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Vector Backbone Description: Backbone Size:11779; Vector Backbone:pSEVA227C; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_193712 Copy
Genetic Insert: P*-loxP-TT-loxP-R-knt
Vector Backbone Description: Backbone Size:2067; Vector Backbone:pBbA-c; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36823420
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_189850 Copy
Genetic Insert: P-loxP-TT-loxP-R*-knt
Vector Backbone Description: Backbone Size:2067; Vector Backbone:pBbA-c; Vector Types:Bacterial Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36823420
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_189852 Copy
Species: Other
Genetic Insert: I-WcaI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Expresses the I-WcaI homing endonuclease from Wickerhamomyces canadensis. The sequence is codon optimized for expression in E. coli. The expressed protein contains a K125I mutation that results in DNA nicking activity. It contains an amino-terminal histidine tag for purification.
Proper citation: RRID:Addgene_195619 Copy
Species: Brachypodium (Bradi4g13987.1.p)
Genetic Insert: ID#50
Vector Backbone Description: Vector Backbone:pTWIST ENTRY; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37248633
Proper citation: RRID:Addgene_193550 Copy
Species: Synthetic
Genetic Insert: 92-1 Op (Part 2)
Vector Backbone Description: Backbone Size:1658; Vector Backbone:pYKT001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36367334
Proper citation: RRID:Addgene_194487 Copy
Species: Mus musculus
Genetic Insert: Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
Vector Backbone Description: Backbone Marker:Synthetic; Vector Backbone:Derived from pSLCAR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37347762
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.08.17.504342v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190680 Copy
Species: Mus musculus
Genetic Insert: Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag
Vector Backbone Description: Backbone Marker:Synthetic; Vector Backbone:Derived from pSLCAR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37347762
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.08.17.504342v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190681 Copy
Species: Homo sapiens
Genetic Insert: miRFP718nano-clathrin light chain
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36456785
Proper citation: RRID:Addgene_195747 Copy
Species: Homo sapiens
Genetic Insert: miRFP718nano-human alpha-tubulin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36456785
Proper citation: RRID:Addgene_195746 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1-miRFP718nano
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36456785
Proper citation: RRID:Addgene_195749 Copy
Species: Homo sapiens
Genetic Insert: miRFP718nano-human beta-actin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36456785
Proper citation: RRID:Addgene_195745 Copy
Vector Backbone Description: Backbone Size:10899; Vector Backbone:pSEVA227C; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_193726 Copy
Species: E. coli
Genetic Insert: galK
Vector Backbone Description: Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_193727 Copy
Species: Other
Genetic Insert: I-WcaI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Expresses the I-WcaI homing endonuclease from Wickerhamomyces canadensis. The sequence is codon optimized for expression in E. coli. The expressed protein contains a K226I mutation that results in DNA nicking activity. It contains an amino-terminal histidine tag for purification.
Proper citation: RRID:Addgene_195620 Copy
Species: Brachypodium (Bradi5g22162.1.p)
Genetic Insert: ID#58
Vector Backbone Description: Vector Backbone:pTWIST ENTRY; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37248633
Proper citation: RRID:Addgene_193536 Copy
Species: Homo sapiens
Genetic Insert: Ribophorin I
Vector Backbone Description: Vector Backbone:pcDNA4/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34879065
Proper citation: RRID:Addgene_185351 Copy
Genetic Insert: P-R-lox-TT-lox-bla
Vector Backbone Description: Vector Backbone:E. coli K-12 MG1655; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:36823420
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_188474 Copy
Genetic Insert: P-R-lox-TT-lox-knt
Vector Backbone Description: Vector Backbone:E. coli K-12 MG1655; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:36823420
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_188475 Copy
Genetic Insert: P*-R-lox-TT-lox-knt
Vector Backbone Description: Vector Backbone:E. coli K-12 MG1655; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:36823420
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.06.10.495621v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_188476 Copy
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