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Species: Synthetic
Genetic Insert: J1-BBa_J23117-sfGFP
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Backbone Size:4569; Vector Backbone:pUC18TminiTn7T-Gm; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Alternative names: pCDP009, pUC18TminiTn7T-Gm_J1-BBa_J23117-sfGFP_Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A/S101A)
Used for conjugation, with pTNS1 and pRK2013 helper plasmids, to integrate J1-BBa_J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) cassettes into gram-negative bacteria.
Point mutation in the FRT site was observed and remained applicable with counterselection by pCK255
Proper citation: RRID:Addgene_171139 Copy
Species: Photorhabdus luminescens
Genetic Insert: luxCDABE
Vector Backbone Description: Backbone Marker:Kenneth M. Peterson; Vector Backbone:pBBRMCS-5; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167131 Copy
Species: Synthetic
Genetic Insert: LuxR-mRFP
Vector Backbone Description: Vector Backbone:pBLxR5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167504 Copy
Species: Synthetic
Genetic Insert: TtgRAM-mRFP
Vector Backbone Description: Vector Backbone:pFTtR5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167508 Copy
Species: Synthetic
Genetic Insert: TetR-RFP
Vector Backbone Description: Vector Backbone:pFTR5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167507 Copy
Species: Synthetic
Genetic Insert: VanRAM-mRFP
Vector Backbone Description: Vector Backbone:pWVR5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167509 Copy
Species: Synthetic
Genetic Insert: VanRAM-mRFP
Vector Backbone Description: Vector Backbone:pIVR5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167510 Copy
Species: Synthetic
Genetic Insert: CinRAM-mKelly2
Vector Backbone Description: Vector Backbone:pFCiK5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167514 Copy
Species: Synthetic
Genetic Insert: LuxR-tdKatushka2
Vector Backbone Description: Vector Backbone:pFLxTd5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167519 Copy
Species: Synthetic
Genetic Insert: LuxR-Violacein
Vector Backbone Description: Vector Backbone:pBLxVi5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_167516 Copy
Species: Other
Genetic Insert: NSP1 124A/125A
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Mammalian Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_164523 Copy
Species: Lobophyllia hemprichii
Genetic Insert: mEos3.2
Vector Backbone Description: Vector Backbone:pMT85-2res; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_173894 Copy
Species: Lobophyllia hemprichii
Genetic Insert: mEos3.2
Vector Backbone Description: Vector Backbone:pMT85-2res; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_173895 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F.
Proper citation: RRID:Addgene_158628 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 3 position (Q93E, Q198K, and S409F). This plasmid has an additional Q148A and Q271A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q148A and a second mutagenesis to yield the double mutant.
Proper citation: RRID:Addgene_158633 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 2 positions (Q198K and S409F). This plamid has an additional Q271A mutation, and amino acid 93 (glutamate, E) was mutated back to the reference (glutamine, Q). Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) two times on the pEN_TT 3XFLAG-MAVS plasmid: one mutagenesis to yield pEN_TT 3XFLAG-MAVS-Q271A and a second mutagenesis to yield the double mutant. The clones displayed consistent off-target mutations at one of two points. Two clones with different off-target mutations were digested and the good pieces ligated together so to yield the final sequence.
Proper citation: RRID:Addgene_158632 Copy
Species: Homo sapiens
Genetic Insert: MAVS
Vector Backbone Description: Backbone Marker:Addgene #83274; Vector Backbone:pEN_TTmiR2c_3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments: The plasmid differs from the MAVS reference sequence at 3 positions: Q93E, Q198K, and S409F. This plasmid has an additional Q148A mutation. Gene point mutations were performed using the QuikChange Kit (Agilent Cat#200517) on the pEN_TT 3XFLAG-MAVS plasmid.
Proper citation: RRID:Addgene_158630 Copy
Species: Homo sapiens
Genetic Insert: LST2
Vector Backbone Description: Backbone Size:2926; Vector Backbone:pAceBAC2-HMF; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_220787 Copy
Species: Homo sapiens
Genetic Insert: PSMB5
Vector Backbone Description: Vector Backbone:pACEBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_217896 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:8000; Vector Backbone:BBR1; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_215342 Copy
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