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Species: Synthetic
Genetic Insert: NLSPUFa_VP64
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71881 Copy
Species: Synthetic
Genetic Insert: dCas9-mCBPHAT
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71887 Copy
Species: Synthetic
Genetic Insert: dCas9-VP64
Vector Backbone Description: Vector Backbone:pAC90-pmax-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26768771
Comments: Please visit http://casil.io for more information, updates, and protocols. For more information on Cheng Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/albert-cheng/
Proper citation: RRID:Addgene_71879 Copy
Species: Synthetic
Genetic Insert: hypothetical protein SYNGTS_3005
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72271 Copy
Species: Homo sapiens
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Marker:EMD Biosciences; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12218187
Proper citation: RRID:Addgene_72214 Copy
Species: Homo sapiens
Genetic Insert: Dcp2
Vector Backbone Description: Backbone Marker:EMD Biosciences; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12218187
Proper citation: RRID:Addgene_72215 Copy
Species: Homo sapiens
Genetic Insert: AB42
Vector Backbone Description: Backbone Marker:Waldo, et al. PMID 10404163 ; Backbone Size:6000; Vector Backbone:pET28(A+) based GFP folding reporter; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12079364
Proper citation: RRID:Addgene_72203 Copy
Species: Homo sapiens
Genetic Insert: SSRP1 Homology Arms
Vector Backbone Description: Backbone Size:5771; Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: The depositing laboratory notes that this clone grows slowly and the liquid culture may need to be incubated a little longer than usual. Importantly, they note that even though the clone grows slowly, the plasmid works in CRISPR experiments. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_72370 Copy
Species: Homo sapiens
Genetic Insert: phosphoribosyl pyrophosphate amidotransferase
Vector Backbone Description: Backbone Size:5310; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Synthetic gene
Proper citation: RRID:Addgene_72378 Copy
Species: Acetobacter aceti 1023
Genetic Insert: acyl-CoA thioesterase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72417 Copy
Species: Acetobacter aceti 1023
Genetic Insert: arylesterase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72418 Copy
Species: Homo sapiens
Genetic Insert: ZNF3 Homology Arms
Vector Backbone Description: Backbone Size:5771; Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_72362 Copy
Species: Homo sapiens
Genetic Insert: ZNF219 Homology Arms
Vector Backbone Description: Backbone Size:5771; Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Comments: Addgene's quality control sequencing finds a G to E amino acid residue substitution in the linker between ZNF219 and the 3xFlag. This residue change is not thought to affect plasmid function. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_72367 Copy
Species: Acetobacter aceti 1023
Genetic Insert: succinyl-CoA:acetate CoA-transferase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72403 Copy
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72318 Copy
Species: Synthetic
Genetic Insert: modified YFP
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72316 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72382 Copy
Species: Acetobacter aceti 1023
Genetic Insert: putative CoA-transferase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72425 Copy
Species: Synthetic
Genetic Insert: modified YFP
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72308 Copy
Vector Backbone Description: Vector Backbone:PMB1+ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26315440
Comments: T203Y mutant from sfGFP
Proper citation: RRID:Addgene_72320 Copy
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