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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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pCAGGS-mCherry Resource Report Resource Website 10+ mentions |
RRID:Addgene_41583 | Ampicillin | PMID:22546613 | Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:46:53 | 34 | ||||
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FLM-R4154C Resource Report Resource Website |
RRID:Addgene_41580 | PKD1 | Homo sapiens | Ampicillin | PMID:21518865 | Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | FLM R4136G | 2026-07-25 12:46:53 | 0 | |
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pFA6a-kanMX6-PGAL1-GFP Resource Report Resource Website |
RRID:Addgene_41614 | GAL1 promoter | Saccharomyces cerevisiae | Ampicillin | PMID:9717241 | Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin | 2026-07-25 12:46:54 | 0 | ||
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FLM-R4136G Resource Report Resource Website |
RRID:Addgene_41579 | PKD1 | Homo sapiens | Ampicillin | PMID:21518865 | Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | FLM R4136G | 2026-07-25 12:46:53 | 0 | |
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pFA6a-kanMX6-PGAL1-GST Resource Report Resource Website |
RRID:Addgene_41611 | GAL1 promoter | Saccharomyces cerevisiae | Ampicillin | PMID:9717241 | Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin | 2026-07-25 12:46:54 | 0 | ||
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pSH-hPXR-LBD-T248E Resource Report Resource Website |
RRID:Addgene_41619 | hPXR-LBD-T248E | Homo sapiens | Ampicillin | Human PXR ligand binding domain (LBD, aa 107-434) was cloned into vector pSH. Mutations were made and confirmed by sequencing. | Backbone Marker:ATCC (Item #77476); Backbone Size:5900; Vector Backbone:pSH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | contains human PXR ligand binding domain (LBD, aa 107-434), T248E | 2026-07-25 12:46:54 | 0 | |
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NTSP-PC1 Resource Report Resource Website |
RRID:Addgene_41575 | PKD1 | Homo sapiens | Ampicillin | PMID:21518865 | 5' cloning site: EcoR1 3' cloning site: XhoI | Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 4106-4218 of FLS PC1 | 2026-07-25 12:46:53 | 0 |
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4xCSL-luciferase Resource Report Resource Website 10+ mentions |
RRID:Addgene_41726 | 4xCSL binding sites | Ampicillin | PMID:11518718 | The 4xCSL-luciferase reporter was constructed from the CBF1/pGL2-GLO TATA CAT plasmid, a gift from Dr. S. Speck. A fragment containing the multimerized high affinity CSL sites (4× CGTGGGAA) was excised by BamHI digest and ligated into a BglII/BamHI-digested RSV-TATA pGL2 vector, a gift from Dr. D. Towler. This modified vector has a TATA box from Rous sarcoma virus inserted into the pGL2-basic vector (Promega) to reduce the basal luciferase activity. Construct was sequenced for verification. Alternate plasmid name: 4xCBS-luciferase | Backbone Marker:Dr. D. Towler (Washington University, St. Louis); Vector Backbone:RSV-TATA pGL2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | Contains a multimerized high affinity CSL binding site (4 x CGTGGGAA) | 2026-07-25 12:46:55 | 22 | |
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Hes5-Luc Resource Report Resource Website 1+ mentions |
RRID:Addgene_41724 | Hes5 Promoter | Mus musculus | Ampicillin | PMID:9570950 | Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | Contains the murine Hes5 promoter (-800 to +73) | 2026-07-25 12:46:55 | 7 | |
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pHes1(467)-luc Resource Report Resource Website 1+ mentions |
RRID:Addgene_41723 | Hes1 Promoter (-467 to +46) | Mus musculus | Ampicillin | PMID:9570950 | Alternate plasmid name: Hes1-Luc | Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | Contains the murine Hes1 promoter (-467 to +46) | 2026-07-25 12:46:55 | 8 |
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pSKDuet26 Resource Report Resource Website |
RRID:Addgene_41689 | Tvo VMA intein | Thermoplasma volcanium | Kanamycin | PMID:22001202 | Backbone Marker:novagen; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-07-25 12:46:54 | 0 | ||
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pSM1960 Resource Report Resource Website 1+ mentions |
