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Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26658467
Proper citation: RRID:Addgene_66936 Copy
Genetic Insert: traJ, cat, oriT
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Red Recombinase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28352570
Comments: Conjugally transferable red recombinase vector pMJH46
Proper citation: RRID:Addgene_67272 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: 8xHis-mCherry-attR1-ccdB-CamR-attR2-Stop
Proper citation: RRID:Addgene_67139 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: 8xHis-eGFP-attR1-ccdB-CamR-attR2-Stop
Proper citation: RRID:Addgene_67137 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: 8xHis-attR1-ccdB-CamR-attR2-Stop
Proper citation: RRID:Addgene_67135 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: attR1-ccdB-CamR-attR2-8xHis-Stop
Proper citation: RRID:Addgene_67136 Copy
Vector Backbone Description: Vector Backbone:pCellFree_G05; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: sfGFP
Proper citation: RRID:Addgene_67143 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: attR1-ccdB-CamR-attR2-mCheery-8xHis-Stop
Proper citation: RRID:Addgene_67140 Copy
Vector Backbone Description: Vector Backbone:pLTE EGFP; Vector Types:Cell-free expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25529348
Comments: Myc-8xHis-mCherry-attR1-ccdB-CamR-attR2-Stop
Proper citation: RRID:Addgene_67141 Copy
Vector Backbone Description: Vector Backbone:pSGE_240_pAGW_V5; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_71188 Copy
Vector Backbone Description: Vector Backbone:pSW_1; Vector Types:Gateway vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26550828
Proper citation: RRID:Addgene_71279 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71499 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71503 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71504 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Comments: This plasmid was made by replacing the sequence of pGL3-Promoter between BglII and FseI with a fragment containing the core promoter listed above, an mhc16 intron, an ORF (sgGFP, Qbiogene, Inc), a ccdB suicide gene flanked by homology arms (used for cloning the enhancer candidates during library generation), followed by the pGL3's SV40 late polyA-signal.
Proper citation: RRID:Addgene_71502 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Proper citation: RRID:Addgene_71507 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL3-Promoter; Vector Types:Insect Expression, flySTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25517091
Proper citation: RRID:Addgene_71508 Copy
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Comments: Note: The plasmid for enhancer-activity testing by luciferase assays has been used in the Arnold et al. 2013 publication. It should be replaced for all enhancer activity tests mammalian cells with the updated version reported by Muerdter et al. 2017. The improved version is Addgene plasmid # 99297. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017).
Gateway-cassette was added between the KpnI and BglII sites and SCP1 between BglII and HindIII
Proper citation: RRID:Addgene_71510 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBlueScript SK(+); Vector Types:Gateway entry vector for use in multisite Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21343429
Proper citation: RRID:Addgene_71744 Copy
Species: Mus musculus
Genetic Insert: Rosa26 5-homology region
Vector Backbone Description: Vector Backbone:Bluescript; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26772810
Proper citation: RRID:Addgene_74281 Copy
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