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Species: E. coli
Genetic Insert: gusA
Vector Backbone Description: Vector Backbone:RP4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: GenBank accession number JQ895026
Multiple-cloning site (MCS) of pLMB51: GATTACCTCA GTCTAGAGCT CGAGAAGCTT GGATCCATGG TACCCTACA
Proper citation: RRID:Addgene_40083 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH9
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_40860 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH7
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_40858 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH5
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_40856 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH3
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_40854 Copy
Species: Xanthomonas oryzae
Genetic Insert: repeat NH1
Vector Backbone Description: Backbone Size:3013; Vector Backbone:pTC14; Vector Types:; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_40852 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:6878; Vector Backbone:pUC19; Vector Types:broad host range cloning vector; Bacterial Resistance:Tetracycline
References:
Comments: Vector is a broad host range vector (bhr) and is used in the Methylobacterium extorquens AM1. Also can be maintained in the a-proteo-bacteria Agrobacterium tumefaciens, Methylobacterium strains CM4 and DM4, and Rhodobacter sphaeroides, the b-proteobacterium Ralstonia eutropha and the c-proteobacteria Methylococcus capsulatus Bath and Pseudomonas aeruginosa (see linked article).
Proper citation: RRID:Addgene_45826 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:6925; Vector Backbone:pOSIP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Reference:
St-Pierre F, Cui L et al., "One-step cloning and chromosomal integration of DNA", ACS Synthetic Biology, http://pubs.acs.org/doi/abs/10.1021/sb400021j
Proper citation: RRID:Addgene_45988 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: does not contain lacI/
ERG1, ERG8, MVD1: S. cerevisiae/
idi: E. Coli
Proper citation: RRID:Addgene_17816 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:3002; Vector Backbone:BASIC_SEVA_5a9.10; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178936 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:3146; Vector Backbone:BASIC_SEVA_5a7_pKD46.10; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178937 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2349; Vector Backbone:BASIC_SEVA_5a6.10; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178934 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:3077; Vector Backbone:BASIC_SEVA_5a7.10; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178935 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:5291; Vector Backbone:BASIC_SEVA_5a5a.10; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178933 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pIT5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: pIT5_TL is designed to be integrated into the lambda attachment site, using the lambda integrase helper plasmid pInt-ts (Addgene # 66076).
Proper citation: RRID:Addgene_173768 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4224; Vector Backbone:pBTBXh-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function.
Proper citation: RRID:Addgene_26080 Copy
Species: Mus musculus
Genetic Insert: mFar1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: EJ Steen et al. Nature 2009 10.1038/nature08721
Proper citation: RRID:Addgene_24629 Copy
Species: E. coli
Genetic Insert: 'tesA
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: EJ Steen et al. 2009 Nature 10.1038/nature08721
Proper citation: RRID:Addgene_24625 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:17653; Vector Backbone:unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Selectable Marker: Tetracycline; 30 μg/mL in E.coli and 100 μg/mL in Pseudomonas aeruginosa
Proper citation: RRID:Addgene_113347 Copy
Species:
Genetic Insert: sacB
Vector Backbone Description: Vector Backbone:pDAI-SceI; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
References:
Comments: Addgene NGS found K43E and T318P within the SacB translation compared to the best match NCBI reference sequence (ACF93714.1). The depositing laboratory confirms that these mutations should not adversely affect plasmid function
Proper citation: RRID:Addgene_113635 Copy
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