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Species: Escherichia coli
Genetic Insert: TurboID with 3XHA tag
Vector Backbone Description: Backbone Marker:Engler et al. 2014; Vector Backbone:pAGM41258; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35619173
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.01.433373v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172795 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173451 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y718E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173450 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y715E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173448 Copy
Species: Escherichia coli
Genetic Insert: E. coli E69 (O9a:K30) Wzc_K540M with Y713E
Vector Backbone Description: Backbone Size:4542; Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34272394
Proper citation: RRID:Addgene_173447 Copy
Species: Escherichia coli
Genetic Insert: Tus
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5240; Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34161082
Comments: The plasmid backbone is originally pET24b-APEX2 (Plasmid #111702) acquired from Addgene. In this submission, the Apex2 insert has been replaced with NusA-Tus.
Proper citation: RRID:Addgene_165959 Copy
Species: Escherichia coli
Genetic Insert: lacI
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Proper citation: RRID:Addgene_169243 Copy
Species: Escherichia coli
Genetic Insert: rhaRS
Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the 2bp deletion in rTBC0541 (6796bp) as compared to the depositor's sequence does not affect plasmid function.
Proper citation: RRID:Addgene_169244 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167599 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167597 Copy
Species: Escherichia coli
Genetic Insert: trpC-tonB
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2546922
Comments: Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167591 Copy
Species: Escherichia coli
Genetic Insert: tonB fs
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: The frame-shift of the TonB amphipathic helix was produced by deletion of bp 607 (G) and insertion of a new bp (T) following position 636 of the subsequent tonB open reading frame. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167593 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167603 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167602 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167601 Copy
Species: Escherichia coli
Genetic Insert: ExbB, ExbD
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_167605 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, R204C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167620 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, H20A, N208C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167625 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, N208C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167624 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, H20A, R212C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167629 Copy
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