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Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33731932
Comments: pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)
Proper citation: RRID:Addgene_166828 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG5 ATG12 ATG16
Vector Backbone Description: Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26812546
Proper citation: RRID:Addgene_166851 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166855 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166854 Copy
Species: Xenopus laevis
Genetic Insert: Sizzled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9428410
Comments: This encodes a secreted
antagonist of Xwnt-8.
The entire open reading frame of sizzled was amplified by low-cycle number PCR using Pfu polymerase and cloned as a
BamHI-XhoI fragment into pCS2+ .
Proper citation: RRID:Addgene_16688 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Atg13[571–700]
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31352862
Proper citation: RRID:Addgene_166852 Copy
Species: Xenopus laevis
Genetic Insert: APC
Vector Backbone Description: Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9015311
Comments: Insert: Full length Xenopus APC with an amino terminal c-myc epitope in the vector pCS2+MT.
Insert size: ~8.5kb. NcoI site introduced at 5' end. Linearize with Not1 and transcribe with SP6.
Proper citation: RRID:Addgene_16686 Copy
Genetic Insert: csgBACEFG, with α-amylase fused to CsgA via flexible linker
Vector Backbone Description: Backbone Size:3763; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34141524
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.11.23.394965v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_166859 Copy
Genetic Insert: csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
Vector Backbone Description: Backbone Size:3763; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34141524
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.11.23.394965v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_166858 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166840 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166844 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VAC8
Vector Backbone Description: Vector Backbone:pRS405; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508216
Comments: Linearize with AflII
Proper citation: RRID:Addgene_166841 Copy
Species: Homo sapiens
Genetic Insert: Dkk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11448771
Comments: This is wild-type, full-length Dickkopf-1, Flag tagged.
Proper citation: RRID:Addgene_16690 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG1
Vector Backbone Description: Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30527663
Comments: Linearize with StuI
Proper citation: RRID:Addgene_166847 Copy
Species: Synthetic
Genetic Insert: U6-VEGFA array presgRNA
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:3315; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166873 Copy
Species: Synthetic
Genetic Insert: U6-VEGFA presgRNA
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:3316; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166872 Copy
Species: Synthetic
Genetic Insert: U6-control presgRNA
Vector Backbone Description: Backbone Marker:Cell Biolabs, Inc.; Backbone Size:3316; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34178991
Proper citation: RRID:Addgene_166871 Copy
Species: Synthetic
Genetic Insert: SARS-COV-2 Nsp8
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34154407
Proper citation: RRID:Addgene_166861 Copy
Species: Synthetic
Genetic Insert: SARS-COV-2 Nsp7
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34154407
Proper citation: RRID:Addgene_166860 Copy
Species: Synthetic
Genetic Insert: Phi29 Polymerase codon-optimized for overexpression in E. coli
Vector Backbone Description: Backbone Marker:GenScript; Vector Backbone:pGS-21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21071419
Proper citation: RRID:Addgene_166863 Copy
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