
Plasmid History:
Constructed by Jan Christian.
Isolated and characterize by W. Smith and R. Harland.
Proper citation: RRID:Addgene_16998 Copy
Species: Xenopus laevis
Genetic Insert: noggin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Insert: noggin PCR clone (coding region) in SP64T.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian.
Noggin isolated and chacterized by W. Smith and R. Harland.
Proper citation: RRID:Addgene_16997 Copy
Species: Xenopus laevis
Genetic Insert: Xbhh-C
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7671801
Comments: Expression ConstructXenopus Banded Hedgehog.
(a.k.a. Xbhh; hh1)
Insert: PCR of coding region, Genbank accession number U26404.
Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3.
Sense RNA: Linearize with Bam H1 and transcribe with T7.
References:
Ekker et al., Development 121, 2337-2347 (1995)
Lai et al., Development 121, 2349-2360 (1995)
Subclone Xbhh-N in pT7Ts.
Encodes amino terminal product with autoproteolytic cleavage. Linearize with BamH1 and transcribe with T7.
Subclone Xbhh-dominant negative-C in pT7TS:
Encodes a leader sequence, a few amino acids amino terminal to the autoproteolytic site, and the carboxy-terminal domain. Linearize with BamH1 and transcribe with T7.
Proper citation: RRID:Addgene_16995 Copy
Species: Xenopus laevis
Genetic Insert: Xbhh
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7671801
Comments: Expression Construct Xenopus Banded Hedgehog (a.k.a. Xbhh; hh1)
Insert: PCR of coding region, Genbank accession number U26404.
Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3.
Sense RNA: Linearize with Bam H1 and transcribe with T7.
References:
Ekker et al., Development 121, 2337-2347 (1995)
Lai et al., Development 121, 2349-2360 (1995)
Proper citation: RRID:Addgene_16993 Copy
Species: Xenopus laevis
Genetic Insert: C-cadherin
Vector Backbone Description: Backbone Size:0; Vector Backbone:pSP36T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7556920
Comments: Linearize with EcoRI and transcribe with SP6.
A cDNA encoding Xenopus C-cadherin was cloned by screening a Xenopus lambda gt10 oocyte cDNA library. The cDNA was instered into pSP36T for transcription using the SP6 promoter.
Proper citation: RRID:Addgene_17023 Copy
Species: Xenopus laevis
Genetic Insert: dominant N-cadherin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Full-length N-cadherin was digested with BamHI to remove nt 90-2004, deleting the extracellular domain while
keeping the signal sequence (identical to preparation by C. Kintner, 1992, Cell 69:225-236, who provided the cadherin).
Linearize and transcribe with T7.
Proper citation: RRID:Addgene_17025 Copy
Species: Xenopus laevis
Genetic Insert: beta-globin 5' UTR
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCD-PS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Description: This is an old but serviceable SV40 expression construct (pCD-PS) modified to give the Xenopus beta-globin untranslated regions and multiple cloning sites from pT7TS vector (Addgene plasmid 17091).
Reference: The vector was made by Julia Yang-Snyder.
Proper citation: RRID:Addgene_17120 Copy
Species: Xenopus laevis
Genetic Insert: Ecd8
Vector Backbone Description: Backbone Marker:R. Freeman/ S. Sokol; Backbone Size:3000; Vector Backbone:pXT7; Vector Types:Xenopus RNA expression ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10485853
Comments: Digest with Pst1, transcribe with T7 pol for sense RNA.
Proper citation: RRID:Addgene_32992 Copy
Species: Xenopus laevis
Genetic Insert: Xfz8
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9651509
Proper citation: RRID:Addgene_32990 Copy
Species: Xenopus laevis
Genetic Insert: HIPK2
Vector Backbone Description: Backbone Marker:Sergei Sokol; Vector Backbone:pCTX-myc; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20951344
Proper citation: RRID:Addgene_32996 Copy
Species: Xenopus laevis
Genetic Insert: XTCF3
Vector Backbone Description: Backbone Size:4097; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20951344
Comments: See author's map for complete restriction site details.
Proper citation: RRID:Addgene_32998 Copy
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