RRID:Addgene_41842 | SEC63-mRFP | Saccharomyces cerevisiae | Ampicillin | PMID:18812321 | Please note that Addgene's quality control sequence shows several discrepancies with depositor's reference sequence. These are in vector region and should not affect function. | Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-07-25 12:46:55 | 2 | |
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pRP Resource Report Resource Website 1+ mentions |
RRID:Addgene_41841 | Ampicillin | PMID:23852599 | Backbone Marker:Invitrogen; Backbone Size:6577; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-07-25 12:46:55 | 3 | ||||
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pRLuc110UGA Resource Report Resource Website 1+ mentions |
RRID:Addgene_41729 | RLuc110UGA | Ampicillin | PMID:19208811 | Backbone Marker:Promega; Backbone Size:4079; Vector Backbone:pRL-CMV; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | in-frame UGA stop codon at codon 110 in the open reading frame of the RLuc cDNA | 2026-07-25 12:46:55 | 1 | ||
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pcDNA3-N-I-N-CAAX Resource Report Resource Website |
RRID:Addgene_41686 | Dronpa145N-IntersectinDH-Dronpa145N-CAAX | Homo sapiens | Ampicillin | PMID:23139335 | Backbone Size:5370; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | K145N on Dronpa | 2026-07-25 12:46:54 | 0 | |
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pRP_ASC-LmCerulean Resource Report Resource Website 1+ mentions |
RRID:Addgene_41840 | apoptosis-associated speck-like protein containing CARD | Homo sapiens | Ampicillin | PMID:23852599 | Backbone Size:7332; Vector Backbone:pRP_LmCerulean; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Stop Codon removed | 2026-07-25 12:46:55 | 6 | |
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pcDNA3.1(+)mGAT1-577-CFP-578CT Resource Report Resource Website 1+ mentions |
RRID:Addgene_41682 | Mus musculus GABA transporter 1 | Mus musculus | Ampicillin | PMID:19948998 | To generate the fluorescent mutants mGAT10XFP and mGAT1XFP* through mGAT1XFP45, the wild-type mGAT1 open reading frame (ORF) was subcloned without its original stop codon into the HindIII and EcoRI sites of the pcDNA3.1(+) expression vector multiple cloning site (MCS). XFP ORFs were then subcloned downstream from and in frame with the mGAT1 ORF at the NotI and XbaI sites of the pcDNA3.1(+) MCS. This resulted in a 12–amino acid spacer between the end of the mGAT1 sequence and the beginning of the fluorophore. The depositors modified a method for the integration of PCR fragments without the use of restriction enzymes (Geiser et al., 2001) to add the final 3, 8, 20, 28, or 45 codons of the human GAT1 (hGAT1) ORF. These were amplified from a source plasmid using the proof-reading PfuTurbo Cx Hotstart polymerase with 5′ and 3′ extensions corresponding to the 20–22-nt regions that flanked the intended site of insertion, such that the PCR product integrated in-frame immediately after the fluorophore sequence when used as the primers in a subsequent QuikChange II XL mutagenesis PCR reaction. For mGAT1XFP*, the depositors simply added a GTC codon for Val after the fluorophore ORF. The attached image displays the protein sequences of the modified regions of mGAT1 for each fluorescent construct. mGAT10CFP and mGAT10YFP repeated the fusion design of mGAT10GFP but with the fluorophore exchanged as annotated. The three C-terminal residues of the mGAT0XFP fusions are -YKI-CO2−, which comprises a broadly defined consensus PDZ class II–interacting motif (X-φ-X-φ, where φ designates a hydrophobic residue and X any residue) (Sheng and Sala, 2001; Hung and Sheng, 2002). The depositors searched the Ensembl databases using Biomart (http://www.ebi.ac.uk/biomart) (Spudich et al., 2007) and applied the GO:0005886 “plasma membrane” cellular component filter. The search identified no known membrane proteins possessing the -YKI-CO2− C-terminal sequence. In the mGAT1XFP* constructs, the depositors defined the terminal residue P(0) more narrowly, changing the terminal isoleucine residue present in mGAT10XFP to a valine in mGAT1XFP*. The resulting C-terminal sequence, -YKV-CO2−, reconstituted a functional PDZ class II–interacting motif present in Ephrin B receptors, a class that relies on interactions with the PDZ domain–containing proteins for clustering (Torres et al., 1998; Brückner et al., 1999; Lin et al., 1999; Madsen et al., 2005). Other constructs in the C-terminal XFP fusion series, mGAT1XFP3, mGAT1XFP8, mGAT1XFP20, mGAT1XFP28, and mGAT1XFP45, had the