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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAC-DELTA Resource Report Resource Website 1+ mentions |
RRID:Addgene_53273 | lcyE | Arabidopsis thaliana | Chloramphenicol | PMID:8798688 | For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. | Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9476; Vector Backbone:pAC-LYC; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:56 | 1 | |
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pAC-ZETA Resource Report Resource Website 1+ mentions |
RRID:Addgene_53316 | pds | Synechococcus PCC7942 | Chloramphenicol | PMID:7919981 | For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. | Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:8106; Vector Backbone:pAC-PHYT; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:56 | 1 | |
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pAC-85b Resource Report Resource Website 1+ mentions |
RRID:Addgene_53282 | crtE, crtY, crtI | Erwinia herbicola (Pantoea agglomerans) Eho10 | Chloramphenicol | PMID:17634749 | For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. | Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:56 | 4 | |
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pAC-PHYTipi Resource Report Resource Website 1+ mentions |
RRID:Addgene_53283 | crtE, idi, crtY, crtI, crtB | Erwinia herbicola Eho10 | Chloramphenicol | PMID:17634749 | For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. | Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:56 | 1 | |
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pAC-94N Resource Report Resource Website 1+ mentions |
RRID:Addgene_53281 | crtY, crtI, crtB | Erwinia herbicola Eho10 | Chloramphenicol | PMID:17634749 | For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. | Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10609; Vector Backbone:pAC-BETA; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:56 | 1 | |
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pAC-ZETAipi Resource Report Resource Website 1+ mentions |
RRID:Addgene_53284 | pds | Synechococcus PCC7942 | Chloramphenicol | PMID:17634749 | For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. | Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:56 | 1 | |
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pAC-CANTHipi Resource Report Resource Website 1+ mentions |
RRID:Addgene_53301 | crtO | Haematococcus pluvialis | Chloramphenicol | PMID:17634749 | For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. | Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9259; Vector Backbone:pAC-BETAipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:56 | 9 | |
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pAC-PHYT Resource Report Resource Website |
RRID:Addgene_53300 | crtE, crtB | Erwinia herbicola Eho10 | Chloramphenicol | PMID:7919981 | For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. | Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:56 | 0 | |
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pAC-EHER Resource Report Resource Website 1+ mentions |
RRID:Addgene_53262 | crtE, idi, crtX, crtY, crtI, crtB, crtZ | Erwinia herbicola Eho10 | Chloramphenicol | PMID:7919981 | For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. | Backbone Marker:New England BioLabs; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:56 | 1 | |
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pJL030 Resource Report Resource Website |
RRID:Addgene_53533 | etpC | E. coli | Chloramphenicol | PMID:19707189 | Please note that there is a L to M mutation in etpC. This mutation should not affect function. | Backbone Size:3719; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, pACYC184; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:58 | 0 | |
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pTuLys2CMR2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53544 | pLux | e. coli | Chloramphenicol | PMID:24104480 | Backbone Size:1000; Vector Backbone:pLuxR; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:58 | 1 | ||
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pCAndy-NBioTAP Resource Report Resource Website 1+ mentions |
RRID:Addgene_53571 | None | Chloramphenicol | PMID:24550272 | Vector Backbone:pCAndy; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:58 | 1 | |||
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pACλCI-β-flap (831-1057) Resource Report Resource Website 1+ mentions |
RRID:Addgene_53733 | λCI-β-flap fusion | Other | Chloramphenicol | PMID:9121589 | Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details. This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids: http://www.addgene.org/browse/article/8393/ The following articles can provide more information on using the bacterial 2-hybrid system: Dove, et al. 1997 PMID 9121589 Dove and Hochschild 1998 PMID 9499408 Deaconescu, et al. 2006 PMID 16469698 Deighan, et al. 2008 PMID 18832144 Kuznedelov, et al. 2002 PMID 11823642 | Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:07:00 | 1 | |
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pINIT_cat Resource Report Resource Website 1+ mentions |
RRID:Addgene_46858 | Chloramphenicol | PMID:21410291 | Backbone Marker:unknown; Backbone Size:4980; Vector Backbone:pBR322; Vector Types:sequencing vector; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:03 | 6 | ||||
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pAC-EsaR-EsaI Resource Report Resource Website |
RRID:Addgene_47660 | esaI-term | Chloramphenicol | PMID:23879176 | esaI is 636 bp and indicated as lowercase letters in the partial sequence (between BamHI and XhoI sites). See pAC-EsaR for lac promoter and esaR sequence information. | Backbone Size:4511; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:09 | 0 | ||
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pAC-EsaR-I70V/D91G Resource Report Resource Website |
RRID:Addgene_47644 | esaR-I70V/D91G | Chloramphenicol | PMID:23363022 | esaR-I70V/D91G (750 bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map. | Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | Changed Isoleucine 70 to Valine and Aspartic acid 91 to Glycine | 2026-08-29 01:06:09 | 0 | |
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pAC-EsaR-I70V/D91G/V220A Resource Report Resource Website |
RRID:Addgene_47647 | esaR-I70V/D91G/V220A | Chloramphenicol | PMID:23363022 | esaR-I70V/D91G/V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map. | Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | Changed Isoleucine 70 to Valine, Aspartic acid 91 to Glycine, and Valine 220 to Alanine | 2026-08-29 01:06:09 | 0 | |
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pAC-EsaR-V220A Resource Report Resource Website |
RRID:Addgene_47645 | esaR-V220A | Chloramphenicol | PMID:23363022 | esaR-V220A (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map. | Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | Changed Valine 220 to Alanine | 2026-08-29 01:06:09 | 0 | |
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pAC-EsaR-D91G Resource Report Resource Website |
RRID:Addgene_47646 | esaR-D91G | Chloramphenicol | PMID:23363022 | esaR-D91G (750bp, indicated as lowercase letters in the partial sequence) is located between KpnI and BamHI sites in pAC-EsaR in the same way as wt esaR in pAC-EsaR. Please see pAC-EsaR for lac promoter and terminator sequence information and plasmid map. | Backbone Size:3880; Vector Backbone:pAC-EsaR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | Changed Aspartic acid 91 to Glycine | 2026-08-29 01:06:09 | 0 | |
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pAK501 Resource Report Resource Website |
RRID:Addgene_48107 | Chloramphenicol | PMID:23995928 | Backbone Marker:Dieter Haas; Backbone Size:8270; Vector Backbone:pME6010; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-29 01:06:13 | 0 |
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