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Species: Synthetic
Genetic Insert: ie1-EGFP
Vector Backbone Description: Vector Backbone:Janelia Atalanta cloning plasmid; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.17.545412v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_204301 Copy
Species: Synthetic
Genetic Insert: 3XP3-ECFP
Vector Backbone Description: Vector Backbone:Janelia Atalanta cloning plasmid; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_204302 Copy
Species: Synthetic
Genetic Insert: blasticidine - P2A - TETon-3G transactivator
Vector Backbone Description: Backbone Marker:Vlatka Zoldos; Backbone Size:8020; Vector Backbone:#71666 pdCas9-DNMT3A-EGFP; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36307419
Comments: A lentiviral UbC-blasticidine-P2A-TETon-3G transactivator vector was synthesized using the #71666 pdCas9-DNMT3A-EGFP vector backbone digested with NheI-EcoRI and ligated to the following DNA fragments: UbC promoter and blasticidine cassette amplified and fused by PCR using 40–50 bp internal overlapping primers and one external reverse primer containing a P2A linker, and a TETon-3G fragment amplified from the Addgene vector #96963 pCAG-TetON-3G using a forward primer containing a P2A sequence so it could be fused to the UbC-Blasticidin-P2A sequence during a last round PCR amplification with NheI and EcoRI external primers.
Proper citation: RRID:Addgene_200191 Copy
Vector Backbone Description: Backbone Marker:Addgene ID 60648; Vector Backbone:pcDNA3.1(+) CircRNA Mini Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: EcoRI (not destroyed), 3' Cloning Site: XhoI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. For cloning cDNA, the 5' restriction site should be either NheI, BmtI, AflII, HindIII, Acc65I, KpnI, BamHI, BstXI or EcoRI and the 3' restriction site should be either XhoI, PspXI, XbaI, EcoO109I, PspOMI or ApaI. The neoR gene of Addgene plasmid ID 60648 also was replaced with the blastR gene from Addgene plasmid ID 98293. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206217 Copy
Vector Backbone Description: Backbone Marker:Addgene ID 60648; Vector Backbone:pcDNA3.1(+) CircRNA Mini Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: EcoRI (not destroyed), 3' Cloning Site: XhoI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. For cloning cDNA, the 5' restriction site should be either NheI, BmtI, AflII, HindIII, Acc65I, KpnI, BamHI or BstXI and the 3' restriction site should be either XhoI, PspXI, XbaI, EcoO109I, PspOMI or ApaI. The neoR gene of Addgene plasmid ID 60648 also was replaced with the hygroR gene from Addgene plasmid ID 98291. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206216 Copy
Species: Drosophila melanogaster
Genetic Insert: T2A-split-intein-Gal4[1-20]-N-int-BlastR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:PCR Blunt II - TOPO; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37276389
Proper citation: RRID:Addgene_199197 Copy
Genetic Insert: T2A-split-intein-Gal4[21-881]-C-int-G418R
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:PCR Blunt II - TOPO; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37276389
Proper citation: RRID:Addgene_199198 Copy
Species: Drosophila melanogaster
Genetic Insert: T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:PCR Blunt II - TOPO; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37276389
Proper citation: RRID:Addgene_199195 Copy
Species: Drosophila melanogaster
Genetic Insert: T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:PCR Blunt II - TOPO; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37276389
Proper citation: RRID:Addgene_199196 Copy
Species: Drosophila melanogaster
Genetic Insert: T2A-ZpGal4DBD
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:PCR Blunt II - TOPO; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37276389
Proper citation: RRID:Addgene_199199 Copy
Vector Backbone Description: Backbone Marker:Addgene ID 60648; Vector Backbone:pcDNA3.1(+) CircRNA Mini Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: EcoRI (not destroyed), 3' Cloning Site: XhoI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. For cloning cDNA, the 5' restriction site should be either NheI, BmtI, AflII, HindIII, Acc65I, KpnI, Eco53kI, SacI, BamHI, BstXI or EcoRI and the 3' restriction site should be either XhoI, PspXI or XbaI. The neoR gene of Addgene plasmid ID 60648 also was replaced with the blastR gene from Addgene plasmid ID 98293. The CMV enhancer/promoter of Addgene plasmid ID 60648 also was replaced with the hPGK promoter from Addgene plasmid ID 96923. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206229 Copy