most C-terminal 3, 8, 20, 28, or 45 residues of the hGAT1 appended after the mGAT1XFP fusion. The differences in nucleotide sequence between the hGAT1 and mGAT1 C termini were a useful source of positive identification when the depositors analyzed the clones during construction. PCR integration was applied to amplify and insert EYFP or ECFP directly between residues R565 and L566, I570 and Q571, or V577 and R578 of mGAT1 to generate the mGAT15xxXFP5xxCT constructs. The site of XFP insertion in GAT1 is highlighted in the nomenclatures for these constructs by residue numbers flanking the fluorophore, and the “CT” denotes that the insertion occurs within the C terminus. Please see the associated article for more detailed information regarding construct creation and usage. | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | “Monomerizing” CFP A206K mutation; CFP inserted directly between residues V577 and R578 of mGAT1 | 2026-07-25 12:46:54 | 1 |
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pcDNA3.1(+)mGAT1-577-YFP-578CT Resource Report Resource Website 1+ mentions |
RRID:Addgene_41681 | Mus musculus GABA transporter 1 | Mus musculus | Ampicillin | PMID:19948998 | To generate the fluorescent mutants mGAT10XFP and mGAT1XFP* through mGAT1XFP45, the wild-type mGAT1 open reading frame (ORF) was subcloned without its original stop codon into the HindIII and EcoRI sites of the pcDNA3.1(+) expression vector multiple cloning site (MCS). XFP ORFs were then subcloned downstream from and in frame with the mGAT1 ORF at the NotI and XbaI sites of the pcDNA3.1(+) MCS. This resulted in a 12–amino acid spacer between the end of the mGAT1 sequence and the beginning of the fluorophore. The depositors modified a method for the integration of PCR fragments without the use of restriction enzymes (Geiser et al., 2001) to add the final 3, 8, 20, 28, or 45 codons of the human GAT1 (hGAT1) ORF. These were amplified from a source plasmid using the proof-reading PfuTurbo Cx Hotstart polymerase with 5′ and 3′ extensions corresponding to the 20–22-nt regions that flanked the intended site of insertion, such that the PCR product integrated in-frame immediately after the fluorophore sequence when used as the primers in a subsequent QuikChange II XL mutagenesis PCR reaction. For mGAT1XFP*, the depositors simply added a GTC codon for Val after the fluorophore ORF. The attached image displays the protein sequences of the modified regions of mGAT1 for each fluorescent construct. mGAT10CFP and mGAT10YFP repeated the fusion design of mGAT10GFP but with the fluorophore exchanged as annotated. The three C-terminal residues of the mGAT0XFP fusions are -YKI-CO2−, which comprises a broadly defined consensus PDZ class II–interacting motif (X-φ-X-φ, where φ designates a hydrophobic residue and X any residue) (Sheng and Sala, 2001; Hung and Sheng, 2002). The depositors searched the Ensembl databases using Biomart (http://www.ebi.ac.uk/biomart) (Spudich et al., 2007) and applied the GO:0005886 “plasma membrane” cellular component filter. The search identified no known membrane proteins possessing the -YKI-CO2− C-terminal sequence. In the mGAT1XFP* constructs, the depositors defined the terminal residue P(0) more narrowly, changing the terminal isoleucine residue present in mGAT10XFP to a valine in mGAT1XFP*. The resulting C-terminal sequence, -YKV-CO2−, reconstituted a functional PDZ class II–interacting motif present in Ephrin B receptors, a class that relies on interactions with the PDZ domain–containing proteins for clustering (Torres et al., 1998; Brückner et al., 1999; Lin et al., 1999; Madsen et al., 2005). Other constructs in the C-terminal XFP fusion series, mGAT1XFP3, mGAT1XFP8, mGAT1XFP20, mGAT1XFP28, and mGAT1XFP45, had the most C-terminal 3, 8, 20, 28, or 45 residues of the hGAT1 appended after the mGAT1XFP fusion. The differences in nucleotide sequence between the hGAT1 and mGAT1 C termini were a useful source of positive identification when the depositors analyzed the clones during construction. PCR integration was applied to amplify and insert EYFP or ECFP directly between residues R565 and L566, I570 and Q571, or V577 and R578 of mGAT1 to generate the mGAT15xxXFP5xxCT constructs. The site of XFP insertion in GAT1 is highlighted in the nomenclatures for these constructs by residue numbers flanking the fluorophore, and the “CT” denotes that the insertion occurs within the C terminus. Please see the associated article for more detailed information regarding construct creation and usage. | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | “Monomerizing” YFP A206K mutation; YFP inserted directly between residues V577 and R578 of mGAT1 | 2026-07-25 12:46:54 | 1 |
