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http://www.addgene.org/11578

Vector Backbone Description: Backbone Size:7566; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15240889
Comments: pSico allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on http://web.mit.edu/jacks-lab/ (the Jacks Lab website)

Proper citation: RRID:Addgene_11578 Copy   

•   Source: Addgene
  

http://www.addgene.org/11572

Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hajdukiewicz et al 1994; Backbone Size:11073; Vector Backbone:pPZP212; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10759496
Comments: Used to confirm activation-tagged genes. Contains multiple cloning sites adjacent to the same tetramerized CaMV 35S enhancers as in the activation-tagging vectors from this article. Kanamycin gene for plant selection on MS media. Plasmid selection in E. coli and in A. tumefaciens is spectinomycin (100 mg/l). The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.

Proper citation: RRID:Addgene_11572 Copy   

•   Source: Addgene
  

http://www.addgene.org/115605

Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033369

Proper citation: RRID:Addgene_115605 Copy   

•   Source: Addgene
  

http://www.addgene.org/115606

Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033369

Proper citation: RRID:Addgene_115606 Copy   

•   Source: Addgene
  

http://www.addgene.org/11570

Species: Cauliflower mosaic virus
Genetic Insert: CaMV 35S enhancers
Vector Backbone Description: Backbone Marker:Hayashi et al, 1992; Backbone Size:10138; Vector Backbone:pPCVICEn4HPT; Vector Types:Plant Expression, Activation tagging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10759496
Comments: The CaMV 35S enhancers in these vectors correspond to nucleotides -417 to -86 relative to the transcription start. Recommended Agrobacterium strain is GV3101 pMP90RK. Host and helper plasmid markers are kanamycin, gentamycin and rifampicin resistance. Plasmid selection in E. coli and in A. tumefaciens is ampicillin resistance. Sites that leave the pBstKS+ sequences intact and cut only on one side between pBstKS+ and either left or right border can be used for plasmid rescue. Note that there are additional BssS1 sites not shown on the map. The four enhancer repeats in the construct may be unstable in E. coli and Agrobacterium if stored at +4'C for extended time. Check by PCR with T7 (or M13-20) oligo and this one derived from the RB sequence: 5' acc cgc caa tat atc ctg 3'. This should give 1.4 kb band. Note that these oligos will not work in a transgenic plant, as the RB sequence is not transfered. Using this test, we found that after 1-2 weeks in a fridge the Agrobacterium strain loses on average one copy of a repeat, and after a month in a fridge there is only one copy left. It is a good idea to use a freshly streaked colony from a good -70'C glycerol stock for every infiltration.

Proper citation: RRID:Addgene_11570 Copy   

•   Source: Addgene
  

http://www.addgene.org/115601

Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB3K3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30033369

Proper citation: RRID:Addgene_115601 Copy   

•   Source: Addgene
  

http://www.addgene.org/115689

Species: Synthetic
Genetic Insert: OCT4-Halo-IRES-Bsd
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31556488
Comments: Please visit https://www.biorxiv.org/content/early/2018/04/18/299073 for BioRxiv preprint

Proper citation: RRID:Addgene_115689 Copy   

•   Source: Addgene
  

http://www.addgene.org/115608

Genetic Insert: regulated adhesin construct
Vector Backbone Description: Vector Backbone:pSB4A3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30033369

Proper citation: RRID:Addgene_115608 Copy   

•   Source: Addgene
  

http://www.addgene.org/115674

Species: Escherichia coli
Genetic Insert: fliC promoter fused with GFP
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31285239

Proper citation: RRID:Addgene_115674 Copy   

•   Source: Addgene
  

http://www.addgene.org/115792

Species: Rattus norvegicus
Genetic Insert: Synaptobrevin2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET31b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28772123
Comments: C103S is an additional mutation compared to NCBI reference- This will not affect plasmid function

Proper citation: RRID:Addgene_115792 Copy   

•   Source: Addgene
  

http://www.addgene.org/115678

Species: Streptomyces
Genetic Insert: gusA, aac(3)IV, tsr
Vector Backbone Description: Vector Backbone:pSG5; Vector Types:Gene knock out; Bacterial Resistance:Apramycin
Comments: Thermosensitive replication (< 37°C) in Streptomyces, Detection of single/double cross over by X-gluc colour reaction , aac(3)IV

Proper citation: RRID:Addgene_115678 Copy   

•   Source: Addgene
  

http://www.addgene.org/115661

Genetic Insert: GFP and RFP array for MbCas12
Vector Backbone Description: Backbone Size:5000; Vector Backbone:ColE1/AmpR; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190307

Proper citation: RRID:Addgene_115661 Copy   

•   Source: Addgene
  

http://www.addgene.org/115783

Species: Mus musculus
Genetic Insert: mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
Vector Backbone Description: Backbone Size:6378; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23507034

Proper citation: RRID:Addgene_115783 Copy   

•   Source: Addgene
  

http://www.addgene.org/115784

Species: Homo sapiens
Genetic Insert: TSPAN12
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5407; Vector Backbone:pcDNA3.3 TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28658627
Comments: N-terminal HA tag has no substantial effect in TOPFlash reporter assays induced with Norrin, compared to untagged control

Proper citation: RRID:Addgene_115784 Copy   

•   Source: Addgene
  

http://www.addgene.org/115780

Species: Homo sapiens
Genetic Insert: scFv-sfGFP-DNMT3A1
Vector Backbone Description: Backbone Marker:Stephen Elledge; Backbone Size:11791; Vector Backbone:Pinducer 20; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30097071

Proper citation: RRID:Addgene_115780 Copy   

•   Source: Addgene
  

http://www.addgene.org/115660

Species: Moraxella bovoculi
Genetic Insert: AcrVA1
Vector Backbone Description: Backbone Marker:UC Berkeley; Backbone Size:5900; Vector Backbone:2CT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190307

Proper citation: RRID:Addgene_115660 Copy   

•   Source: Addgene
  

http://www.addgene.org/115669

Vector Backbone Description: Backbone Marker:N/A; Backbone Size:3000; Vector Backbone:ColE1/AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190307

Proper citation: RRID:Addgene_115669 Copy   

•   Source: Addgene
  

http://www.addgene.org/115665

Species: Synthetic
Genetic Insert: GFPd2
Vector Backbone Description: Backbone Size:5551; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30794383
Comments: Co-transfection with super piggybac transposase leads to stable integration in mammalian chromosomal DNA.

Proper citation: RRID:Addgene_115665 Copy   

•   Source: Addgene
  

http://www.addgene.org/115787

Species: Homo sapiens
Genetic Insert: FZD4
Vector Backbone Description: Backbone Size:5407; Vector Backbone:pcDNA3.3 TOPO TA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28658627
Comments: N-terminal V5 tag has no substantial effect in TOPFlash reporter assays induced with Norrin, compared to untagged control. TOPFlash reporter assay can be inhibited by transfection of too much of this plasmid (see Lai et al., 2017 for detailed description of transfection conditions).

Proper citation: RRID:Addgene_115787 Copy   

•   Source: Addgene
  

http://www.addgene.org/115650

Species: Mus musculus
Genetic Insert: Rag2
Vector Backbone Description: Vector Backbone:pLEXm; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21149691

Proper citation: RRID:Addgene_115650 Copy   

•   Source: Addgene