Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Species: Mus musculus
Genetic Insert: alpha myosin heavy chain promoter
Vector Backbone Description: Backbone Size:8400; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19352491
Proper citation: RRID:Addgene_21229 Copy
Species: Mus musculus
Genetic Insert: alpha myosin heavy chain promoter
Vector Backbone Description: Backbone Size:7338; Vector Backbone:SMPU; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19352491
Comments: There is an extra C in the promoter sequence when compared to the available reference sequence in NCBI. The mismatch is due to older sequencing artifact of an erroneous insertion of a "C" adjacent to "GGG". The construct should be correct.
Proper citation: RRID:Addgene_21228 Copy
Genetic Insert: GateWay conversion cassette
Vector Backbone Description: Backbone Size:14820; Vector Backbone:pRosa26-PA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19014667
Comments: pRosa26-DEST was made by inserting a GateWay conversion cassette into the XhoI site of pBigT, followed by transfer of PacI / AscI fragment of the resulting vector to the pRosa26-PA vector (see http://www.biomedcentral.com/1471-213X/1/4
for details on these vectors). The resulting vector resembles the construct that was used for the R26R Cre reporter mouse made by Phil Soriano in which the expression of lacZ is coupled to the endogenous Rosa26 locus via a splice acceptor site, but only after removal of the lox-STOP-lox cassette (which is actually the neo-R cassette used during the targeting). The only thing changed from the pBigT vectors and the R26R reporter is the cloning strategy, so the resulting knock-in should be as good as these systems, and as good (or as bad) as the use of the Rosa26 locus in any situation.
Stbl3 cells are advisable to use after users have done the LR recombination reaction.
See http://www.hgu.mrc.ac.uk/people/n.hastie_rosa.html
for more information and protocols for this plasmid.
Further advice from the depositing lab:
"Grow single colonies in Amp + Cam at 30 C, definitively not 37. Including the Cam should ensure that spontaneous recombinants that loose the Gateway cassette don't survive...
- Do minipreps and test with the double digest PacI / AscI. This should give 4.9 kb + 9.8 kb. If this is okay, the vector should be okay"
Please note that there are discrepancies between Addgene's quality control sequence and the theoretical sequence supplied by the depositing lab. These discrepancies should not affect the function of the construct.
Proper citation: RRID:Addgene_21189 Copy
Species: Rattus norvegicus
Genetic Insert: CAMKIIalpha-T286A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10102820
Proper citation: RRID:Addgene_21220 Copy
Species: Rattus norvegicus
Genetic Insert: CAMKIIalpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4725; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9768845
Proper citation: RRID:Addgene_21226 Copy
Species: Rattus norvegicus
Genetic Insert: CaMKIIbeta(A303R)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10102820
Proper citation: RRID:Addgene_21224 Copy
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5371; Vector Backbone:pAc5.1C; Vector Types:Drosophila expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16177138
Proper citation: RRID:Addgene_21182 Copy
Genetic Insert: Enhanced Green Fluorescence Protein
Vector Backbone Description: Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6531; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19404254
Comments: EGFP driven by EOS-S(4+) promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).
Proper citation: RRID:Addgene_21317 Copy
Genetic Insert: monomeric Red Fluorescence Protein 1
Vector Backbone Description: Backbone Size:4582; Vector Backbone:pMXs MoMLV-based retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19404254
Comments: mRFP1 (maximum Excitation wave length, 584 nm; maximum Emission wave length, 607 nm). pMXs MoMLV-based retroviral vector from Dr. Toshio Kitamura (Institute of Medical Science, University of Tokyo).
Proper citation: RRID:Addgene_21315 Copy
Species: Mus musculus
Genetic Insert: Axin2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:For riboprobe synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Comments: Tong DNA #94. The DNA is a HinDIII fragment of mouse Axin2 (nucleotides 1-2397) in pBluescript-KS. The antisense and sense Axin2 riboprobes were made by linearizing this plasmid with NotI or SalI and transcribing with T3 or T7 RNA polymerase, respectively. List of DNAs says " ~2 kb DIII fragment of Axin2 (1-2397) in pBS-KS”.
Proper citation: RRID:Addgene_21277 Copy
Species: Mus musculus
Genetic Insert: Axin2 promoter
Vector Backbone Description: Backbone Marker:Clontech (discontinued); Backbone Size:4300; Vector Backbone:d2EGFP-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11809808
Comments: The 5.6-kb DNA fragment was excised from Axin2-Luciferase using KpnI and HindIII (the HindIII site was filled in with Klenow DNA polymerase) and cloned into the KpnI- and SmaI-digested pd2EGFP-1 vector.
Proper citation: RRID:Addgene_21276 Copy
Species: Mus musculus
Genetic Insert: Axin2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Comments: A 5.6-kb DNA fragment containing the Axin2 promoter, exon 1, intron 1, and part of exon 2 was subcloned from a mouse BAC clone and sequenced. In Author's map, exons are shown as gray boxes, and the sequence is numbered with bp 1 corresponding to the start of the cDNA sequence. Eight conserved Tcf/LEF
binding sites (underlined and designated T1 to T8) were identified.
Proper citation: RRID:Addgene_21275 Copy
Genetic Insert: Enhanced Green Fluorescence Protein, Puromycin resistance gene
Vector Backbone Description: Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6626; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19404254
Comments: EGFP and Puro resistance genes driven by EOS-S(4+) promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).
Proper citation: RRID:Addgene_21314 Copy
Species: Mus musculus
Genetic Insert: Axin2
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Proper citation: RRID:Addgene_21279 Copy
Species: Mus musculus
Genetic Insert: Axin2
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Comments: Full-length Axin2 cDNA. Tong clone #99.
Proper citation: RRID:Addgene_21278 Copy
Species: Homo sapiens
Genetic Insert: HIF1AN
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12615973
Proper citation: RRID:Addgene_21399 Copy
Species: Caenorhabditis elegans
Genetic Insert: cye-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21392 Copy
Species: Mus musculus
Genetic Insert: Int3
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pLNC; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9576833
Proper citation: RRID:Addgene_21329 Copy
Genetic Insert: Enhanced Green Fluorescence Protein
Vector Backbone Description: Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6531; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19404254
Comments: EGFP driven by Nanog promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).
Proper citation: RRID:Addgene_21321 Copy
Species: Mus musculus
Genetic Insert: Axin
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9230313
Proper citation: RRID:Addgene_21287 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the PRECISE-TBI Resources search. From here you can search through a compilation of resources used by PRECISE-TBI and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that PRECISE-TBI has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on PRECISE-TBI then you can log in from here to get additional features in PRECISE-TBI such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into PRECISE-TBI you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within PRECISE-TBI that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
