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Species: Other
Genetic Insert: DDdCas9VP192-P300-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-CAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102887 Copy
Species: Other
Genetic Insert: DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-CAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102888 Copy
Species: Other
Genetic Insert: DDdCas9VP192-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-tight; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102889 Copy
Species: Other
Genetic Insert: DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-CAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102886 Copy
Species: Other
Genetic Insert: mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
Vector Backbone Description: Vector Backbone:pSAM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
References:
Comments: The Plac promoter from pGFP-Mut3.1 (Clonetech), along with its associated ribosome binding sequence, was amplified via PCR. Engineered 59 BamHI and 39 NdeI restriction sites were used to sub-clone the resulting fragment into a BamHI/NdeI (New England Biolabs) double digested pSAM_Bt vector upstream of the himar1C9 transposase gene. The kanamycin resistance gene from pKD4 was amplified and ligated using the restriction sites MfeI and XbaI, replacing the erythromycin resistance gene ermG in pSAM_Bt. The resulting transposon mutagenesis vector, pSAM-Ec, was stored and propagated in the pir+ E. coli strain EcS17.
Proper citation: RRID:Addgene_102939 Copy
Species: Other
Genetic Insert: DDdCas9VP192-P300-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-tight; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102891 Copy
Species: Other
Genetic Insert: OCT4-gRNAs-1-5-PGK-puro
Vector Backbone Description: Vector Backbone:PB-GG-PGK-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102893 Copy
Species: Other
Genetic Insert: dCas9VPH-T2A-GFP-shP53
Vector Backbone Description: Vector Backbone:pCXLE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102895 Copy
Species: Other
Genetic Insert: dCas9VPP300-T2A-GFP-shP53
Vector Backbone Description: Vector Backbone:pCXLE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102896 Copy
Species: Other
Genetic Insert: dCas9VPPH-T2A-GFP-shP53
Vector Backbone Description: Vector Backbone:pCXLE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102897 Copy
Species: Other
Genetic Insert: dCas9GFP-shP53
Vector Backbone Description: Vector Backbone:pCXLE; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102906 Copy
Species: Other
Genetic Insert: OSK2M2L1-gRNAs-PGK-puro
Vector Backbone Description: Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102902 Copy
Species: Other
Genetic Insert: EEA-guide1-PGK-Puro
Vector Backbone Description: Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_102904 Copy
Species: Other
Genetic Insert: K2FTY8(Cas9 coding gene from Alcanivorax pacificus W11-5)
Vector Backbone Description: Backbone Marker:NEB(New England Biolabs); Vector Backbone:pUC19; Vector Types:CRISPR, Cloning vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103079 Copy
Species: Other
Genetic Insert: D3NT09(Cas9 coding gene from Azospirillum sp. (strain B510))
Vector Backbone Description: Backbone Marker:NEB(New England Biolabs); Vector Backbone:pUC19; Vector Types:CRISPR, Cloning vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103074 Copy
Species: Other
Genetic Insert: E6KTN8(Cas9 coding gene from Actinomyces sp. oral taxon 180 str. F0310)
Vector Backbone Description: Backbone Marker:NEB(New England Biolabs); Vector Backbone:pUC19; Vector Types:CRISPR, Cloning vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103076 Copy
Species: Other
Genetic Insert: E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
Vector Backbone Description: Backbone Marker:NEB(New England Biolabs); Vector Backbone:pUC19; Vector Types:CRISPR, Cloning vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103075 Copy
Species: Other
Genetic Insert: GFP-OVA
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: A short ovalbumin epitope (amino acids 319–343) was fused to GFP (pGFP_OVA) to follow the immune response to the expressed antigen. (Bumann, 2001).
Proper citation: RRID:Addgene_103059 Copy
Species: Other
Genetic Insert: B9M9X8(Cas9 coding gene from Acidovorax ebreus (strain TPSY))
Vector Backbone Description: Backbone Marker:NEB(New England Biolabs); Vector Backbone:pUC19; Vector Types:CRISPR, Cloning vector; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103072 Copy
Species: Other
Genetic Insert: TAL-L1-2.4-VP64
Vector Backbone Description: Vector Backbone:pRN3p; Vector Types:T3 in vitro transcription for expression in mammalian cells; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_103039 Copy
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