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Species: Synthetic
Genetic Insert: NBid-PhoCl1-CBid
Vector Backbone Description: Backbone Size:5350; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.10.419556v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_164050 Copy
Species: Homo sapiens
Genetic Insert: LMNA targeting sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164045 Copy
Species: Caenorhabditis elegans
Genetic Insert: sgTS5-sgTS6-sgTS7
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164040 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:7451; Vector Backbone:pQCXIZ; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164232 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:7451; Vector Backbone:pQCXIZ; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164233 Copy
Species: Homo sapiens
Genetic Insert: RPA70
Vector Backbone Description: Backbone Size:7879; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164231 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8367; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164237 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8312; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164235 Copy
Species: Methanosarcina flavescens
Genetic Insert: Mf BulKRS(human-opti)
Vector Backbone Description: Backbone Size:5988; Vector Backbone:pCMV; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: The insert gene-Mf BulKRS gene ID is 53688242. The genetic codon of the insert gene has been human optimized
Proper citation: RRID:Addgene_164196 Copy
Species: Synthetic
Genetic Insert: CamPARI2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4579; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164193 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8371; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164239 Copy
Species: Homo sapiens
Genetic Insert: GLI
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: GlI K12 contains the entire coding region of GLI (-79 to 3522) cloned into the EcoRI site of pBluescript KS. The GLI cDNA contains an internal EcoRI site so the insert should be excised by cleavage with HindIII (at 5' end of Gli cDNA) and XbaI (at 3' end of Gli cDNA).
Proper citation: RRID:Addgene_16419 Copy
Species: Homo sapiens
Genetic Insert: ACE2 extracellular domain
Vector Backbone Description: Backbone Marker:Addgene plasmids 12154; Vector Backbone:pCSCG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164222 Copy
Species: SARS-CoV
Genetic Insert: Spike S1 domain
Vector Backbone Description: Backbone Marker:Addgene plasmids 12154; Vector Backbone:pCSCG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164220 Copy
Species:
Genetic Insert: PGK-HygR & ZeoR
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: Part of vector system to facilitate and simplify the use of rAAV for creating KOs in human cells. The other vectors for this system, pAAV-MCS, pAAV-RC, and pHelper, can be purchased from Stratagene.
The sequence information is the sequence between linkers A and B.
See author's map and article for more information. In the author's map, restriction sites for the generation of pNeDaKO fragments used for fusion PCR are shown. A and B refer to linkers A and B; yellow arrows: loxP sites; PGK: phosphoglycerate kinase eukaryotic promoter; Hyg: hygromycin resistance gene; EM 7: EM7 prokaryotic promoter; Zeo: zeomycin resistance gene; Amp: ampicillin resistance gene.
Note that though the Hyg-R gene has a point mutation, the Vogelstein lab has used this construct to for Hyg selection extensively.
Proper citation: RRID:Addgene_16414 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Miguel Valvano and Silvia Cardona (Addgene plasmid # 113634); Backbone Size:7518; Vector Backbone:pSCrhaB2; Vector Types:Bacterial Expression; Bacterial Resistance:Trimethoprim
References:
Comments: This plasmid is derived from pSCrhaB2 (Addgene plasmid #113634).
Reference for pSCrhaB2:
Cardona ST, Valvano MA. Plasmid. 2005 Nov;54(3):219-28. doi: 10.1016/j.plasmid.2005.03.004
Proper citation: RRID:Addgene_164226 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8370; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164229 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Miguel Valvano and Silvia Cardona; Backbone Size:5461; Vector Backbone:pSC201; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Trimethoprim
References:
Comments: This plasmid is derived from pSC201, which itself was made by cloning oriR6K and oriT from pGPΩTp (available on Addgene as plasmid #130660). pGPΩTp constructed by Flannagan et al. (2007). Originally from University of Western Ontario.
Proper citation: RRID:Addgene_164227 Copy
Species: Homo sapiens
Genetic Insert: TREX1
Vector Backbone Description: Backbone Size:8370; Vector Backbone:pLenti CMV GFP Blast; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164228 Copy
Species:
Genetic Insert: sgcGAS
Vector Backbone Description: Backbone Size:9265; Vector Backbone:pU6 Cas9-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164253 Copy
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