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Vector Backbone Description: Backbone Size:9626; Vector Backbone:All-in-One_Nickase-D10A; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27079678
Comments: Puromycin resistance gene is only for transient expression along with Cas9 nickase. Thus, drug selection period should be tested for each experiment. The presence of cellular puromycin resistance could be monitored by performing a control transfection with an empty backbone vector of either AIO-GFP or AIO-mCherry in which GFP/mCherry expression level represents puromycin resistance level.
Proper citation: RRID:Addgene_74630 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Janke et al., (2004) Yeast 21:947-962; Backbone Size:4940; Vector Backbone:pYM27; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26994102
Comments: During verification of the constructed cassettes by restriction analysis and sequencing we found out that there are 254 bp missing after the BamHI site in between the fluorophore and the Ashbya gossypii TEF promotor region regulating expression of the selectable marker in pFM571, pIM572 and pIM573. We checked the original pYM27 plasmid (Janke, et al., 2004, Yeast 21:947-962 ) we had in our hands and compared the obtained sequence to the published one. We also checked by restriction analysis pYM27 from an independent source (but also obtained from Euroscarf) and got the same results. In the published pYM27 sequence, there is a sequence AGATCC resembling BamHI site (GGATCC) at position 1044-1049 bp. Likely due to a BamHI star activity that led to cutting this BamHI-like site in parallel with the true BamHI site, the nucleotides between these two sites got lost during construction of pYM27 or its predecessors and are missing in pYM27 and thus also in our pYM27-based modules - pFM571, pIM572 and pIM573. It seems, however, that the functionality of the vector and of the selection marker is not disturbed since transformants were readily obtained with all the concerned cassettes when selecting on geneticin.
Proper citation: RRID:Addgene_74634 Copy
Species: Homo sapiens
Genetic Insert: human dynein light intermediate chain 1 amino acids 389-523
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25272277
Proper citation: RRID:Addgene_74599 Copy
Genetic Insert: yTagRFP-T
Vector Backbone Description: Backbone Marker:Marian Farkasovsky; Backbone Size:4522; Vector Backbone:pFM699; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26994102
Proper citation: RRID:Addgene_74639 Copy
Genetic Insert: yTagRFP-T
Vector Backbone Description: Backbone Marker:Marian Farkasovsky; Backbone Size:4905; Vector Backbone:pFM689; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26994102
Comments: Compared to the published sequence of the pFA6a-hphNT1 backbone vector (Janke, et al., 2004, Yeast 21: 947-962) we found one PstI site missing in its sequence. It is the site in the vector –promoter part and there is a substitution of A by G in position 284 bp that arose most probably during construction of preceding cassettes, e.g. pFA6-hphMX4. We introduced the substitution also into maps of our modules carrying the hygromycin resistance - pFM689, pIM690 and pIM691.
Proper citation: RRID:Addgene_74638 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Janke et al., (2004) Yeast 21:947-962; Backbone Size:3784; Vector Backbone:pFA6a-natNT2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26994102
Proper citation: RRID:Addgene_74636 Copy
Species: Homo sapiens
Genetic Insert: CSK
Vector Backbone Description: Backbone Marker:this lab, modified from pcDNA3; Vector Backbone:pcFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23707526
Comments: The depositor notes that a BamHI restriction site was inserted following the start codon of CSK. This site does not affect plasmid function.
Proper citation: RRID:Addgene_74503 Copy
Species: Danio rerio
Genetic Insert: cmlc2::mCherry
Vector Backbone Description: Backbone Size:3048; Vector Backbone:pKHR4; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27003937
Proper citation: RRID:Addgene_74624 Copy
Species: Homo sapiens
Genetic Insert: CSK
Vector Backbone Description: Backbone Marker:this lab, modified from pcDNA3; Vector Backbone:pcFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23707526
Comments: The depositor notes that a BamHI restriction site was inserted following the start codon of CSK. This site does not affect plasmid function.
Proper citation: RRID:Addgene_74502 Copy
Species: Homo sapiens
Genetic Insert: Grb10
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23246379
Proper citation: RRID:Addgene_74501 Copy
Species: Mus musculus
Genetic Insert: IL2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5060; Vector Backbone:pCR3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11198480
Proper citation: RRID:Addgene_74467 Copy
Species: Homo sapiens
Genetic Insert: CSK
Vector Backbone Description: Backbone Marker:this lab, modified from pcDNA3; Vector Backbone:pcFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23707526
Comments: The depositor notes that a BamHI restriction site was inserted following the start codon of CSK. This site does not affect plasmid function.
Proper citation: RRID:Addgene_74505 Copy
Species: Homo sapiens
Genetic Insert: CSK
Vector Backbone Description: Backbone Marker:this lab, modified from pcDNA3; Vector Backbone:pcFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23707526
Proper citation: RRID:Addgene_74504 Copy
Species: Danio rerio
Genetic Insert: FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
Vector Backbone Description: Backbone Size:3048; Vector Backbone:pKHR4; Vector Types:Cre/Lox, Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27003937
Proper citation: RRID:Addgene_74625 Copy
Species: Homo sapiens
Genetic Insert: HOXB3
Vector Backbone Description: Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26482852
Proper citation: RRID:Addgene_74508 Copy
Species: Homo sapiens
Genetic Insert: Neuropeptide Y
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:GFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_74629 Copy
Species: synthetic
Genetic Insert: TEV- 2xV5 tag (short version)
Vector Backbone Description: Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27172193
Comments: We observe rather poor growth of bacteria with the pJet1.2 backbone, resulting in low yields of the plasmid preps.
Proper citation: RRID:Addgene_74774 Copy
Species: Homo sapiens
Genetic Insert: BRD1A
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|11321642
Proper citation: RRID:Addgene_74655 Copy
Species: Homo sapiens
Genetic Insert: JARID1C
Vector Backbone Description: Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25712104
Comments: GFP is driven by a separate promoter (UBC promoter), and is used only to track cells and the efficiency of infection/transfection.
Proper citation: RRID:Addgene_74776 Copy
Species: Homo sapiens
Genetic Insert: STK10
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. gi|5174701
Proper citation: RRID:Addgene_74659 Copy
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