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Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRS423; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163973 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRS423; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163972 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6850; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
References:
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD122.75, Ligation number L4066.
Proper citation: RRID:Addgene_1640 Copy
Species: Homo sapiens
Genetic Insert: ASC
Vector Backbone Description: Backbone Marker:Addgene #41392; Vector Backbone:pLEX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163977 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:Addgene #41392; Vector Backbone:pLEX307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163976 Copy
Species: Homo sapiens
Genetic Insert: PKG1 alpha
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_16391 Copy
Species: Drosophila melanogaster
Genetic Insert: Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
Vector Backbone Description: Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: To generate a basolateral GrabFP construct that exposes the nanobody to the cytosol we started with the GrabFP-BExt plasmid. The tagBFP sequence was replaced by the vhhGFP4 sequence and the original vhhGFP4 sequence was exchanged with an mCherry coding sequence.
pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).
vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.
mCherry: Clonetech
Proper citation: RRID:Addgene_163926 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163992 Copy
Species: Homo sapiens
Genetic Insert: PKG1 beta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The PKG I beta insert aligns with the listed reference sequence after amino acid 90, contains an EF insertion after the starting methionine and I537V mutation.
Proper citation: RRID:Addgene_16394 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163997 Copy
Species: Homo sapiens
Genetic Insert: NLRP1 CARD
Vector Backbone Description: Vector Backbone:pcDNA3.1 LIC 6B; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_163996 Copy
Species: Enterobacteria phage T4
Genetic Insert: gp32
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_163912 Copy
Species: Synthetic
Genetic Insert: tRNA synthetase
Vector Backbone Description: Backbone Size:2507; Vector Backbone:pRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_163915 Copy
Species: Enterobacteria phage T4
Genetic Insert: uvsY
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_163914 Copy
Species: E. coli
Genetic Insert: beta-galactosidase with stuffer
Vector Backbone Description: Backbone Size:30000; Vector Backbone:pAdEasy-2; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments: beta-gal with stuffer (total 10kb) was cloned into pAdTrack-CMV, then recombined into pAdEasy-2.
AdEasy is a registered trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_16407 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:9220; Vector Backbone:pAdTrack-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments: Vector pAdTrack-CMV is used for expression of transgenes under a CMV promoter when a GFP tracer is desired.
Max insert size = 5.0kb with pAdEasy1 and 7.7kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
Proper citation: RRID:Addgene_16405 Copy
Species: Homo sapiens
Genetic Insert: Split GFP and YFP PM- Mt-ER Short P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164110 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8320; Vector Backbone:pAdTrack; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments: Vector pAdTrack is used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.
Max insert size = 5.9kb with pAdEasy1 and 8.6kb with pAdEasy2.
See attached protocol and http://www.coloncancer.org/adeasy.htm
for more information.
Proper citation: RRID:Addgene_16404 Copy
Species: Homo sapiens
Genetic Insert: Split GFP Po-Mt Long P2A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_164115 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:7452; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
References:
Comments: Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
Max insert size = 6.6kb with pAdEasy1 and 9.3kb with pAdEasy2.
See AdEasy Protocol linked in the supplemental documents section above for more information.
NOTE: There are discrepancies between the Addgene quality control sequence and the assembled sequence from the Vogelstein lab. These differences are not known to affect function.
Proper citation: RRID:Addgene_16403 Copy
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