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Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_205017 Copy
Species: Rattus norvegicus
Genetic Insert: cacnb1b
Vector Backbone Description: Backbone Marker:Invitrogen Life Technologies; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_206105 Copy
Species: Synthetic
Genetic Insert: PTtg-YFP
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37348465
Proper citation: RRID:Addgene_202300 Copy
Species: Paraburkholderia xenovorans
Genetic Insert: Encapsulin
Vector Backbone Description: Vector Backbone:pSH-EFIRES-P; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37932397
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197062 Copy
Species: Oryctolagus cuniculus
Genetic Insert: cacna1b
Vector Backbone Description: Backbone Marker:Invitrogen Life Technologies; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21233207
Comments: 5' end of DNA is very GC rich
Proper citation: RRID:Addgene_206071 Copy
Species: Synechococcus elongatus
Genetic Insert: Encapsulin
Vector Backbone Description: Vector Backbone:pSH-EFIRES-P; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37932397
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197061 Copy
Species: Rattus norvegicus
Genetic Insert: cacna2d1
Vector Backbone Description: Backbone Marker:Invitrogen Life Technologies; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35238638
Proper citation: RRID:Addgene_206070 Copy
Species: Rattus norvegicus
Genetic Insert: cacna1a
Vector Backbone Description: Backbone Marker:PMID 11397804; Backbone Size:4890; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11606638
Proper citation: RRID:Addgene_206076 Copy
Species: Synechococcus elongatus
Genetic Insert: I3-01
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37932397
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197060 Copy
Species: Synthetic
Genetic Insert: ameRS
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37348465
Proper citation: RRID:Addgene_202309 Copy
Species: S. pyogenes
Genetic Insert: B-Hypa2SpCas9
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37717069
Comments: pCbh-3xFLAG-NLS-Blackjack-Hypa2SpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations).
Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-Hypa2SpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, Hypa2SpCas9.
The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457).
For detailed information and plasmid usage, please see the associated publication. Please visit https://doi.org/10.1101/2022.08.16.504097 for bioRxiv preprint.
Proper citation: RRID:Addgene_126764 Copy
Species: Synechococcus elongatus
Genetic Insert: Encapsulin
Vector Backbone Description: Vector Backbone:pSH-EFIRES-B; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37932397
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197066 Copy
Genetic Insert: MitoTrap-mCherry
Vector Backbone Description: Vector Backbone:pSH-EFIRES-P; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37932397
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197067 Copy
Species: Epstein-Barr virus
Genetic Insert: BGLF5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195813 Copy
Species: Epstein-Barr virus
Genetic Insert: LF1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195812 Copy
Species: Epstein-Barr virus
Genetic Insert: BFRF3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195778 Copy
Species: Epstein-Barr virus
Genetic Insert: BGLF4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195811 Copy
Species: Epstein-Barr virus
Genetic Insert: LF2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195810 Copy
Species: Epstein-Barr virus
Genetic Insert: BFRF0.5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195772 Copy
Species: Epstein-Barr virus
Genetic Insert: BLLF2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2875; Vector Backbone:pENTR223.1; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17446270
Proper citation: RRID:Addgene_195774 Copy
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