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Species: Arabidopsis thaliana
Genetic Insert: 5mARF17
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGreenII0229; Vector Types:Plant Expression, plant transformation via Agrobacterium; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15829600
Comments: See "sequence" for full insert sequence. See article for cloning details.
Proper citation: RRID:Addgene_12071 Copy
Species: Arabidopsis thaliana
Genetic Insert: 5mARF17
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGreenII0129; Vector Types:Plant Expression, plant transformation via Agrobacterium; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15829600
Comments: See "sequence" for full insert sequence. See article for cloning details.
Proper citation: RRID:Addgene_12081 Copy
Species: Arabidopsis thaliana
Genetic Insert: SUC2 promoter
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2246bp
SUC2 promoter = 2252-4381bp
BsaI site #2 = 4387-4392bp
Proper citation: RRID:Addgene_122835 Copy
Species: Arabidopsis thaliana
Genetic Insert: SET catalytic domain of KYP H3K9 histone methyltransferase
Vector Backbone Description: Backbone Size:2688; Vector Backbone:pUC19; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2246bp
KYP coding sequence = 2254-3060bp
BsaI site #2 = 3066-3071bp
Proper citation: RRID:Addgene_122855 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G16900
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SLDCGLPSNEPPYIEPVTGLVFSSDADHIPSGISGRIQKNLEAVHIKPYLFLRYFPDGLRNCYTLDVLQNRRYMIKAVFVYGNYDGYNDYPSFDLYLGPNKWVRVDLEGKVNGSVEEIIHIPSSNSLQICLVKTGNSLPFISALELRLLRNDTYVVQDVSLKHLFRRYYRQSDRLIRYPDDVYDRVWSPFFLPEWTQITTSLDVNNSNNYEPPKAALTSAATPGDNGTRLTIIWTLDNPDEQIHLYVHFAELEPVGENTDEALRTLFTRTFYFVVNGKISYDESITPLDLAVSTVETVVNKCDGGNCSLQLVRSEASPGVRVPLVNAMEAFTAIKFPHSETNPDDVISIKVIQATYELSRVDWQGDPCLPQQFLWTGLNCSYMNMSTSPRIISLDLSSHKLTGKIVPDIQNLTQLQKLDLSNNKLTGGVPEFLANMKSLLFINLSNNNLVGSIPQALLDRKNLKLEFEGNPKLCATGPCNSSS
Proper citation: RRID:Addgene_114762 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT4G29180