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pcDNA3.1(+)mGAT1-570-CFP-571CT Resource Report Resource Website 1+ mentions |
RRID:Addgene_41680 | Mus musculus GABA transporter 1 | Mus musculus | Ampicillin | PMID:19948998 | To generate the fluorescent mutants mGAT10XFP and mGAT1XFP* through mGAT1XFP45, the wild-type mGAT1 open reading frame (ORF) was subcloned without its original stop codon into the HindIII and EcoRI sites of the pcDNA3.1(+) expression vector multiple cloning site (MCS). XFP ORFs were then subcloned downstream from and in frame with the mGAT1 ORF at the NotI and XbaI sites of the pcDNA3.1(+) MCS. This resulted in a 12–amino acid spacer between the end of the mGAT1 sequence and the beginning of the fluorophore. The depositors modified a method for the integration of PCR fragments without the use of restriction enzymes (Geiser et al., 2001) to add the final 3, 8, 20, 28, or 45 codons of the human GAT1 (hGAT1) ORF. These were amplified from a source plasmid using the proof-reading PfuTurbo Cx Hotstart polymerase with 5′ and 3′ extensions corresponding to the 20–22-nt regions that flanked the intended site of insertion, such that the PCR product integrated in-frame immediately after the fluorophore sequence when used as the primers in a subsequent QuikChange II XL mutagenesis PCR reaction. For mGAT1XFP*, the depositors simply added a GTC codon for Val after the fluorophore ORF. The attached image displays the protein sequences of the modified regions of mGAT1 for each fluorescent construct. mGAT10CFP and mGAT10YFP repeated the fusion design of mGAT10GFP but with the fluorophore exchanged as annotated. The three C-terminal residues of the mGAT0XFP fusions are -YKI-CO2−, which comprises a broadly defined consensus PDZ class II–interacting motif (X-φ-X-φ, where φ designates a hydrophobic residue and X any residue) (Sheng and Sala, 2001; Hung and Sheng, 2002). The depositors searched the Ensembl databases using Biomart (http://www.ebi.ac.uk/biomart) (Spudich et al., 2007) and applied the GO:0005886 “plasma membrane” cellular component filter. The search identified no known membrane proteins possessing the -YKI-CO2− C-terminal sequence. In the mGAT1XFP* constructs, the depositors defined the terminal residue P(0) more narrowly, changing the terminal isoleucine residue present in mGAT10XFP to a valine in mGAT1XFP*. The resulting C-terminal sequence, -YKV-CO2−, reconstituted a functional PDZ class II–interacting motif present in Ephrin B receptors, a class that relies on interactions with the PDZ domain–containing proteins for clustering (Torres et al., 1998; Brückner et al., 1999; Lin et al., 1999; Madsen et al., 2005). Other constructs in the C-terminal XFP fusion series, mGAT1XFP3, mGAT1XFP8, mGAT1XFP20, mGAT1XFP28, and mGAT1XFP45, had the most C-terminal 3, 8, 20, 28, or 45 residues of the hGAT1 appended after the mGAT1XFP fusion. The differences in nucleotide sequence between the hGAT1 and mGAT1 C termini were a useful source of positive identification when the depositors analyzed the clones during construction. PCR integration was applied to amplify and insert EYFP or ECFP directly between residues R565 and L566, I570 and Q571, or V577 and R578 of mGAT1 to generate the mGAT15xxXFP5xxCT constructs. The site of XFP insertion in GAT1 is highlighted in the nomenclatures for these constructs by residue numbers flanking the fluorophore, and the “CT” denotes that the insertion occurs within the C terminus. Please see the associated article for more detailed information regarding construct creation and usage. | Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | “Monomerizing” CFP A206K mutation; CFP inserted directly between residues I570 and Q571 of mGAT1 | 2026-07-25 12:46:54 | 1 |
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pEXP5-NT-AlleyCat Resource Report Resource Website |
RRID:Addgene_41715 | mutated C-terminal portion of calmodulin gene (M76-K148) | Gallus gallus | Ampicillin | PMID:21482808 | Backbone Marker:Invitrogen; Backbone Size:2745; Vector Backbone:pEXP5-NT/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | F92E | 2026-07-25 12:46:55 | 0 |
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