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Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:the genome of E1/E3 deleted human adenovirus 5; Vector Types:Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30793964
Comments: This plasmid contains an in frame deletion of R346 and R347 from the L2 pV open reading frame. This mutation is not expected to affect its function.
Proper citation: RRID:Addgene_122699 Copy
Species: Synthetic
Genetic Insert: MS2-NLS, codon-optimised for Arabidopsis
Vector Backbone Description: Backbone Size:2149; Vector Backbone:pMK-RQ; Vector Types:Golden Gate compatible cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31557222
Comments: Plasmid is designed for Golden Gate cloning using the enzyme BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 380-385p
MS2-NLS coding sequence = 393-872bp
BsaI site #2 = 878-883bp
Proper citation: RRID:Addgene_122837 Copy
Species: Homo sapiens
Genetic Insert: SHP-2CS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3500; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15133037
Proper citation: RRID:Addgene_12284 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:0; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15155912
Proper citation: RRID:Addgene_1229 Copy
Genetic Insert: CfaN
Vector Backbone Description: Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26854538
Comments: Note: Plasmid contains a 5xHis tag rather than 6xHis tag.
Proper citation: RRID:Addgene_122949 Copy
Species: Homo sapiens
Genetic Insert: STAT1 Y701F
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. Y701F mutation made using QuikChange mutagenesis kit. The STAT1 insert contains a D372N mutation compared to all available reference sequences.
Proper citation: RRID:Addgene_12302 Copy
Species: Homo sapiens
Genetic Insert: STAT1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. The hSTAT1 insert contains an N372D mutation compared to all available reference sequences.
Proper citation: RRID:Addgene_12301 Copy
Genetic Insert: EGFP-VAMP3-GST
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30704911
Proper citation: RRID:Addgene_122971 Copy
Species: Homo sapiens
Genetic Insert: STAT1 S727E
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. S727E mutation made using QuikChange mutagenesis kit. STAT1 also contains N372D mutation compared to all available reference sequences.
Proper citation: RRID:Addgene_12305 Copy
Species: Homo sapiens
Genetic Insert: STAT1 S727A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. S727A mutation made using QuikChange mutagenesis kit.
Proper citation: RRID:Addgene_12304 Copy
Species: Danio rerio
Genetic Insert: Fgf2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16862139
Proper citation: RRID:Addgene_12309 Copy
Species: Synthetic
Genetic Insert: 2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and TEV HcPro (silencing suppressor) expression vector for plants. Introns avoid expression of fLUC in Agrobacteria. Increased virulence in Agro. through VirGN54D in the vector backbone
Proper citation: RRID:Addgene_123193 Copy
Species: Synthetic
Genetic Insert: 2x35S::ZCT1::tMAS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Vector for transient expression of ZCT1 from Chatharanthus roseus. ZCT1 is a repressor of the C. roseus STR1 promoter. Can be used in combination with pSB137 for transactivation/repression assays.
Proper citation: RRID:Addgene_123195 Copy
Species: Synthetic
Genetic Insert: pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and rLUC expression vector for plants. Introns avoid expression in Agrobacteria. Increased virulence in Agro. through VirGN54D in the vector backbone.
Proper citation: RRID:Addgene_123194 Copy
Species: Synthetic
Genetic Insert: 2x35S::GUS::tMAS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Vector for transient expression of GUS (contains two introns for avoiding expression in Agrobacteria). Small insert size and VirGN54D in the vector backbone facilitate strong transient expression in plants.
Proper citation: RRID:Addgene_123197 Copy
Species: Synthetic
Genetic Insert: tMAS::rLUC-I::pMAS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Vector for transient expression of a Renilla luciferase (rLUC, intron for avoiding expression in Agrobacteria). Small insert size and VirGN54D in the vector backbone facilitate strong transient expression in plants.
Proper citation: RRID:Addgene_123199 Copy
Species: Synthetic
Genetic Insert: 2x35S::P19::tNOS 2x35S::fLUC-I::tNOS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and TBSV P19 (silencing suppressor) expression vector for plants. Introns avoid expression of fLUC in Agrobacteria. Increased virulence in Agrob. through VirGN54D in the vector backbone
Proper citation: RRID:Addgene_123191 Copy
Species: Synthetic
Genetic Insert: 2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5851; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC and turboGFP expression vector for plants. Introns avoid expression in Agrobacteria. Increased virulence in Agrobacteria through VirGN54D in the vector backbone.
Proper citation: RRID:Addgene_123190 Copy
Species: Synthetic
Genetic Insert: 2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
Vector Backbone Description: Backbone Marker:Modified from pAGM4723 (Weber et al., 2011; DOI:10.1371/journal.pone.0016765) by the addition of the VirGN54D gene; Backbone Size:5874; Vector Backbone:pSB90; Vector Types:Plant Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Transient fLUC expression vector for plants; Intron avoids expression in Agrobacteria; Increased virulence in Agrobacteria through VirGN54D in the vector backbone.
Proper citation: RRID:Addgene_123188 Copy
Species: Agrobacterium tumefaciens
Genetic Insert: VirGN54D from pAD1289 (a gift from A. Das)
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:12773; Vector Backbone:pAGM4723; Vector Types:Plant Expression, Level 2 acceptor vector for expression in plants ; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo; Weber et al., 2011) level 2 acceptor vector with Mutation VirGN54D for increased virulence. Increased virulence in Agrobacteria for a high-efficiency gene transfer (increased with and without acetosyringone, compared to original vector control).
Proper citation: RRID:Addgene_123187 Copy
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