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Species:
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:5997; Vector Backbone:BB3_AMA_2.8_pUC-ORI_L_AC_hph; Vector Types:Bacterial Expression, A. niger; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_90278 Copy
Species: A. niger
Genetic Insert: gRNA (pyrg2)
Vector Backbone Description: Backbone Size:5997; Vector Backbone:BB3_AMA_2.8_pUC-ORI_L_AC_hph; Vector Types:Bacterial Expression, A. niger; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_90277 Copy
Species: Discosoma sp
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5994; Vector Backbone:pMyCA; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_84272 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5994; Vector Backbone:pAL5000, pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_84268 Copy
Species:
Genetic Insert: sgRNA cloning site
Vector Backbone Description: Backbone Marker:Addgene 20318; Backbone Size:6608; Vector Backbone:pTE-10M-0X; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_84380 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Wilmanns lab; Backbone Size:6876; Vector Backbone:pMyC; Vector Types:Bacterial Expression, Bacterial expression vector; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_84688 Copy
Species:
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Sheng Yang lab; Backbone Size:3500; Vector Backbone:pAHYB; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_85074 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Sheng Yang Lab; Backbone Size:3500; Vector Backbone:pGHYB; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_87447 Copy
Species:
Genetic Insert: hyg
Vector Backbone Description: Backbone Marker:Yale Coli Genetic Stock Center; Backbone Size:6000; Vector Backbone:pCP20; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_87831 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_50367 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_50370 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_50371 Copy
Species: Homo sapiens
Genetic Insert: human Gamma 1 heavy chain expression cassette with unknown specificity
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_50383 Copy
Species:
Genetic Insert: GFP (including ATG start codon)
Vector Backbone Description: Backbone Marker:Koen De Smet Imperial College Medical School; Backbone Size:5304; Vector Backbone:pSODIT; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Plasmid can also be grown in mycobacteria.
This is a control plasmid for pSC301 in which the SOD promoter was excised.
Proper citation: RRID:Addgene_31852 Copy
Species:
Genetic Insert: int L5; xylEm; ttsbiA, ttsbiB
Vector Backbone Description: Vector Backbone:pML603; Vector Types:bacterial integration vector; Bacterial Resistance:Hygromycin
References:
Comments: This expression vector integrates at the mycobacteriophage L5 site and can be stabilized by excision of the vector backbone including the int gene using the Cre recombinase; contains xylE gene as a reporter for integration; terminators to protect expression cassette against transcriptional interference.
Proper citation: RRID:Addgene_32376 Copy
Species:
Genetic Insert: gfpm2+
Vector Backbone Description: Vector Backbone:pMS2; Vector Types:Mycobacterial GFP expression vector; Bacterial Resistance:Hygromycin
References:
Comments: This is an expression vector with a codon optimized gfp gene encoding an improved GFP (enhanced fluorescence, faster folding, more stable).
Additional reference for this construct: Steinhauer et al., 2010; Appl Environ Microbiol 76:546-554
Proper citation: RRID:Addgene_32362 Copy
Species: Homo sapiens
Genetic Insert: Human immunoglobulin gamma 1 constant region
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_52213 Copy
Species: Homo sapiens
Genetic Insert: Human immunoglobulin gamma 1 constant region
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_52214 Copy
Species:
Genetic Insert: n/a
Vector Backbone Description: Backbone Size:5708; Vector Backbone:pSMT3; Vector Types:Shuttle vector for mycobacteria; Bacterial Resistance:Hygromycin
References:
Comments: Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon. Tuberculosis (Edinb) 87: 481–488.
There are known discrepancies between the Addgene QC sequence and the depositor's full sequence--these are likely due to how the full sequence was assembled and should not affect function.
Proper citation: RRID:Addgene_26589 Copy
Species:
Genetic Insert: pmyc1tetO
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: pmyc1tetO is a tet operator containing promoter for mycobacteria. Also see Pubmed IDs 18059281 and 15687379.
Derivative of pMS2 containing Pmyc1tetO. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17972 Copy
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