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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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zebrafish similar to gpr151 (or gpcr-2037)_L (OZ535) Resource Report Resource Website |
RRID:Addgene_27212 | Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene zebrafish similar to gpr151 (or gpcr-2037)(see below). Please note that this plasmid does NOT contain the zebrafish similar to gpr151 (or gpcr-2037) sequence. Users must order the complementary plasmid zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536) [Addgene plasmid 27213] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GATGATGGC-3' in a bacterial two hybrid assay, and resulted in 12.54 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:44:10 | 0 | |
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pMIGR AMPK KD Resource Report Resource Website 1+ mentions |
RRID:Addgene_27296 | AMP Acticated Protein Kinase | Rattus norvegicus | Ampicillin | Backbone Size:6000; Vector Backbone:pMIGR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | Kinase Dead = Lys 45 to Arg | Addgene | 2026-10-17 02:44:11 | 2 | ||
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pAMPK alpha1 full length Resource Report Resource Website 1+ mentions |
RRID:Addgene_27297 | AMPK alpha1 | Mus musculus | Ampicillin | Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1/Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:44:11 | 4 | |||
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pLNCX1 Myr AKT2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27294 | protein kinase B beta | Homo sapiens | Ampicillin | Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | PH domain deleted (aa1-107) | Addgene | 2026-10-17 02:44:11 | 2 | ||
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pLNCX1 HA AKT2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27295 | protein kinase B beta | Homo sapiens | Ampicillin | There is a silent mutation at nt552 (CGG->CGA) of the depositor's seq. This mutation does not alter the amino acid sequence. | Backbone Size:6600; Vector Backbone:pLNCX; Vector Types:Retroviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:44:11 | 1 | ||
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fox1 (a2bp1l)_L (OZ519) Resource Report Resource Website |
RRID:Addgene_27194 | Zinc finger array targeting fox1 (a2bp1l) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene fox1 (a2bp1l) (see below). Please note that this plasmid does NOT contain the fox1 (a2bp1l) sequence. Users must order the complementary plasmid fox1 (a2bp1l)_R (OZ520) [Addgene plasmid 27195] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGTC-3' in a bacterial two hybrid assay, and resulted in 7.12 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:44:10 | 0 | |
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pitrm1_R (OZ516) Resource Report Resource Website |
RRID:Addgene_27191 | Zinc finger array targeting pitrm1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene pitrm1 (see below). Please note that this plasmid does NOT contain the pitrm1 sequence. Users must order the complementary plasmid pitrm1_L (OZ515) [Addgene plasmid 27190] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAAGAAGAG-3' in a bacterial two hybrid assay, and resulted in 8.11 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:44:10 | 0 | |
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pGEX6-Smad3 EPSM Resource Report Resource Website |
RRID:Addgene_27222 | Smad3 | Homo sapiens | Ampicillin | PMID:19917253 | Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-6P; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | T179V S204A S208A S213A | Addgene | 2026-10-17 02:44:10 | 0 | |
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GFP-TREX1(D18N) Resource Report Resource Website 1+ mentions |
RRID:Addgene_27220 | TREX1 | Homo sapiens | Kanamycin | PMID:20871604 | Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | D18N sequence 52-GAC changed to AAC, resulting in Aspartic acid to Asparagine change. | Addgene | 2026-10-17 02:44:10 | 7 | |
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LRP6-pCS2-VSVG Resource Report Resource Website 1+ mentions |
RRID:Addgene_27282 | low density lipoprotein receptor-related protein 6 | Homo sapiens | Ampicillin | PMID:14731402 | Contains full-length human LRP6. Please note that the VSVG-tag was inserted after the LRP6 signal peptide at amino acid 25. | Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:xenopus injection; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:44:11 | 4 | |
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pcDNA3/mPax3-3xHA Resource Report Resource Website 1+ mentions |
RRID:Addgene_27319 | Pax3 | Mus musculus | Ampicillin | PMID:7744814 | Backbone Size:5600; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:44:11 | 2 | ||
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pSport6/FLAG-Wwtr1(Taz) Resource Report Resource Website 1+ mentions |
RRID:Addgene_27318 | Taz | Mus musculus | Ampicillin | Backbone Size:5800; Vector Backbone:pEF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-17 02:44:11 | 2 | |||
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Integrin alpha5-AHCys Resource Report Resource Website |
RRID:Addgene_27313 | integrin alpha 5 | Homo sapiens | Ampicillin | PMID:11323715 | There is a minor discrepancy between BLAST truncation position and depositor's truncation position. The discrepancies could be the secretion peptide that the depositing lab does not include in their numbering, or the C-terminal tags (that's about 40 aa.) | Backbone Marker:Invitrogen; Backbone Size:6200; Vector Backbone:pEF1/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | integrin alpha5 (1-954) + AHCys | Addgene | 2026-10-17 02:44:11 | 0 |
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SEPT5 Mut(S327A) Resource Report Resource Website |
RRID:Addgene_27274 | Septin 5 | Homo sapiens | Ampicillin | PMID:18385322 | Backbone Marker:Clontech; Backbone Size:3791; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S327A | Addgene | 2026-10-17 02:44:11 | 0 | |
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SEPT5 Mut(S161A/S327A) Resource Report Resource Website |
RRID:Addgene_27275 | Septin 5 | Homo sapiens | Ampicillin | PMID:18385322 | Backbone Marker:Clontech; Backbone Size:3791; Vector Backbone:pCMV-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S161A and S327A | Addgene | 2026-10-17 02:44:11 | 0 | |
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pcDNA3.1 AKT3 HA (CT-gfp) Resource Report Resource Website 1+ mentions |
RRID:Addgene_27293 | protein kinase B gamma | Mus musculus | Ampicillin | The GFP is not expressed as there is a stop codon after the HA tag. mTORC2 reportedly can phosphorylate FSY and FSF sites, so the Y473F mutation should not affect expression or function. | Backbone Marker:Invitrogen; Backbone Size:6157; Vector Backbone:pcDNA3.1/CT-GFP-Topo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Y473F | Addgene | 2026-10-17 02:44:11 | 2 | |
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pEF1-alpha-V Resource Report Resource Website 1+ mentions |
RRID:Addgene_27290 | integrin alpha V | Homo sapiens | Ampicillin | PMID:12230977 | Backbone Marker:Invitrogen; Backbone Size:6200; Vector Backbone:pEF1/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Isoform 2 | Addgene | 2026-10-17 02:44:11 | 6 | |
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dpf2_R (OZ532) Resource Report Resource Website |
RRID:Addgene_27209 | Zinc finger array targeting dpf2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_L (OZ531) [Addgene plasmid 27208] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGAAACG-3' in a bacterial two hybrid assay, and resulted in 5.71 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:44:10 | 0 | |
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dpf2_L (OZ531) Resource Report Resource Website |
RRID:Addgene_27208 | Zinc finger array targeting dpf2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_R (OZ532) [Addgene plasmid 27209] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAAGTAGCA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:44:10 | 0 | |
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mdka (midkine-related growth factor)_R (OZ528) Resource Report Resource Website |
RRID:Addgene_27205 | Zinc finger array targeting mdka | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence. Users must order the complementary plasmid mdka (midkine-related growth factor)_L (OZ527) [Addgene plasmid 27204] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGGTGGC-3' in a bacterial two hybrid assay, and resulted in 3.75 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | Addgene | 2026-10-17 02:44:10 | 0 |
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