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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pSico Resource Report Resource Website 10+ mentions |
RRID:Addgene_11578 | Ampicillin | PMID:15240889 | pSico allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on http://web.mit.edu/jacks-lab/ (the Jacks Lab website) | Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 29 | |||
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pMN19 Resource Report Resource Website 1+ mentions |
RRID:Addgene_11572 | CaMV 35S enhancers | Spectinomycin | PMID:10759496 | Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article. Kanamycin gene for plant selection on MS media. Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l). The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration. | Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin | Addgene | 2026-10-17 02:33:27 | 1 | ||
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pDSG291 Resource Report Resource Website |
RRID:Addgene_115605 | regulated adhesin construct | Ampicillin | PMID:30033369 | Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:26 | 0 | |||
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pDSG287 Resource Report Resource Website |
RRID:Addgene_115606 | regulated adhesin construct | Ampicillin | PMID:30033369 | Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:26 | 0 | |||
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pSKI015 Resource Report Resource Website |
RRID:Addgene_11570 | CaMV 35S enhancers | Cauliflower mosaic virus | Ampicillin | PMID:10759496 | The CaMV 35S enhancers in these vectors correspond to nucleotides -417 to -86 relative to the transcription start. Recommended Agrobacterium strain is GV3101 pMP90RK. Host and helper plasmid markers are kanamycin, gentamycin and rifampicin resistance. Plasmid selection in E. coli and in A. tumefaciens is ampicillin resistance. Sites that leave the pBstKS+ sequences intact and cut only on one side between pBstKS+ and either left or right border can be used for plasmid rescue. Note that there are additional BssS1 sites not shown on the map. The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration. | Backbone Marker:Hayashi et al, 1992; Backbone Size:10138; Vector Backbone:pPCVICEn4HPT; Vector Types:Plant Expression, Activation tagging; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 0 | |
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pDSG360 Resource Report Resource Website 1+ mentions |
RRID:Addgene_115601 | regulated adhesin construct | Kanamycin | PMID:30033369 | Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:33:26 | 1 | |||
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pKIOct4-Halo-IRES-Bsd Resource Report Resource Website |
RRID:Addgene_115689 | OCT4-Halo-IRES-Bsd | Synthetic | Kanamycin | PMID:31556488 | Please visit https://www.biorxiv.org/content/early/2018/04/18/299073 for BioRxiv preprint | Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:33:27 | 0 | |
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pDSG289 Resource Report Resource Website |
RRID:Addgene_115608 | regulated adhesin construct | Ampicillin | PMID:30033369 | Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:26 | 0 | |||
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pGL001 Resource Report Resource Website |
RRID:Addgene_115674 | fliC promoter fused with GFP | Escherichia coli | Ampicillin | PMID:31285239 | Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 0 | ||
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pDC191 Resource Report Resource Website 1+ mentions |
RRID:Addgene_115792 | Synaptobrevin2 | Rattus norvegicus | Ampicillin | PMID:28772123 | C103S is an additional mutation compared to NCBI reference- This will not affect plasmid function | Backbone Marker:Novagen; Vector Backbone:pET31b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S28C and C103S (please see comments below) | Addgene | 2026-10-17 02:33:28 | 1 |
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pGMGUS Resource Report Resource Website 1+ mentions |
RRID:Addgene_115678 | gusA, aac(3)IV, tsr | Streptomyces | Apramycin | Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV | Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin | Addgene | 2026-10-17 02:33:27 | 2 | ||
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pKEW321-MbCas12a-RFP-GFP-array Resource Report Resource Website |
RRID:Addgene_115661 | GFP and RFP array for MbCas12 | Ampicillin | PMID:30190307 | Backbone Size:5000; Vector Backbone:ColE1/AmpR; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 0 | |||
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LV-Sox10MCS5-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_115783 | mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter | Mus musculus | Ampicillin | PMID:23507034 | Backbone Size:6378; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 1 | ||
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pcDNA 3.3 HA-hTSPAN12 Resource Report Resource Website |
RRID:Addgene_115784 | TSPAN12 | Homo sapiens | Ampicillin | PMID:28658627 | N-terminal HA tag has no substantial effect in TOPFlash reporter assays induced with Norrin, compared to untagged control | Backbone Marker:Invitrogen; Backbone Size:5407; Vector Backbone:pcDNA3.3 TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | multiple silent mutations introduce restriction sites to enable chimera construction | Addgene | 2026-10-17 02:33:27 | 0 |
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Pinducer20-BSD-scFv-sfGFP-DNMT3A1 Resource Report Resource Website |
RRID:Addgene_115780 | scFv-sfGFP-DNMT3A1 | Homo sapiens | Ampicillin | PMID:30097071 | Backbone Marker:Stephen Elledge; Backbone Size:11791; Vector Backbone:Pinducer 20; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 0 | ||
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pKEW189-MBP-TEV-AcrVA1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_115660 | AcrVA1 | Moraxella bovoculi | Ampicillin | PMID:30190307 | Backbone Marker:UC Berkeley; Backbone Size:5900; Vector Backbone:2CT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 1 | ||
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pKEW270-pTet-empty Resource Report Resource Website |
RRID:Addgene_115669 | Ampicillin | PMID:30190307 | Backbone Marker:N/A; Backbone Size:3000; Vector Backbone:ColE1/AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 0 | ||||
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PB-CAG-GFPd2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_115665 | GFPd2 | Synthetic | Ampicillin | PMID:30794383 | Co-transfection with super piggybac transposase leads to stable integration in mammalian chromosomal DNA. | Backbone Size:5551; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 4 | |
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pcDNA 3.3 ss-V5-hFZD4 (aa 37-537) Resource Report Resource Website |
RRID:Addgene_115787 | FZD4 | Homo sapiens | Ampicillin | PMID:28658627 | N-terminal V5 tag has no substantial effect in TOPFlash reporter assays induced with Norrin, compared to untagged control. TOPFlash reporter assay can be inhibited by transfection of too much of this plasmid (see Lai et al., 2017 for detailed description of transfection conditions). | Backbone Size:5407; Vector Backbone:pcDNA3.3 TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:33:27 | 0 | |
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Rag2 T490A Resource Report Resource Website |
RRID:Addgene_115650 | Rag2 | Mus musculus | Ampicillin | PMID:21149691 | Vector Backbone:pLEXm; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | T490A | Addgene | 2026-10-17 02:33:27 | 0 |
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