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Species: Leptotrichia shahii
Genetic Insert: LsCas13a-gRNA-NT
Vector Backbone Description: Backbone Size:3267; Vector Backbone:pBAD18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Two repeats without a spacer sequence for use as negative /non-targeting control.
Proper citation: RRID:Addgene_164857 Copy
Species: Bergeyella zoohelcum
Genetic Insert: BzCas13b-gRNA-1
Vector Backbone Description: Backbone Size:4215; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164858 Copy
Species: Homo sapiens
Genetic Insert: TLN2
Vector Backbone Description: Vector Backbone:pcDNA4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164841 Copy
Species: Homo sapiens
Genetic Insert: TLN2
Vector Backbone Description: Vector Backbone:pcDNA4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164842 Copy
Species: Homo sapiens
Genetic Insert: TLN2
Vector Backbone Description: Vector Backbone:pcDNA4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164840 Copy
Species: Homo sapiens
Genetic Insert: VCL
Vector Backbone Description: Vector Backbone:pET3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164843 Copy
Species: Aedes aegypti
Genetic Insert: 4 gRNAs and 3xP3-tdTomato
Vector Backbone Description: Backbone Size:5260; Vector Backbone:piggyBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164848 Copy
Species: Other
Genetic Insert: hAPOBEC3A fused to PspCas13b
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164874 Copy
Species: Other
Genetic Insert: hAPOBEC3A inserted into RfxCas13d
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164873 Copy
Species: Homo sapiens
Genetic Insert: PIG3 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBR322pl-LUC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Pig3 promoter from -5602 to +537.
Proper citation: RRID:Addgene_16489 Copy
Species: Homo sapiens
Genetic Insert: TCF4 DNA binding domain
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16488 Copy
Species: Homo sapiens
Genetic Insert: APC
Vector Backbone Description: Backbone Size:0; Vector Backbone:pSAR-MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16487 Copy
Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET-33b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Full length DPC4/SMAD4 cDNA (codons 1-552)
Proper citation: RRID:Addgene_16491 Copy
Species: Synthetic
Genetic Insert: Enhanced green fluorescent protein
Vector Backbone Description: Backbone Size:7140; Vector Backbone:MSCVpic2; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164919 Copy
Species: Homo sapiens
Genetic Insert: PIG3 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBR322pl-LUC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Pig3 promoter from -862 to +537.
Proper citation: RRID:Addgene_16490 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: P(Z)-mcherry
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pNTI661; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164918 Copy
Species: Other
Genetic Insert: dcas13d-ADARdd
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164879 Copy
Species: Other
Genetic Insert: Split GFP(beta11)
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164876 Copy
Species: Other
Genetic Insert: BFP-preSTOP-GFP
Vector Backbone Description: Vector Backbone:puc57; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164877 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5024; Vector Backbone:pNTI661; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: See details in Muller et al for cloning barcoded gRNA libraries and ORFs of interest into this backbone.
Proper citation: RRID:Addgene_164915 Copy
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