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Species: E.coli
Genetic Insert: KanamycinResistance-RpsL
Vector Backbone Description: Backbone Size:2935; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:19160076
Proper citation: RRID:Addgene_20871 Copy
Species: Homo sapiens
Genetic Insert: HOXB6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6500; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15269212
Comments: Product of N->A mutation is a DNA-binding mutant.
Has IRES-GF
Proper citation: RRID:Addgene_20992 Copy
Species: Mus musculus
Genetic Insert: Inhibitor of DNA binding 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19896442
Proper citation: RRID:Addgene_20965 Copy
Species: Mus musculus
Genetic Insert: Inhibitor of DNA binding 1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19896442
Proper citation: RRID:Addgene_20964 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19896442
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Orange cDNA was obtained from Dr. Roger Tsien.
Proper citation: RRID:Addgene_20969 Copy
Genetic Insert: Firefly luciferase shRNA
Vector Backbone Description: Backbone Size:7471; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_20962 Copy
Genetic Insert: Gateway Destination Vector
Vector Backbone Description: Backbone Size:6465; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19252478
Proper citation: RRID:Addgene_20960 Copy
Species: Mus musculus
Genetic Insert: HOXA9
Vector Backbone Description: Backbone Size:6500; Vector Backbone:MSCV-PGK-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18474618
Comments: Addgene has detected several mutations in the UTR region of this construct.
Proper citation: RRID:Addgene_20977 Copy
Species: Mus musculus
Genetic Insert: HOXA9
Vector Backbone Description: Vector Backbone:MSCV (murine stem cell virus); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18474618
Comments: *Please note that the full sequence verified by Addgene differs from the original depositor's map and thus the numbers and nomenclature used by the depositor may differ from the Addgene map.*
Depositor's Comments:
We discovered that, although a 1,975-nt full-length Hoxa9 clone (numbered from the protein start site; I.M.A.G.E. clone BC055059) would produce protein when transiently expressed from the murine stem cell virus (MSCV) long terminal repeat, following retroviral transduction, the HOXA9 protein could not be detected. Since this cDNA contained two putative polyadenylation signals in the 3' UTR, we removed these sites to determine if they were interfering with retrovirally mediated protein expression. The site at nt 1798 was replaced using a primer containing a KpnI sequence in place of the AATAAA sequence together with a primer containing the BglII site at nt 749 to amplify a 1,052-nt BglII-KpnI fragment by PCR. A second primer containing the KpnI site in place of the polyadenylation signal at nt 1798 was used in conjunction with a primer containing an AAT-to-CGG change in the polyadenylation signal at nt 1975 and an adjacent XhoI site for cloning to generate a 150-nt KpnI-XhoI fragment. Standard methods were used to combine these fragments to create a 1,975-nt Hoxa9 cDNA encoding an N-terminal FLAG-tagged version of the mature protein but lacking the two putative polyadenylation signals. The resulting MSCV clone produced high levels of FLAG-HOXA9 protein and immortalized BM cells.
Proper citation: RRID:Addgene_20975 Copy
Species: Rattus norvegicus
Genetic Insert: Epsin1 deltaUIM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18689690
Proper citation: RRID:Addgene_20973 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19896442
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Venus cDNA was obtained from Dr. Anna-Katerina Hadjantonakis.
Proper citation: RRID:Addgene_20971 Copy
Species: Homo sapiens
Genetic Insert: Histone cluster 1, H2bb
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19896442
Comments: The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Strawberry cDNA was obtained from Dr. Roger Tsien.
Proper citation: RRID:Addgene_20970 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Size:9250; Vector Backbone:pLenti-CaMKIIa; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17410168
Comments: Please see http://www.optogenetics.org
for additional information.
H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
Proper citation: RRID:Addgene_20944 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pLenti-EF1a; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17410168
Comments: Please see http://www.optogenetics.org
for additional information.
The H134R mutation present in ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
Proper citation: RRID:Addgene_20942 Copy
Species: synthetic
Genetic Insert: opto-b2AR
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19295515
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20948 Copy
Species: synthetic
Genetic Insert: opto-a1AR
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19295515
Comments: Please see http://www.optogenetics.org
for additional information.
Proper citation: RRID:Addgene_20947 Copy
Species: C. reinhardtii
Genetic Insert: channelrhodopsin-2
Vector Backbone Description: Backbone Size:5545; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17410168
Comments: The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
Proper citation: RRID:Addgene_20940 Copy
Species: V. carteri
Genetic Insert: volvox channelrhodopsin-1
Vector Backbone Description: Backbone Size:5545; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18432196
Proper citation: RRID:Addgene_20955 Copy
Vector Backbone Description: Backbone Size:7424; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_20953 Copy
Species: Mus musculus
Genetic Insert: cMyc, KLF4, Oct4, Sox2
Vector Backbone Description: Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19252478
Comments: Additional reference, Kaji et al Nature. 2009 Mar 1
Proper citation: RRID:Addgene_20959 Copy
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