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Species: E. coli
Genetic Insert: self-targeting gRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33450024
Comments: Please note this plasmid contains Illumina 2nd step primer capture sites within the insert sequence that can affect next generation sequencing results. We suggest using sanger sequencing when working with this plasmid.
Proper citation: RRID:Addgene_124221 Copy
Species: E. coli
Genetic Insert: SpCas9-HF1
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33450024
Proper citation: RRID:Addgene_124226 Copy
Species: Synthetic
Genetic Insert: vsfGFP-9
Vector Backbone Description: Backbone Marker:Stanley Falkow (PMID 9673287); Vector Backbone:pANT4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26742079
Comments: A 9aa linker in vGFP-9 enables reflexive binding of the Enhancer domain to the fused GFP domain.
Proper citation: RRID:Addgene_124294 Copy
Species: Homo sapiens
Genetic Insert: UBE2G1
Vector Backbone Description: Vector Backbone:pLCE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31300418
Proper citation: RRID:Addgene_124299 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeptune
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124332 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeptune
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124331 Copy
Species: Caenorhabditis elegans
Genetic Insert: gpa-2, Cerulean
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124337 Copy
Species: Homo sapiens
Genetic Insert: NFATc4 nuclear factor of activated T cells
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124290 Copy
Species: Homo sapiens
Genetic Insert: Collagen type VII alpha 1 chain
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124286 Copy
Species: Homo sapiens
Genetic Insert: NFATc3 nuclear factor of activated T cells
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124288 Copy
Species: Caenorhabditis elegans
Genetic Insert: tagBFP2
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124321 Copy
Species: Caenorhabditis elegans
Genetic Insert: tagBFP
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124320 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cerulean
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124323 Copy
Species: Homo sapiens
Genetic Insert: NFATc4 nuclear factor of activated T cells
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
Proper citation: RRID:Addgene_124289 Copy
Species: Caenorhabditis elegans
Genetic Insert: tagBFP2
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124322 Copy
Species: Caenorhabditis elegans
Genetic Insert: mOrange
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124325 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124327 Copy
Species: Caenorhabditis elegans
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124328 Copy
Species: Homo sapiens
Genetic Insert: LMNA (R377H)
Vector Backbone Description: Vector Backbone:mRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_124270 Copy
Species: Homo sapiens
Genetic Insert: LMNA (R482Q)
Vector Backbone Description: Vector Backbone:mRFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_124273 Copy
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