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Species: Homo sapiens
Genetic Insert: PLCdelta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9512420
Comments: This construct encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids (Stauffer TP, Ahn S, Meyer T; Curr Biol. 1998 8:343-6). It is best used in combination with a second fluorescent protein that uniformly marks the plasma membrane (the last 20 amino acids "CAAX" region of KRas is often used as a PM marker). Relative changes in cell-wide or local plasma membrane PI(4,5)P2 can be determined by using a mask for the PM region and by dividing the two background subtracted PH domain and CAAX images. At high expression levels, this construct interferes with PI(4,5)P2 signaling and alters cortical actin (Raucher D et al Cell. 2000 100:221-8).
Proper citation: RRID:Addgene_21179 Copy
Species: Rattus norvegicus
Genetic Insert: CAMKIIalpha-T286A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10102820
Proper citation: RRID:Addgene_21220 Copy
Species: Rattus norvegicus
Genetic Insert: CAMKIIalpha
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4725; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9768845
Proper citation: RRID:Addgene_21226 Copy
Species: Rattus norvegicus
Genetic Insert: CaMKIIbeta(A303R)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10102820
Proper citation: RRID:Addgene_21224 Copy
Species: Mus musculus
Genetic Insert: Axin2 promoter
Vector Backbone Description: Backbone Marker:Clontech (discontinued); Backbone Size:4300; Vector Backbone:d2EGFP-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11809808
Comments: The 5.6-kb DNA fragment was excised from Axin2-Luciferase using KpnI and HindIII (the HindIII site was filled in with Klenow DNA polymerase) and cloned into the KpnI- and SmaI-digested pd2EGFP-1 vector.
Proper citation: RRID:Addgene_21276 Copy
Species: Caenorhabditis elegans
Genetic Insert: cye-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21392 Copy
Species: Caenorhabditis elegans
Genetic Insert: L3787 promoter::GFP in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21377 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-11 promoter (no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21385 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 promoter (short, no ATG) in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21384 Copy
Species: Caenorhabditis elegans
Genetic Insert: gld-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21389 Copy
Species: Homo sapiens
Genetic Insert: PLCdelta
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4701; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11134066
Proper citation: RRID:Addgene_21262 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-56 promoter in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21383 Copy
Species: Caenorhabditis elegans
Genetic Insert: him-3 prom in PDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:PDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21382 Copy
Species: Caenorhabditis elegans
Genetic Insert: lip-1 M1M2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_21381 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:300; Vector Backbone:R6K origin PCR fragment; Vector Types:Worm Expression, Adds unc-119 marker to Amp resistant plasmids; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19520111
Comments: This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
Proper citation: RRID:Addgene_21595 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::citrine(w introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21511 Copy
Species: Homo sapiens
Genetic Insert: HIF1AN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12615973
Proper citation: RRID:Addgene_21403 Copy
Species: Homo sapiens
Genetic Insert: ALIX
Vector Backbone Description: Backbone Size:4700; Vector Backbone:mCherry; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18948538
Proper citation: RRID:Addgene_21504 Copy
Species: Caenorhabditis elegans
Genetic Insert: (Gly)5Ala::GFP(w introns)::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21637852
Proper citation: RRID:Addgene_21509 Copy
Species: Mus musculus
Genetic Insert: GFP-ING2(pHD)
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12859901
Proper citation: RRID:Addgene_21589 Copy
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