Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Vector Backbone Description: Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30185429
Comments: Clone cDNA into vector using a Gateway LR reaction. Transform verified plasmid into an expression strain (e.g. BL21(DE3)pLysS) for recombinant protein production.
Proper citation: RRID:Addgene_139787 Copy
Species: Homo sapiens
Genetic Insert: HOXA9
Vector Backbone Description: Vector Backbone:pFUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30530727
Proper citation: RRID:Addgene_139827 Copy
Species: Mus musculus
Genetic Insert: Myb
Vector Backbone Description: Vector Backbone:pFUW-tetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30530727
Comments: Depositor confirms these point mutations in Myb do not affect plasmid function.
Proper citation: RRID:Addgene_139826 Copy
Species: Mus musculus
Genetic Insert: SUMO1 GG
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17159996
Proper citation: RRID:Addgene_13990 Copy
Species: Gallus gallus
Genetic Insert: shh
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8269518
Proper citation: RRID:Addgene_13991 Copy
Species: Cricetulus griseus (Chinese hamster)
Genetic Insert: Mitotic Centromere Associated Kinesin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4709; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12446739
Proper citation: RRID:Addgene_13987 Copy
Genetic Insert: Lineage tracing library
Vector Backbone Description: Backbone Marker:Allon Klein; Vector Backbone:pLARRY-EGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31974159
Proper citation: RRID:Addgene_140024 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:7712; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31974159
Proper citation: RRID:Addgene_140025 Copy
Species: Methanosarcina mazei and Methanosarcina bakeri
Genetic Insert: AbKRS-chIPYE
Vector Backbone Description: Vector Backbone:pAS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37935196
Proper citation: RRID:Addgene_140020 Copy
Species: Synthetic
Genetic Insert: Enhanced Green Fluorescent Protein
Vector Backbone Description: Backbone Size:5721; Vector Backbone:Addgene #96930; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_140027 Copy
Species: Synthetic
Genetic Insert: Yellow fluorescent protein
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32382027
Comments: Please visit https://www.biorxiv.org/content/10.1101/684944v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_140035 Copy
Species: Mus musculus
Genetic Insert: tandem HRS FYVE domain
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10970851
Proper citation: RRID:Addgene_140047 Copy
Species: Other
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Marker:Penn Vector Core; Backbone Size:4405; Vector Backbone:AAV2/9.cTnT.PI.EGFP.RBG; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32146862
Proper citation: RRID:Addgene_139980 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139988 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Keith Joung (Addgene Plasmid 43861); Vector Backbone:JDS246; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139987 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139991 Copy
Species: Synthetic
Genetic Insert: human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
Vector Backbone Description: Backbone Marker:Ketih Joung; Vector Backbone:pRZ33 ; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139999 Copy
Species: Synthetic
Genetic Insert: human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
Vector Backbone Description: Backbone Marker:Kleinstiver Lab (Addgene ID 139987); Vector Backbone:RTW3027; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Proper citation: RRID:Addgene_139997 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
Vector Backbone Description: Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Comments: The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids.
Proper citation: RRID:Addgene_140002 Copy
Species: Synthetic
Genetic Insert: human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
Vector Backbone Description: Backbone Marker:David Liu (Addgene Plasmid 112101); Vector Backbone:pCMV_ABEmax_P2A_GFP; Vector Types:Mammalian Expression, CRISPR, in vitro transcription; T7 promoter; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32217751
Comments: The depositing laboratory has released the equivalent ABE8e versions of this enzyme, which they have found to show much higher editing efficiency. See https://www.addgene.org/browse/article/28228617/ for the plasmids.
Proper citation: RRID:Addgene_140003 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the PRECISE-TBI Resources search. From here you can search through a compilation of resources used by PRECISE-TBI and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that PRECISE-TBI has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on PRECISE-TBI then you can log in from here to get additional features in PRECISE-TBI such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into PRECISE-TBI you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within PRECISE-TBI that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.