Vector Backbone Description: Backbone Marker:Addgene ID 60648; Vector Backbone:pcDNA3.1(+) CircRNA Mini Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: EcoRI (not destroyed), 3' Cloning Site: XhoI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. For cloning cDNA, the 5' restriction site should be either NheI, BmtI, AflII, HindIII, Acc65I, KpnI, Eco53kI, SacI, BamHI, SpeI, BstXI or EcoRI and the 3' restriction site should be either XhoI, PspXI, XbaI, PspOMI or ApaI. The neoR gene of Addgene plasmid ID 60648 also was replaced with the blastR gene from Addgene plasmid ID 98293. The CMV enhancer/promoter of Addgene plasmid ID 60648 also was replaced with the EF1a promoter from Addgene plasmid ID 98290. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206223 Copy
Vector Backbone Description: Backbone Marker:Addgene ID 60648; Vector Backbone:pcDNA3.1(+) CircRNA Mini Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: EcoRI (not destroyed), 3' Cloning Site: XhoI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. For cloning cDNA, the 5' restriction site should be either NheI, BmtI, AflII, HindIII, Acc65I, KpnI, Eco53kI, SacI, BamHI, SpeI, BstXI or EcoRI and the 3' restriction site should be either XhoI, PspXI, XbaI, EcoO109I, PspOMI or ApaI. The CMV enhancer/promoter of Addgene plasmid ID 60648 also was replaced with the EF1a promoter from Addgene plasmid ID 98290. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206220 Copy
Vector Backbone Description: Backbone Marker:Addgene ID 60648; Vector Backbone:pcDNA3.1(+) CircRNA Mini Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: EcoRI (not destroyed), 3' Cloning Site: XhoI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. For cloning cDNA, the 5' restriction site should be either NheI, BmtI, AflII, HindIII, Acc65I, KpnI, Eco53kI, SacI, BamHI, BstXI or EcoRI and the 3' restriction site should be either XhoI, PspXI or XbaI. The neoR gene of Addgene plasmid ID 60648 also was replaced with the puroR gene from Addgene plasmid ID 98290. The CMV enhancer/promoter of Addgene plasmid ID 60648 also was replaced with the hPGK promoter from Addgene plasmid ID 96923. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206227 Copy
Vector Backbone Description: Backbone Marker:Addgene ID 60648; Vector Backbone:pcDNA3.1(+) CircRNA Mini Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: EcoRI (not destroyed), 3' Cloning Site: XhoI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. For cloning cDNA, the 5' restriction site should be either NheI, BmtI, AflII, HindIII, Acc65I, KpnI, Eco53kI, SacI, BamHI, BstXI or EcoRI and the 3' restriction site should be either XhoI, PspXI or XbaI. The CMV enhancer/promoter of Addgene plasmid ID 60648 also was replaced with the hPGK promoter from Addgene plasmid ID 96923. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206226 Copy
Vector Backbone Description: Backbone Marker:Addgene ID 60648; Vector Backbone:pcDNA3.1(+) CircRNA Mini Vector; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38045421
Comments: ZKSCAN1 intron 1 (partial) & ZKSCAN1 intron 3 (partial) separated by a pair of BsmBI restriction sites. 5' Cloning Site: EcoRI (not destroyed), 3' Cloning Site: XhoI (not destroyed). The mc2 and pF mc2 series of plasmids were designed and created by Dr Brett Stringer in the laboratory of Prof Simon Conn. They are based on the original circRNA mini vector design of Liang & Wilusz, 2014. The pair of BsmBI restriction sites is the cloning site for DNA specifying the RNA sequence to be circularised. For cloning cDNA, the 5' restriction site should be either NheI, BmtI, AflII, HindIII, Acc65I, KpnI, Eco53kI, SacI, BamHI, SpeI, BstXI or EcoRI and the 3' restriction site should be either XhoI, PspXI, XbaI, EcoO109I, PspOMI or ApaI. The neoR gene of Addgene plasmid ID 60648 also was replaced with the mCherry gene from Addgene plasmid ID 155258. The CMV enhancer/promoter of Addgene plasmid ID 60648 also was replaced with the EF1a promoter from Addgene plasmid ID 98290. Please visit https://doi.org/10.1101/2023.11.21.568171 for bioRxiv preprint.
Proper citation: RRID:Addgene_206225 Copy
Species: Synthetic
Genetic Insert: MHC-EGFP+H3:H26
Vector Backbone Description: Vector Backbone:Janelia Atalanta cloning plasmid; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.06.17.545412v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_204289 Copy
Genetic Insert: Enhanced Green Fluorescent Protein (eGFP)
Vector Backbone Description: Vector Backbone:3rd gen lentiviral vector; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36864202
Proper citation: RRID:Addgene_194352 Copy
Species: Homo sapiens
Genetic Insert: two sgRNAs (each with a U6 promoter) to delete intronic SVA within SCN8A gene
Vector Backbone Description: Backbone Size:12790; Vector Backbone:modified version of 98291; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36997626
Proper citation: RRID:Addgene_202826 Copy
Genetic Insert: TVA-mCherry fusion protein
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36864202
Proper citation: RRID:Addgene_194354 Copy
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