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Species: Homo sapiens
Genetic Insert: Split GFP Po-Mt Short P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164116 Copy
Vector Backbone Description: Backbone Size:6736; Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9482916
Comments: Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
Proper citation: RRID:Addgene_16402 Copy
Species: Homo sapiens
Genetic Insert: Split YFP Po-ER Long P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164113 Copy
Vector Backbone Description: Backbone Size:30767; Vector Backbone:pAdEasy-2; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9482916
Comments: Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, pAdTrack-CMV) into the adenoviral plasmid pAdEasy-2.
Plasmids recombined in pAdEasy-2 should be packaged in 911E4 cells to make virus.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_16401 Copy
Species: Homo sapiens
Genetic Insert: Split YFP Po-ER Short P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164114 Copy
Species: Homo sapiens
Genetic Insert: Split GFP and YFP Mt-ER- Po Long P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164117 Copy
Genetic Insert: PGK-NeoR & ZeoR
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14704360
Comments: Part of vector system to facilitate and simplify the use of rAAV for creating KOs in human cells. The other vectors for this system, pAAV-MCS, pAAV-RC, and pHelper, can be purchased from Stratagene.
The sequence information is the sequence between linkers A and B.
See author's map and article for more information. In the author's map, restriction sites for the generation of pNeDaKO fragments used for fusion PCR are shown. A and B refer to linkers A and B; yellow arrows: loxP sites; PGK: phosphoglycerate kinase eukaryotic promoter; Neo: neomycin resistance gene; EM 7: EM7 prokaryotic promoter; Zeo: zeomycin resistance gene; Amp: ampicillin resistance gene.
Proper citation: RRID:Addgene_16413 Copy
Species: Homo sapiens
Genetic Insert: PTEN Neo targeting
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4000; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV, adeno-associated viral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14704360
Comments: This targeting vector yields 1-5% homologous recombinants in HCT116 and other cell lines. See author's map for more information.
Proper citation: RRID:Addgene_16411 Copy
Species: Mus musculus
Genetic Insert: MeCP2 Isoform 1
Vector Backbone Description: Vector Backbone:pAAV-EF1a; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31868585
Proper citation: RRID:Addgene_164062 Copy
Species: Homo sapiens
Genetic Insert: Split GFP Mt-ER Short P2A
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33247103
Proper citation: RRID:Addgene_164108 Copy
Species: Homo sapiens
Genetic Insert: CLGT#2-mCherry
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:9598; Vector Backbone:FUGW; Vector Types:Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33361384
Comments: From TCGA datasets, we annotated the genes specific to classical glioblastoma-subtype and identified corresponding cis-regulatory elements to generate synthetic reporters to trace subtype identity.
Proper citation: RRID:Addgene_164106 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33420033
Proper citation: RRID:Addgene_164013 Copy
Species: Synthetic
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:7700; Vector Backbone:lentiCas9-Blast plasmid (Addgene #52962); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164134 Copy
Species: Homo sapiens
Genetic Insert: NLRP1
Vector Backbone Description: Vector Backbone:pDB.His.MBP.3C ; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33420033
Proper citation: RRID:Addgene_164010 Copy
Species: Synthetic
Genetic Insert: NFkB promoter-Cre-T2A-mRuby3
Vector Backbone Description: Backbone Size:8000; Vector Backbone:lentiCas9-Blast plasmid (Addgene #52962); Vector Types:Mammalian Expression, Lentiviral, Cre/Lox, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164137 Copy
Species: Synthetic
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:7700; Vector Backbone:lentiCas9-Blast plasmid (Addgene #52962); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33539780
Proper citation: RRID:Addgene_164135 Copy
Species: Other
Genetic Insert: mRuby2
Vector Backbone Description: Backbone Marker:Connie Cepko (Addgene plasmid # 11151); Backbone Size:4877; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The original multiple cloning site was replaced by an extended MCS to improve usability of the plasmid for restriction cloning.
Proper citation: RRID:Addgene_164093 Copy
Species: Other
Genetic Insert: DsRed
Vector Backbone Description: Backbone Marker:Connie Cepko (Addgene plasmid # 11151); Backbone Size:4877; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The original multiple cloning site was replaced by an extended MCS to improve usability of the plasmid for restriction cloning.
Proper citation: RRID:Addgene_164091 Copy
Species: Homo sapiens
Genetic Insert: MGT#2-mCherry
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14884; Backbone Size:7956; Vector Backbone:FUGW; Vector Types:Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33361384
Comments: From TCGA datasets, we annotated the genes specific to mesenchymal glioblastoma-subtype and identified corresponding cis-regulatory elements to generate synthetic reporters to trace subtype identity.
Proper citation: RRID:Addgene_164097 Copy
Species: Homo sapiens
Genetic Insert: MGT#1-mVenus
Vector Backbone Description: Backbone Marker:David Baltimore (Addgene plasmid # 14883); Backbone Size:7956; Vector Backbone:FUGW; Vector Types:Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33361384
Comments: From TCGA datasets, we annotated the genes specific to mesenchymal glioblastoma-subtype and identified corresponding cis-regulatory elements to generate synthetic reporters to trace subtype identity.
Proper citation: RRID:Addgene_164094 Copy
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