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Species: Synthetic
Genetic Insert: Pc promoter - mTurquoise2
Vector Backbone Description: Backbone Marker:obtained from Culture Collection of Switzerland; Backbone Size:8248; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_108235 Copy
Species:
Genetic Insert: F' Tra operon
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt.
Proper citation: RRID:Addgene_12082 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pCAP05; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Tetracycline
References:
Comments: pCAP05 can be linearized through the restriction sites ClaI and XhoI. Linear vector can be used for the construction of cluster-specific capture vectors using the same methodology as described for pCAP03.
Proper citation: RRID:Addgene_102920 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2026; Vector Backbone:pRH144; Vector Types:mini-transposon system suitable for sequencing, shuttling and gene fusions; Bacterial Resistance:Tetracycline
References:
Comments: Artificial DNA for plasmid pMin1
GenBank: Z50148.1
Proper citation: RRID:Addgene_102959 Copy
Species: M. barkeri
Genetic Insert: tRNA synthetase
Vector Backbone Description: Backbone Size:6290; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_49086 Copy
Species: Xanthamonas
Genetic Insert: NS3
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
References:
Comments: Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_50611 Copy
Species: Xanthamonas
Genetic Insert: NS9
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
References:
Comments: Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_50617 Copy
Species:
Genetic Insert: E. coli Conjugation genes
Vector Backbone Description: Vector Backbone:RK24; Vector Types:; Bacterial Resistance:Tetracycline
References:
Comments: LB Lennox with Tetracycline to maintain plasmid, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media.
Proper citation: RRID:Addgene_51950 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_59399 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_59398 Copy
Species:
Genetic Insert: rfp
Vector Backbone Description: Backbone Size:7569; Vector Backbone:pCM62; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_59775 Copy
Species: Escherichia coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_55750 Copy
Species: Synthetic
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:pFG018; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_62817 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: AUG --> UUG start codon in lacZ
Proper citation: RRID:Addgene_63861 Copy
Species: Other
Genetic Insert: tdh1
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:cloning vector; Bacterial Resistance:Tetracycline
References:
Comments: Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1 cloned into the PstI site of pBR322.
Proper citation: RRID:Addgene_66158 Copy
Species: Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110)
Genetic Insert: B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
Vector Backbone Description: Vector Backbone:pSUP202pol4; Vector Types:Conjugative bacterial vector; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_67032 Copy
Species: Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110)
Genetic Insert: B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
Vector Backbone Description: Vector Backbone:pSUP202pol4; Vector Types:Conjugative bacterial vector; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_67030 Copy
Species: Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110)
Genetic Insert: B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
Vector Backbone Description: Vector Backbone:pSUP202pol4; Vector Types:Conjugative bacterial vector; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_67035 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a -1 frameshift mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63875 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: This plasmid carries a UAG nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63870 Copy
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