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Genetic Insert: AtCAS9
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original AtCas9 pDe-CAS9 vector from H. Puchta lab.
Proper citation: RRID:Addgene_112070 Copy
Species: Homo sapiens
Genetic Insert: Rad
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115241
Comments: The C249 mutant contains a deletion of the calmodulin binding site and all C-terminal in vitro phosphorylation sites of Rad. Localizes mainly to cytosol in C2C12 cells (displaced from membrane).
Proper citation: RRID:Addgene_11215 Copy
Genetic Insert: iGABASnFR.F102Y.Y137L
Vector Backbone Description: Vector Backbone:pAAV.SynFLEX; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112171 Copy
Species: Homo sapiens
Genetic Insert: ACTB
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30061719
Comments: 1 copy of Riboglow (variant A), flanked by restriction sites as follows: KpnI - A - BamHI
Proper citation: RRID:Addgene_112055 Copy
Species: Homo sapiens
Genetic Insert: IGF-1R
Vector Backbone Description: Backbone Size:4888; Vector Backbone:pBabe-bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15247278
Comments: The insert was cloned from pcDNA IGF-1R (which contains a 4.2kb IGF-1R insert that lacks about 700bp of 3' sequence. This insert was cloned into the XhoI-XbaI sites of pcDNA). An EcoRI/XbaI fragment of pcDNA-IGF-1R was then inserted into SnaBI site of pBABE-bleo (EcoRI and XbaI sites were blunted).
Proper citation: RRID:Addgene_11212 Copy
Species: Homo sapiens
Genetic Insert: U1
Vector Backbone Description: Backbone Marker: "Reduction of Target Gene Expression by a Modified U1 snRNA" (Beckley et al, 2001, Mol Cell Bio); Vector Backbone:U1 human; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30061719
Comments: In partial sequence, 1 copy of Riboglow (truncated version A)--U1 sequence is in lower case letters, Riboglow sequence is in upper case letters
Proper citation: RRID:Addgene_112059 Copy
Species: Homo sapiens
Genetic Insert: ACTB
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30061719
Comments: 3 copies of Riboglow (variant A), separated by restriction sites as follows: KpnI - A - KpnI - A - BamHI - A - BamHI
Proper citation: RRID:Addgene_112057 Copy
Species: Homo sapiens
Genetic Insert: ACTB
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30061719
Comments: 4 copies of Riboglow (variant A), separated by restriction sites as follows: KpnI - A - KpnI - A - BamHI - A - BamHI - A - BamHI
Proper citation: RRID:Addgene_112058 Copy
Genetic Insert: iGABASnFR.R205A
Vector Backbone Description: Vector Backbone:pAAV.hSynapsin; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112162 Copy
Genetic Insert: iGABASnFR.F102Y.Y137L
Vector Backbone Description: Vector Backbone:pAAV.SynFLEX; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112165 Copy
Genetic Insert: iGABASnFR
Vector Backbone Description: Vector Backbone:pAAV.CAG-FLEX; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112166 Copy
Genetic Insert: iGABASnFR
Vector Backbone Description: Vector Backbone:pAAV.SynFLEX; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112163 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SWP1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112048 Copy
Genetic Insert: iGABASnFR.F102G
Vector Backbone Description: Vector Backbone:pAAV.CAG-FLEX; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112167 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SCT1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29348368
Proper citation: RRID:Addgene_112047 Copy
Genetic Insert: iGABASnFR.F102Y.Y137L
Vector Backbone Description: Vector Backbone:pAAV.CAG-FLEX; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112168 Copy
Vector Backbone Description: Backbone Size:2573; Vector Backbone:peDK.11; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29724956
Proper citation: RRID:Addgene_112150 Copy
Genetic Insert: iGABASnFR
Vector Backbone Description: Vector Backbone:pAAV.hSynapsin; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31308547
Comments: Please visit https://www.biorxiv.org/content/early/2018/05/14/322578 for bioRxiv preprint.
Proper citation: RRID:Addgene_112159 Copy
Species: Synthetic
Genetic Insert: anti-GFP FLIPPER-body
Vector Backbone Description: Backbone Size:5371; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29327239
Proper citation: RRID:Addgene_112157 Copy
Species: Rattus norvegicus
Genetic Insert: Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15229221
Proper citation: RRID:Addgene_112144 Copy
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