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Species: Other
Genetic Insert: GDH2 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Promoter inducible by xylose. Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125043 Copy
Species: Other
Genetic Insert: TEF1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Promoter inducible by inulin and xylose. Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125040 Copy
Species: Other
Genetic Insert: TSA1 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Promoter inducible by heat (37-42 degrees). Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125045 Copy
Species: Other
Genetic Insert: TDH3 promoter
Vector Backbone Description: Backbone Marker:Dueber lab, UC Berkeley; Backbone Size:1667; Vector Backbone:YTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31134195
Comments: Contains the appropriate YTK-compatible Golden Gate overhangs for a type 2 part Please note that the minor differences between the Addgene NGS results and depositor's annotated sequence do not affect the plasmid function.
Proper citation: RRID:Addgene_125048 Copy
Vector Backbone Description: Vector Backbone:lambda dv; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7883185
Comments: MCS: HindIII, PstI, SalI/AccI, BamHI, SmaI, EcoRI
Proper citation: RRID:Addgene_125141 Copy
Vector Backbone Description: Vector Backbone:lambda dv; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7883185
Comments: MCS: EcoRI, SstI, KpnI, SmaI, BamHI, XbaI, SalI/AccI, PstI, SphI, HindIII
Proper citation: RRID:Addgene_125142 Copy
Vector Backbone Description: Vector Backbone:lambda dv; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7883185
Comments: MCS: HindIII, SphI, PstI, SalI/AccI, XbaI, BamHI, SmaI, KpnI, SstI, EcoRI
Proper citation: RRID:Addgene_125143 Copy
Vector Backbone Description: Vector Backbone:lambda dv; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7883185
Comments: MCS: NruI, XhoI, ClaI, SphI, KpnI, SmaI, SstI, EcoRI, XbaI, HindIII, PstI, MluI, SalI/AccI, AatII, BamHI, EcoRV, NaeI
Proper citation: RRID:Addgene_125144 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 4 x upstream activating sequence (UAS)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3078; Vector Backbone:Unknown; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31092928
Proper citation: RRID:Addgene_125149 Copy
Vector Backbone Description: Backbone Size:6926; Vector Backbone:pCAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32508591
Comments: Intent is to introduce low doses of pCAG Cre to recombine a subset of cells, such that Halotag can act as an internal control to mScarlet for use in in utero electroporation or other related techniques. Genetic overexpression can be easily attached following the mScarlet through use of a mcs after the P2A.
Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_125138 Copy
Species: Rattus norvegicus
Genetic Insert: fRCaMP2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3703; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30651353
Comments: fRCaMP2 is the D396A variant of the jRCaMP1a gene cloned from the pGP-CMV vector (#61563) published by Dana et al. 2016.
Proper citation: RRID:Addgene_125246 Copy
Species: Synthetic
Genetic Insert: AktAR2-D
Vector Backbone Description: Backbone Marker:Addgene plasmid # 60523; Backbone Size:5661; Vector Backbone:pSBbi-Pur; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: Not membrane-targeted. Insert subcloned from Addgene #64932. This plasmid is almost identical to Addgene plasmid #125195 but has a single amino change in the target peptide.
Proper citation: RRID:Addgene_125196 Copy
Species: Tobacco etch virus
Genetic Insert: TEV_SH
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pTH24 based on pDEST42; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16150509
Comments: Please note that this plasmid may run a dimer (>13kb) or multimer.
Proper citation: RRID:Addgene_125194 Copy
Species: Synthetic
Genetic Insert: gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
Vector Backbone Description: Vector Backbone:pLX208; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30942168
Proper citation: RRID:Addgene_125233 Copy
Species: Synthetic
Genetic Insert: Lyn-AktAR2-D
Vector Backbone Description: Backbone Marker:Addgene plasmid # 60523; Backbone Size:5661; Vector Backbone:pSBbi-Pur; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28646116
Comments: Insert subcloned from Addgene plasmid #64932. Targeted to membrane by lipid raft-targeting peptide (GCIKSKRKD) derived from human kinase LYN.
Proper citation: RRID:Addgene_125198 Copy
Species: Synthetic
Genetic Insert: Lyn-AktAR2-EV
Vector Backbone Description: Backbone Marker:Addgene plasmid # 60523; Backbone Size:5661; Vector Backbone:pSBbi-Pur; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: Insert subcloned from Addgene plasmid #64932. Targeted to membrane by lipid raft-targeting peptide (GCIKSKRKD) derived from human kinase LYN.
Proper citation: RRID:Addgene_125199 Copy
Species: Homo sapiens
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:origene; Backbone Size:7600; Vector Backbone:pLenti-c-Myc-DDK-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25795775
Proper citation: RRID:Addgene_125238 Copy
Species: Synthetic
Genetic Insert: β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30942168
Proper citation: RRID:Addgene_125236 Copy
Species: Homo sapiens
Genetic Insert: stem cell Ascl2 reporter, 4 repeats
Vector Backbone Description: Backbone Size:6256; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29425513
Proper citation: RRID:Addgene_125239 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Backbone Marker:Available at Addgene (plasmid #1764); Backbone Size:5150; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16339315
Comments: To construct a hemagglutinin (HA)-tagged allele of p110 alpha, a cDNA clone of human PIK3CA (obtained from the FLEXGene Repository of the Harvard Institute of Proteomics [HIP]) was subcloned into pBabepuro by PCR with primers containing HA-epitope coding sequence with subsequent restriction and ligation.
Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val. It could be a SNP. No functional changes have been observed so far in signaling assays.
Proper citation: RRID:Addgene_12522 Copy
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