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: QAGFISIDCGSPPNINYVDTDTGISYTWDAPFINAGVNLNVSEEYGYPKNPVLPFPLADVRSFPQGNRNCYTLTPSDGKGNLYLIRASFMYGNYDGKNALPEFDLYVNVNFWTSVKLRNASENVIKEILSFAESDTIYVCLVNKGKGTPFISALELRPMNSSIYGTEFGRNVSLVLYQRWDTGYLNGTGRYQKDTYDRIWSPYSPVSWNTTMTTGYIDIFQSGYRPPDEVIKTAASPKSDDEPLELSWTSSDPDTRFYAYLYFAELENLKRNESREIKIFWNGSPVSGAFNPSPEYSMTVSNSRAFTGKDHWISVQKTAESTRPPILNAIEIFSAQSLDEFYTRIDDVQAIESIKSTYKVNKIWTGDPCSPRLFPWEGIGCSYNTSSYQIKSLNLSSSGLHGPIAFAFRNLSLLESLDLSNNNLKGIVPEFLADLKYLKSLNLKGNNLTGFIPRSLRKRATANGLALSVDEQNICHSRSCR
Proper citation: RRID:Addgene_114760 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G59650
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SLDCGLPMTEPSSYTESVTGLRFSSDAEFIQTGESGKIQASMENDYLKPYTRLRYFPEERRNCYSLSVDKNRKYLIRARFIYGNYDGRNSNPIFELHLGPNLWATIDLQKFVNGTMEEILHTPTSNSLNVCLVKTGTTTPLISALELRPLGNNSYLTDGSLNLFVRIYLNKTDGFLRYPDDIYDRRWHNYFMVDDWTQIFTTLEVTNDNNYEPPKKALAAAATPSNASAPLTISWPPDNPGDQYYLYSHFSEIQDLQTNDTREFDILWDGAVVEEGFIPPKLGVTTIHNLSPVTCKGENCIYQLIKTSRSTLPSLLNALEIYTVIQFPRSETNENDVVAVKNIEAAYKLSRIRWQGDPCVPQKYAWDGLNCSNNTDVSKPPRVLSLNLSSSGLTGIIAAAIQNLTHLEKLDLSNNTLTGVVPEFLAQMKSLVIINLSGNNLSGPLPQGLRREGLELLVQGNPRLCLSGSCTEKNSKKK
Proper citation: RRID:Addgene_114764 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT2G28990
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SLDCGLPSDESPYDDSFNGLTFTSDSTFIQTGKIDSVDKDLNINLSKQYLTLRYFPEGKRNCYSLDVKRGTTYLIVVSFVYGNYDGLNRDPNFDIHLGPNKWKRIDLDGEKEGTREEIIHKARSNSLDICLVKTGETLPIISAIEIRPLRNNTYVTQSGSLMMSFRVYLSNSDASIRYADDVHDRIWSPFNGSSHTHITTDLNINNSNAYEIPKNILQTAAIPRNASAPLIITWDPLPINAEVYLYMHFAEIQTLEANETRQFDVILRGNFNHSGFSPTKLKVFTLYTEEPMKCGSEGCYLQLVKTPNSTLPPLINAIEAYSVIEFSQLETSLSDVDAIKNIKNTYKLNKITWQGDPCLPQDLSWESIRCTYVDGSTSPTIISLDLSKSGLNGSIPQILQNFTQLQELDLSNNSLTGPVPIFLANMKTLSLINLSGNNLSGSVPQALLDKEKEGLVLKLEGNPDLCKSSFCNTEKK
Proper citation: RRID:Addgene_114751 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT2G28960
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: QRGFISLDCGLPVNESPYTDPRTGLTFSSDADFILSGLRGEAGDDNTYIYRQYKDLRYFPDGIRNCYNLKVEQGINYLIRAGFGYGNYDGLNVYPKFDLHVGPNMWIAVDLEFGKDREIIYMTTSNLLQICLVKTGSTIPMISTLELRPLRNDSYLTQFGPLDLIYRRAYSSNSTGFIRYPDDIFDRKWDRYNEFETDVNTTLNVRSSSPFQVPEAVSRMGITPENASLPLRFYVSLDDDSDKVNVYFHFAEIQALRGNETREFDIELEEDIIQSAYSPTMLQSDTKYNLSPHKCSSGLCYLKLVRTPRSTLPPLISAIEAFKVVDFPYAETNPNDVAAMKDIEAFYGLKMISWQGDPCVPELLKWEDLKCSYTNKSTPPRIISLDLSSRGLKGVIAPAFQNLTELRKLDLSNNSFTGGVPEFLASMKSLSIINLNWNDLTGPLPKLLLDREKNGLKLTIQGNPKLCNDASCKNNNNQ
