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Genetic Insert: mCherry-FIREtag
Vector Backbone Description: Backbone Size:4005; Vector Backbone:pIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37640938
Proper citation: RRID:Addgene_205164 Copy
Species: Homo sapiens
Genetic Insert: PCGF2
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_007144.2 Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_144480 Copy
Genetic Insert: PINK1-mCherry-FIREtag
Vector Backbone Description: Backbone Size:4005; Vector Backbone:pIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37640938
Proper citation: RRID:Addgene_205174 Copy
Vector Backbone Description: Vector Backbone:pKD4; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Nonreplicating ORBIT integrating plasmid with WT attP (P1) sequence. Replicates in pir+ strains for cloning. FRT sites can be used to excise most of the backbone. Kanamycin resistant. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint. Depositing lab uses BW25141 strain for growth (https://cgsc.biology.yale.edu/Strain.php?ID=64690).
Proper citation: RRID:Addgene_205294 Copy
Genetic Insert: H2B-ECFP-FIREmate
Vector Backbone Description: Backbone Size:4005; Vector Backbone:pIRES; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37640938
Proper citation: RRID:Addgene_205171 Copy
Genetic Insert: Hygro-P2A-nLAP(BFP)
Vector Backbone Description: Vector Backbone:pBluescript KS-; Vector Types:Bacterial cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30797903
Proper citation: RRID:Addgene_194317 Copy
Species: Synthetic
Genetic Insert: ilvA-relE_RBS3_CB3
Vector Backbone Description: Vector Backbone:pJUMP24-T24; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37260076
Proper citation: RRID:Addgene_201534 Copy
Species: Synthetic
Genetic Insert: Empty Tp901 Target
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3512; Vector Backbone:pCR-BluntII-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37012288
Proper citation: RRID:Addgene_195885 Copy
Vector Backbone Description: Vector Backbone:pKD4; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Same as pInt_attP1_ampR, except this plasmid has the orthogonal attP5 sequence. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint. Depositing lab uses BW25141 strain for growth (https://cgsc.biology.yale.edu/Strain.php?ID=64690).
Proper citation: RRID:Addgene_205299 Copy
Vector Backbone Description: Vector Backbone:pKD4; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Same as pInt_attP1_chlorR, except this plasmid has the orthogonal attP3 sequence. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint. Depositing lab uses BW25141 strain for growth (https://cgsc.biology.yale.edu/Strain.php?ID=64690).
Proper citation: RRID:Addgene_205298 Copy
Vector Backbone Description: Vector Backbone:pKD4; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Nonreplicating ORBIT integrating plasmid with WT attP (P1) sequence. Replicates in pir+ strains for cloning. FRT sites can be used to excise most of the backbone. Ampicillin resistant. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint. Depositing lab uses BW25141 strain for growth (https://cgsc.biology.yale.edu/Strain.php?ID=64690).
Proper citation: RRID:Addgene_205296 Copy
Species: Homo sapiens
Genetic Insert: TEK receptor tyrosine kinase
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1-V5/His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28199182
Proper citation: RRID:Addgene_205611 Copy
Species: Arabidopsis thaliana
Genetic Insert: BR1
Vector Backbone Description: Backbone Size:5515; Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36324888
Proper citation: RRID:Addgene_192819 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: CreiLOV
Vector Backbone Description: Backbone Size:5515; Vector Backbone:pAAV-CAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36324888
Proper citation: RRID:Addgene_192818 Copy
Species: Homo sapiens
Genetic Insert: NR4A1
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_002135.4 Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_144416 Copy
Species: Homo sapiens
Genetic Insert: CALR ins5 (del1-197)-FLAG
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26817954
Proper citation: RRID:Addgene_204534 Copy
Species: Homo sapiens
Genetic Insert: CALR ins5 (del198-430)-FLAG
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26817954
Proper citation: RRID:Addgene_204538 Copy
Species: Homo sapiens
Genetic Insert: CALR ins5 (del18-197)-FLAG
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26817954
Proper citation: RRID:Addgene_204539 Copy
Species: Homo sapiens
Genetic Insert: CALR ins5 (del1-17)-FLAG
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26817954
Proper citation: RRID:Addgene_204533 Copy
Species: Homo sapiens
Genetic Insert: MPL-HA
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26817954
Proper citation: RRID:Addgene_204530 Copy
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