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Species: Haematococcus pluvialis
Genetic Insert: crtO
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9259; Vector Backbone:pAC-BETAipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17634749
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53301 Copy
Species: Erwinia herbicola Eho10
Genetic Insert: crtE, crtB
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53300 Copy
Species: Erwinia herbicola Eho10
Genetic Insert: crtE, idi, crtX, crtY, crtI, crtB, crtZ
Vector Backbone Description: Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:7919981
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53262 Copy
Species: E. coli
Genetic Insert: etpC
Vector Backbone Description: Backbone Size:3719; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, pACYC184; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19707189
Comments: Please note that there is a L to M mutation in etpC. This mutation should not affect function.
Proper citation: RRID:Addgene_53533 Copy
Species: e. coli
Genetic Insert: pLux
Vector Backbone Description: Backbone Size:1000; Vector Backbone:pLuxR; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24104480
Proper citation: RRID:Addgene_53544 Copy
Genetic Insert: None
Vector Backbone Description: Vector Backbone:pCAndy; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24550272
Proper citation: RRID:Addgene_53571 Copy
Species: Other
Genetic Insert: λCI-β-flap fusion
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:9121589
Comments: Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details.
This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53733 Copy
Species: Synthetic
Genetic Insert: Repressor 4F2 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73431 Copy
Species: Synthetic
Genetic Insert: Repressor 1B6 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73430 Copy
Species: Synthetic
Genetic Insert: Repressor C4 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73427 Copy
Species: Synthetic
Genetic Insert: Repressor 1E4 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73436 Copy
Species: Synthetic
Genetic Insert: Repressor 3A2 (orthogonal T7-lac repressor)
Vector Backbone Description: Backbone Marker:Luciano Marraffini (Addgene plasmid # 46569); Backbone Size:9296; Vector Backbone:pdCas9; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27079979
Comments: Also encodes dCas9 and tracrRNA.
Proper citation: RRID:Addgene_73435 Copy
Species: Synthetic
Genetic Insert: pAzFRS.2.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Comments: Note: This plasmid exists as a tandem dimer approximately 12 kb in length. Please see the diagnostic digest in the Resource Information section.
Proper citation: RRID:Addgene_73546 Copy
Species: Synthetic
Genetic Insert: pAcFRS.1.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73545 Copy
Species: Synthetic
Genetic Insert: pAcFRS.2.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73544 Copy
Species: Synthetic
Genetic Insert: pAzFRS.1.t1
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26571098
Proper citation: RRID:Addgene_73547 Copy
Species: Synthetic
Genetic Insert: Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
Vector Backbone Description: Backbone Marker:Terrence Lai (this study); Backbone Size:1878; Vector Backbone:colE1 origin and chloramphenicol resistance; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74091 Copy
Species: E. coli MG1655
Genetic Insert: lldR
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74095 Copy
Species: E. coli MG1655
Genetic Insert: Bba_K822000 (L-Lactate promoter - E. coli MG1655)
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Proper citation: RRID:Addgene_74094 Copy
Species: Synthetic
Genetic Insert: TL9 RBS
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2044; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716
Comments: This plasmid contains the TL9 RBS upstream of GFP.
Proper citation: RRID:Addgene_74098 Copy
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