Proper citation: RRID:Addgene_114750 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G46330
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: QEGFISLDCGLPLNEPPYIESETGIQFSSDENFIQSGKTGRIPKNLESENLKQYATLRYFPDGIRNCYDLRVEEGRNYLIRATFFYGNFDGLNVSPEFDMHIGPNKWTTIDLQIVPDGTVKEIIHIPRSNSLQICLVKTGATIPMISALELRPLANDTYIAKSGSLKYYFRMYLSNATVLLRYPKDVYDRSWVPYIQPEWNQISTTSNVSNKNHYDPPQVALKMAATPTNLDAALTMVWRLENPDDQIYLYMHFSEIQVLKANDTREFDIILNGETINTRGVTPKYLEIMTWLTTNPRQCNGGICRMQLTKTQKSTLPPLLNAFEVYSVLQLPQSQTNEIEVVAIKNIRTTYGLSRISWQGDPCVPKQFLWDGLNCNITDISAPPRIISLNLSSSGLSGTIVSNFQNLAHLESLDLSNNSLSGIVPEFLATMKSLLVINLSGNKLSGAIPQALRDREREGLKLNVLGNKELCLSSTCIDKPKKK
Proper citation: RRID:Addgene_114754 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G21340
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SLDCGSLPNEPPYNDPSTGLTYSTDDGFVQSGKTGRIQKAFESIFSKPSLKLRYFPDGFRNCYTLNVTQDTNYLIKAVFVYGNYDGLNNPPSFDLYLGPNLWVTVDMNGRTNGTIQEIIHKTISKSLQVCLVKTGTSSPMINTLELRPLKNNTYNTQSGSLKYFFRYYFSGSGQNIRYPDDVNDRKWYPFFDAKEWTELTTNLNINSSNGYAPPEVVMASASTPISTFGTWNFSWLLPSSTTQFYVYMHFAEIQTLRSLDTREFKVTLNGKLAYERYSPKTLATETIFYSTPQQCEDGTCLLELTKTPKSTLPPLMNALEVFTVIDFPQMETNPDDVAAIKSIQSTYGLSKISWQGDPCVPKQFLWEGLNCNNLDNSTPPIVTSLNLSSSHLTGIIAQGIQNLTHLQELDLSNNNLTGGIPEFLADIKSLLVINLSGNNFNGSIPQILLQKKGLKLILEGNANLICPDGLCVNKAGNGGAKKMNVVIPIVASVAFV
Proper citation: RRID:Addgene_114753 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT4G20450
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: QGFISLDCGMPRNESSYTDESTGLNFSSDADFISSGKSGTIKTEDSDSGVKYIKPYKQLRYFPEGARNCYNLTVMQGTHYLIRAVFVYGNYDLKQRPKFDLYLGPNFWTTINLQDPSGGFYYRIWLQDGTVEEVIHMPKSNNLDICLVKTGTTTPFISSLELRPLRDDTYTTTTGSLKLISRWYFRKPFPTLESIIRHPDDVHDRLWDVYHADEEWTDINTTTPVNTTVNAFDLPQAIISKASIPQVASDTWSTTWSIQNPDDDVHVYLHFAEIQALKPSDTREFSILWNKNTIIRDYYSPLEFMADTVPIRTSSKCGDDGFCSLDLTRTKSSTLPPYCNAMEVFGLLQLLQTETDENDVTTLKNIQATYRIQKTNWQGDPCVPIQFIWTGLNCSNMFPSIPPRITSIDFSNFGLNGTITSDIQYLNQLQKLDLSNNNLTGKVPEFLAKMKLLTFINLSGNNLSGSIPQSLLNMEKNGLITLLYNGNNLCLDPSCESETGPGNNKKK
Proper citation: RRID:Addgene_114759 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G46370
