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Species: Homo sapiens
Genetic Insert: pri-let-7e
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51380 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51386 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51385 Copy
Species: Homo sapiens
Genetic Insert: Lin28A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51387 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7g
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51381 Copy
Species: Homo sapiens
Genetic Insert: DGCR8
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene Quality Control sequencing has found some discrepancies in the full plasmid sequence in the V5 tag and linker region of the plasmid. The V5 tag and DGCR8 insert are not effected.
Proper citation: RRID:Addgene_51383 Copy
Species: Synthetic
Genetic Insert: mNeptune2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5425; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51309 Copy
Species: Homo sapiens
Genetic Insert: NM_001039802.1
Vector Backbone Description: Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:Cdc42 3'UTR; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: miR-29 seed mutant (position: 578-584, 997-1003, TGCT->GTAG)
Proper citation: RRID:Addgene_51390 Copy
Species: Mus musculus
Genetic Insert: KIF3A
Vector Backbone Description: Backbone Size:4666; Vector Backbone:RFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_51397 Copy
Species: Drosophila melanogaster
Genetic Insert: Arp11
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_51399 Copy
Species: Synthetic
Genetic Insert: mNeptune2.5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5425; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_51310 Copy
Species: Homo sapiens
Genetic Insert: p85alpha 3'UTR
Vector Backbone Description: Backbone Marker:Promega (modified to have 3' multi cloning sites); Vector Backbone:pGL3-UC; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
References:
Comments: miR-29 seed mutant (TGCT->GTAG)
Proper citation: RRID:Addgene_51393 Copy
Species: Mus musculus
Genetic Insert: Trim25
Vector Backbone Description: Backbone Marker:Clontech (modified); Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: AK154543 (except for K238E (SNP)). For vector modification, we first inserted new multi cloning sites (MCS) between EcoRI and BamHI sites of pLVX-EF1a-AcGFP1-N1 (Clontech, 631983). Next, we removed coding sequences of AcGFP from the above vector by using XbaI sites, and inserted new sequences comprised of Kozak-ATG-3XMyc between BamHI and EcoRI sites.
Proper citation: RRID:Addgene_51394 Copy
Species: Homo sapiens
Genetic Insert: 4I79
Vector Backbone Description: Vector Backbone:pFBOH-LIC; Vector Types:baculovirus expression; Bacterial Resistance:Ampicillin
References:
Comments: http://www.thesgc.org/structures/4I79/
Proper citation: RRID:Addgene_51319 Copy
Species: Homo sapiens
Genetic Insert: 4H4H
Vector Backbone Description: Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/4H4H/
http://www.thesgc.org/structures/2RFI/
http://www.rcsb.org/pdb/explore/explore.do?structureId=3CZU
Proper citation: RRID:Addgene_51314 Copy
Species: Other
Genetic Insert: 4IFG
Vector Backbone Description: Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: http://www.thesgc.org/structures/4IFG/
Proper citation: RRID:Addgene_51322 Copy
Species: Other
Genetic Insert: 4IDI
Vector Backbone Description: Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/4IDI/
Proper citation: RRID:Addgene_51321 Copy
Species: Other
Genetic Insert: 2QG5
Vector Backbone Description: Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: http://www.thesgc.org/structures/2QG5/
Proper citation: RRID:Addgene_51331 Copy
Species: Synthetic
Genetic Insert: 2NQA
Vector Backbone Description: Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: http://www.thesgc.org/structures/2NQA/
Proper citation: RRID:Addgene_51330 Copy
Species: Other
Genetic Insert: 3DAX
Vector Backbone Description: Vector Backbone:pCW-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: http://www.thesgc.org/structures/3DAX/
Proper citation: RRID:Addgene_51333 Copy
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