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Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 97-132
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52150 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 133-168
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52157 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 133-168
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52158 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 133-168
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52155 Copy
Species: Homo sapiens
Genetic Insert: D43951
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52156 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 133-168
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52153 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 133-168
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52154 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 133-168
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52151 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 133-168
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52152 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 169-204
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52159 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 241-283
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52167 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 205-240
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52164 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 205-240
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52165 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 169-204
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52162 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 284-319
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52171 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 284-319
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52172 Copy
Species: Homo sapiens
Genetic Insert: Pumilio homology domain amino acids 284-319
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2280; Vector Backbone:pChlr-pNEB193; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24581042
Proper citation: RRID:Addgene_52173 Copy
Species: Synthetic
Genetic Insert: PRHIII-sfGFP
Vector Backbone Description: Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24788326
Comments: Plasmid is designed for use in Geobacillus stearothermophilus NUB3621 (as well as E. coli.) Construct is described in the article linked at the bottom of this page. This plasmid is a companion to pIM1708-PsurP-surT-agaN-pNW33N which was previously submitted. As with pIM1708, despite the fact that your plasmid map displays an ampicillin resistance gene, the correct antibiotic resistance gene is a chloramphenicol acetyltransferase. We do not know if the ampicillin resistance gene is functional.
Proper citation: RRID:Addgene_52217 Copy
Species: Synthetic
Genetic Insert: dSpRY
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41277686
Proper citation: RRID:Addgene_240208 Copy
Vector Backbone Description: Vector Backbone:DP6; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41980963
Comments: Contains CcdB Dropout Cassette. Take care to grow on a resistant strain for plasmid amplification. For ease of use when constructing oligo libraries for RAMPhaGE Libraries, please find the corresponding RAMPhaGE Oligo Tool Colab Notebook: https://colab.research.google.com/github/Niopek-Lab/POGO_PANCE/blob/main/scripts/RAMPhaGE_Oligo_Tool.ipynb.
Please visit https://doi.org/10.1101/2025.06.12.659331 for bioRxiv preprint.
Proper citation: RRID:Addgene_242157 Copy
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