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Species: Homo sapiens
Genetic Insert: BUB1 S492Y
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:5099; Vector Backbone:pBI-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9521327
Comments: pBI-GFP-Bub1 S492Y was constructed by cloning RT-PCR products from tumour line V429 into the NotI and SalI sites of pBI-GFP.
Proper citation: RRID:Addgene_16625 Copy
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:5099; Vector Backbone:pBI-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9521327
Comments: Expression vector pBI-GFP was constructed by insertion of the blunt-ended HindIII/NotI GFP fragment of phGFP-S65T (Clontech) into the EcoRV site of pBI (Clontech).
Proper citation: RRID:Addgene_16623 Copy
Species: Homo sapiens
Genetic Insert: PTPRT R632X
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15155950
Comments: A 6kb fragment of PTPRT including the entire ORF and the 3'UTR was excised from KIAA clone (ha06139s1) with SgrAI and ScaI and cloned into the XmaI/EcoRV sites of pIRES2hyg2. A 1.9kb fragment containing PTPRT was digested with NheI and NotI and cloned into the pCI-Neo expression vector.
Proper citation: RRID:Addgene_16629 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA H1047R
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMV2-Tag 2A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15016963
Comments: This plasmid has 5 additional mutations in PIK3CA compared to NP_006209.2: N114D, L177S, T322A, A400V, L404P. These mutations do not alter the function of the encoded proteins.
Proper citation: RRID:Addgene_16639 Copy
Species: Homo sapiens
Genetic Insert: PTPRT
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15155950
Comments: A 6kb fragment of PTPRT including the entire ORF and the 3'UTR was excised from KIAA clone (ha06139s1) with SgrAI and ScaI and cloned into the XmaI/EcoRV sites of pIRES2hyg2. The fragment containing PTPRT was digested with NheI and NotI and cloned into the pCI-Neo expression vector.
Proper citation: RRID:Addgene_16630 Copy
Species: Other
Genetic Insert: 40XUAS , Luciferase
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166455 Copy
Species: Homo sapiens
Genetic Insert: mEGFP, YAPS127A-deltaCC
Vector Backbone Description: Vector Backbone:mEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166459 Copy
Species: Homo sapiens
Genetic Insert: mCherry, TEAD4-YBD(217-434)
Vector Backbone Description: Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166450 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5200; Vector Backbone:pIRESpuro2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11581659
Comments: This is an improved version of the vector used in the publication.
Note that although the SnapGene software recognizes the fluorescent protein as EYFP, this variant does not have the mutations required to convert GFP to YFP, and so should function more similarly to GFP.
Proper citation: RRID:Addgene_16616 Copy
Species: Homo sapiens
Genetic Insert: mCherry, TEAD4-IDR
Vector Backbone Description: Vector Backbone:pet22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166442 Copy
Species: Homo sapiens
Genetic Insert: FDXR knockout
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:3838; Vector Backbone:pFredA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11590433
Comments: 4.2 kb 5' targeting element (up to +1527). See author's map for more information.
Proper citation: RRID:Addgene_16614 Copy
Species: Homo sapiens
Genetic Insert: Cry2, mCherry, YAP-C-IDR(266-504)
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166447 Copy
Species: Homo sapiens
Genetic Insert: Cry2, mCherry, YAP-N-IDR(1-164)
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166446 Copy
Species: Homo sapiens
Genetic Insert: YAP4LE
Vector Backbone Description: Vector Backbone:pet25b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166445 Copy
Species: Homo sapiens
Genetic Insert: Cry2, mCherry, TEAD4-IDR(89-230)
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33606996
Proper citation: RRID:Addgene_166449 Copy
Species: Homo sapiens
Genetic Insert: PPAR delta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11226285
Comments: See author's map for more information. PKO2: used to delete exon 4 of PPAR delta. 5' arm is 1.8 kb and 3' arm is 4.0 kb.
Proper citation: RRID:Addgene_16619 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166235 Copy
Genetic Insert: Reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
Vector Backbone Description: Backbone Size:5399; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33790271
Comments: The pre-print of this article is available on BioRxiv.org
doi: https://doi.org/10.1101/2021.01.06.425528
Proper citation: RRID:Addgene_166232 Copy
Species: Homo sapiens
Genetic Insert: single chain-Fv, scFv
Vector Backbone Description: Backbone Marker:DDD, Science for Life Laboratory; Vector Backbone:pHAT6; Vector Types:Bacterial Expression, Recombinant Antibody Expression; Bacterial Resistance:Kanamycin
Comments: Target NCBI gene ID: 23476
Proper citation: RRID:Addgene_166513 Copy
Species: Other
Genetic Insert: Ty tag fused to mNeon
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_166237 Copy
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