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Species: Synthetic
Genetic Insert: gltA2-udhA guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65819 Copy
Species: Synthetic
Genetic Insert: udhA guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65818 Copy
Species: Synthetic
Genetic Insert: zwf guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65825 Copy
Species: Synthetic
Genetic Insert: proD guide
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_65820 Copy
Genetic Insert: RhlR
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26315440
Comments: Integrated into the attP site
Proper citation: RRID:Addgene_65954 Copy
Species: Sulfolobus solfataricus P2
Genetic Insert: DNA polymerase sliding clamp 1
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2206; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20607777
Comments: We cloned the PCNA1 gene from the cell suspension of S. solfataricus P2 strain (ATCC35092).
Proper citation: RRID:Addgene_66125 Copy
Species: Other
Genetic Insert: STp
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5996; Vector Backbone:pBR325; Vector Types:cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:3284902
Comments: Contains the E. coli STp gene probe (157 bp) cloned on PstI linkers into pBR325 Heat stable enterotoxin gene (porcine)
Proper citation: RRID:Addgene_66149 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27084942
Proper citation: RRID:Addgene_69779 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27084942
Comments: Please note that parental strain (MC4100) is streptomycin-resistant.
Proper citation: RRID:Addgene_69786 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: FnCpf1 locus without Cas genes
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26422227
Proper citation: RRID:Addgene_69974 Copy
Species: Francisella tularensis subsp. novicida
Genetic Insert: FnCpf1 locus
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26422227
Proper citation: RRID:Addgene_69973 Copy
Genetic Insert: Lasso 1
Vector Backbone Description: Backbone Marker:Registry of Standard Biological Parts; Backbone Size:2060; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25967060
Comments: Please see genebank file for annotations.
Proper citation: RRID:Addgene_69935 Copy
Species: alicyclobacillus acidoterrestris
Genetic Insert: AacC2c1 locus
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26593719
Proper citation: RRID:Addgene_70166 Copy
Species: leptotrichia shahii
Genetic Insert: LshC2c2 full locus
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26593719
Proper citation: RRID:Addgene_70168 Copy
Species: Synthetic
Genetic Insert: amyEopt
Vector Backbone Description: Backbone Marker:Fagan and Fairweather 2009; Backbone Size:7216; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27333161
Comments: Please note: the depositing lab has determined that a particular IS element present in E. coli strains such as DH5alpha and its derivatives has a high propensity to insert into this plasmid. The depositing lab recommends isolating the plasmid DNA and transforming into a strain similar to MC1061.
Publication describing the backbone vector pRPF185 as well as the sequencing primers:
Clostridium difficile has two parallel and essential Sec secretion systems. Fagan RP, Fairweather NF. J Biol Chem. 2011 Aug 5;286(31):27483-93. doi: 10.1074/jbc.M111.263889. Epub 2011 Jun 9.
PMID: 21659510
Proper citation: RRID:Addgene_70188 Copy
Vector Backbone Description: Vector Backbone:see comments; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:8412673
Comments: MCS: EcoRV-BglII-XhoI-BssHII-SpeI-BsiWI-AvrII-AflII-BstBI and potentially other sites
sacB was derived from pUM24 (Ried, J. L., and A. Colimer. Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921). The polylinker came, in large part, from the plC20R polylinker (Marsh et al.. 1984: Gene 32: 481-485) and a polylinker region of plasmid pJRD184 (Heusterspreute et al.. 1985: Gene 37: 299-304).
The erythromycin-resistance gene came from Horinouchi, S., and Weisblum, B. (J. Bacteriol. 150: 804-814, 1982).
Proper citation: RRID:Addgene_70688 Copy
Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus dCas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GGGCACGGGCAGCTTGCCGG. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.
Proper citation: RRID:Addgene_70703 Copy
Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus Cas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GGGCACGGGCAGCTTGCCGG. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.
Proper citation: RRID:Addgene_70702 Copy
Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus Cas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GTCGCCCTCGAACTTCACCT. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.
Proper citation: RRID:Addgene_70705 Copy
Species: Synthetic
Genetic Insert: AD1 Sense
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:Custom; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25643173
Proper citation: RRID:Addgene_71207 Copy
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