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Species: Zea mays
Genetic Insert: G2-like transcription factor from EE295362
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/D vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27748 Copy
Species: Mus musculus
Genetic Insert: mouse myc ULK1 4SA
Vector Backbone Description: Backbone Marker:Addgene plasmid 17399; Backbone Size:9135; Vector Backbone:pQCXIN Neo dest; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205641
Proper citation: RRID:Addgene_27628 Copy
Species: Mus musculus
Genetic Insert: mouse myc ULK1 4SA
Vector Backbone Description: Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21205641
Proper citation: RRID:Addgene_27625 Copy
Species: Zea mays
Genetic Insert: MYB transcription factor from DR785526
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/D vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27747 Copy
Species: Zea mays
Genetic Insert: SBP transcription factor from DR821052
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/D vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27742 Copy
Species: Zea mays
Genetic Insert: ZmMYB42 transcription factor from AM156908
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/D vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27740 Copy
Species: Zea mays
Genetic Insert: ZmMYB95 (MYB-IF25) transcription factor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/D vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27741 Copy
Species: Zea mays
Genetic Insert: CPP transcription factor from EB166014
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/D vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27717 Copy
Species: Homo sapiens
Genetic Insert: cavin-3
Vector Backbone Description: Backbone Marker:Helenius lab; Backbone Size:5502; Vector Backbone:pHA-N-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20070607
Comments: The mutation R8P is a known natural variant of cavin-3 (http://www.uniprot.org/uniprot/Q969G5) The cavin-3 cDNA used in this plasmid originated from the ORFeome library.
Proper citation: RRID:Addgene_27711 Copy
Species: Mus musculus
Genetic Insert: Cortactin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Adapted from Natalie Soulet Cortactin-pEGFP-C1 construct.
Proper citation: RRID:Addgene_27676 Copy
Species: Mus musculus
Genetic Insert: OCRL1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 30533468 (MGC:93022).
Proper citation: RRID:Addgene_27675 Copy
Species: Rattus norvegicus
Genetic Insert: Ap2m1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5200; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21445324
Comments: Rat μ2 was cloned from a construct provided by David Owen (see Collins et al. 2002).
mCherry was inserted within μ2 at residue 236 with BglII and NdeI sites.
Proper citation: RRID:Addgene_27672 Copy
Species: Zea mays
Genetic Insert: MYB transcription factor from EE039390
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/D vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27727 Copy
Species: Homo sapiens
Genetic Insert: Dynamin 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 6473791 (MGC:57679).
Proper citation: RRID:Addgene_27689 Copy
Species: Mus musculus
Genetic Insert: Cofilin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Gene PCR from cDNA library from mouse brain
Proper citation: RRID:Addgene_27687 Copy
Species: Zea mays
Genetic Insert: C2C2-GATA transcription factor from DR807759
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25053252
Comments: This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org), and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/D vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
Proper citation: RRID:Addgene_27720 Copy
Species: Homo sapiens
Genetic Insert: FBP17
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from cDNA library from human kidney.
This plasmid may be slow growing in bacteria. For difficult constructs you can use TG1s (they are Endonuclease positive, but good for cloning) and INV F' E.coli strains. The latter give the best results. Also when growing in culture try to switch to 2xTY media, which is richer that LB.
Proper citation: RRID:Addgene_27688 Copy
Species: Mus musculus
Genetic Insert: CIP4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 3500118 (MGC:6673).
Proper citation: RRID:Addgene_27685 Copy
Species: Mus musculus
Genetic Insert: Ack1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 6400209 (MGC:63428).
Proper citation: RRID:Addgene_27684 Copy
Species: Homo sapiens
Genetic Insert: Arp3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21445324
Comments: Cloned from IMAGE clone: 5261640 (MGC:57216).
Proper citation: RRID:Addgene_27682 Copy
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