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: MRNCYNLSVHKETKYLIRVTSNYGNYDGRNEPPRFDLYLGPNFWVTIDLGKHVNGDTWKEIIHIPKSNSLDVCLIKTGTTTPIISTLELRSLPKYSYNAISGSLKSTLRAFLSESTEVIRYPNDFYDRMWVPHFETEWKQISTNLKVNSSNGYLLPQDVLMTAAIPVNTSARLSFTENLEFPHDELYLYFHFSEVQVLQANQSREFSILWNGMVIYPDFIPDYLGAATVYNPSPSLCEVGKCLLELERTQKSTLPPLLNAIEVFTVMNFPQSETNDDDVIAITKIKDTHRLNRTSWQGDPCVPQLFSWAGLSCIDTNVSTPPRIISLNLSSSGLTGNIATGIQNLTKLQKLDLSNNNLTGVVPEFLANMKSLLFIDLRKNKLNGSIPKTLLDRKKKGLQLFVDGDDDKGDDNKCLSGSCVPKMK
Proper citation: RRID:Addgene_114757 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G51810
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: MWITVNTDNTIKEILHVSKSNTLQVCLVKTGTSIPYINTLELRPLADDIYTNESGSLNYLFRVYYSNLKGYIEYPDDVHDRIWKQILPYQDWQILTTNLQINVSNDYDLPQRVMKTAVTPIKASTTTMEFPWNLEPPTSQFYLFLHFAELQSLQANETREFNVVLNGNVTFKSYSPKFLEMQTVYSTAPKQCDGGKCLLQLVKTSRSTLPPLINAMEAYTVLDFPQIETNVDEVIAIKNIQSTYGLSKTTWQGDPCVPKKFLWDGLNCNNSDDSTPPIITSLNLSSSGLTGIIVLTIQNLANLQELDLSNNNLSGGVPEFLADMKSLLVINLSGNNLSGVVPQKLIEKKMLKLNIEGNPKLNCTVESCVNKDEEGGRQIKS
Proper citation: RRID:Addgene_114742 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT2G19210
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SGFVSIDCGIPEDSSYNDETTDIKYVSDAAFVESGTIHSIDPEFQTSSLEKQFQNVRSFPEGNRNCYDVKPPQGKGFKYLIRTRFMYGNYDNLGKAPDFDLYLGFNIWDSVTIDNATTIVTKEIIHTLRSDHVHVCLVDKNRGTPFLSALEIRLLKSNTYETPYDSLILFKRWDLGGLGALPVRYKDDVFDRIWIPLRFPKYTIFNASLTIDSNNNEGFQPARFVMNTATSPEDLSQDIIFSWEPKDPTWKYFVYMHFAEVVELPSNETREFKVLLNEKEINMSSFSPRYLYTDTLFVQNPVSGPKLEFRLQQTPRSTLPPIINAIETYRVNEFLQSPTDQQDVDAIMRIKSKYGVKKSWLGDPCAPVKYPWKDINCSYVDNESPRIISVNLSSSGLTGEIDAAFSNLTLLHILDLSNNSLTGKIPDFLGNLHNLTELNLEGNKLSGAIPVKLLERSNKKLILLRIDGNPDLCVSASCQISDEKTKK
Proper citation: RRID:Addgene_114748 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT2G19190
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SGFISIDCGIPDDSSYNDETTGIKYVSDSAFVDSGTTKRIAAQFQSSGFDRHLLNVRSFPQSKRSCYDVPTPRGKGFKYLIRTRFMYGNYDDLGRVPEFDLYLGVNFWDSVKLDDATTILNKEIITIPLLDNVQVCVVDKNAGTPFLSVLEIRLLLNTTYETPYDALTLLRRLDYSKTGKLPSRYKDDIYDRIWTPRIVSSEYKILNTSLTVDQFLNNGYQPASTVMSTAETARNESLYLTLSFRPPDPNAKFYVYMHFAEIEVLKSNQTREFSIWLNEDVISPSFKLRYLLTDTFVTPDPVSGITINFSLLQPPGEFVLPPIINALEVYQVNEFLQIPTHPQDVDAMRKIKATYRVKKNWQGDPCVPVDYSWEGIDCIQSDNTTNPRVVSLNISFSELRGQIDPAFSNLTSIRKLDLSGNTLTGEIPAFLANLPNLTELNVEGNKLTGIVPQRLHERSKNGSLSLRFGRNPDLCLSDSCSNTKKKNK
Proper citation: RRID:Addgene_114747 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT2G14510
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SQNQQGFISLDCGLPSKESYIEPSSNLTFISDVNFIRGGKTGNIQNNSRTNFIFKPFKVLRYFPDGIRNCYSLSVKQGTKYLIRTLFYYGNYDGLNTSPRFDLFLGPNIWTSVDVLIADVGDGVVEEIVHVTRSNILDICLVKTGTSTPMISAIELRPLRYDTYTARTGSLKSMAHFYFTNSDEAIRYPEDVYDRVWMPYSQPEWTQINTTRNVSGFSDGYNPPQGVIQTASIPTNGSEPLTFTWNLESSDDETYAYLFFAEIQQLKVNETREFKILANGVDYIDYTPWKFEARTLSNPAPLKCEGGVCRVQLSKTPKSTLPPLMNAIEIFSVIQFPQSDTNTDEVIAIKKIQSTYQLSRISWQGDPCVPKQFSWMGVSCNVIDISTPPRIISLDLSLSGLTGVISPSIQNLTMLRELDLSNNNLTGEVPEFLATIKPLLVIHLRGNNLRGSVPQALQDREKNDGLKLFVDPNITR
Proper citation: RRID:Addgene_114746 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT2G19230
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SGFVSIDCGIPEDSSYYDEKTDIKYISDAAFVESGTIHSIDSKFQKKNLEKQFQKVRSFPEGKKNCYDVQPPQGKGFKYLIRTRFMYGNYDNLGKAPDFDLYLGVNLWDSVTLENSTTIVTKEIIYTLRSDKVHVCLVDKERGTPFLSVLELRLLKNNIYETASDSLMLYRRWDLGATGDLPARYKDDIFDRFWMPLMFPNFLILNTSLMIDPTSSNGFLPPSVVMSTAVAPMNSSIEQIMVYWEPRDPNWKFYIYIHFAEVEKLPSNETREFSVFLNKEQIDTTSVFRPSYLYTDTLYVQNPVSGPFLEFVLRQGVKSTRPPIMNAIETYRTNEFLDLPTDQNDVDAIMKIKTKYKVKKNWLGDPCAPFGYPWQGINCSYTANNPPRIISVNLSFSGLTGQIDPVFITLTPLQKLDLSNNRLTGTVPDFLANLPDLTELNLEENKLTGILPEKLLERSKDGSLSLRVGGNPDLCVSDSCRNKKTER
Proper citation: RRID:Addgene_114749 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G07550
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SLDCGLASNESPYNEANSNLTYISDADFIQGGKTGNVQKDLLMKLRKPYTVLRYFPDGIRNCYSLNVKQDTNYLIRVMFRYGNYDGLNNSPRFDLYLGPNIWTTIDMGKSGDGVLEEIIHITRSNILDICLVKTGTSTPMISSIELRPLLYDTYIAQTGSLRNYNRFYFTDSNNYIRYPQDVHDRIWVPLILPEWTHINTSHHVIDSIDGYDPPQDVLRTGAMPANASDPMTITWNLKTATDQVYGYIYIAEIMEVQANETREFEVVVNNKVHFDPFRPTRFEAQVMFNNVPLTCEGGFCRLQLIKTPKSTLPPLMNAFEIFTGIEFPQSETNQNDVIAVKNIQASYGLNRISWQGDPCVPKQFLWTGLSCNVIDVSTPPRIVKLDLSSSGLNGVIPPSIQNLTQLQELDLSQNNLTGKVPEFLAKMKYLLVINLSGNKLSGLVPQALLDRKKEGLKLLVDENMICVSCGT
Proper citation: RRID:Addgene_114738 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G25560
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: GVNFEVVALIGIKSSLTDPHGVLMNWDDTAVDPCSWNMITCSDGFVIRLEAPSQNLSGTLSSSIGNLTNLQTVLLQNNYITGNIPHEIGKLMKLKTLDLSTNNFTGQIPFTLSYSKNLQYLRVNNNSLTGTIPSSLANMTQLTFLDLSYNNLSGPVPRSLAKTFNVMGNSQICPTGTEKDCNGTQPKPMSITLNSSQNKSSDGGTK
Proper citation: RRID:Addgene_114773 Copy
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