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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Authority Record Last Update Mentions Count
pGL4.11 promPDE4D3
 
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RRID:Addgene_27302 prom PDE4D3 Homo sapiens Ampicillin PMID:20952536 Backbone Size:4370; Vector Backbone:pGL4.11; Vector Types:Luciferase; Bacterial Resistance:Ampicillin N/A Addgene 2026-10-17 02:44:11 0
pcDNA3.2-hBIN1-V5
 
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RRID:Addgene_27303 bridging integrator 1 (BIN1), transcript variant 8 Homo sapiens Ampicillin PMID:20169111 Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-Dest (Invitrogen); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:11 0
pcDNA-RELΔ424-490
 
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RRID:Addgene_27267 REL Homo sapiens Ampicillin PMID:17072339 Please note that Addgene's sequencing results confirmed N424 is present in the REL sequence. Despite the plasmid name, amino acids 425-490 of REL have been deleted. This plasmid is exactly as created by the depositing laboratory and as described in the associated publication listed below and in Chin et al., Oncogene. 2009 May 21;28(20):2100-11. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Deletion of transactivation domain I (aa 425-490) Addgene 2026-10-17 02:44:11 0
pcDNA-FLAG-NEMO-C54/347A
 
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1+ mentions
RRID:Addgene_27268 NEMO Homo sapiens Ampicillin PMID:18164680 NEMO cDNA starts at aa 2. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Cys54Ala Cys347Ala Addgene 2026-10-17 02:44:11 2
pCDNA3 IKKe T7
 
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RRID:Addgene_27238 I-kappa-B kinase epsilon Homo sapiens Ampicillin PMID:12692549 Backbone Size:5400; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 0
pFSy(1.1)GW
 
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10+ mentions
RRID:Addgene_27232 EGFP Ampicillin PMID:15608064 For rodent cortex pyramidal neuron-specific expression of EGFP under the synapsin I promoter (1.1kb fragment) Backbone Size:9941; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 10
pFCK(1.3)tdimer2W
 
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1+ mentions
RRID:Addgene_27233 DsRed2 Ampicillin PMID:15608064 Expresses tandem dimer RFP under the mouse α-CaMKII promoter (1.3kb fragment). Similar to plasmid # 27230, except for fluorescent tag. Note that differences between Addgene's sequencing result and the sequence from the depositing scientist are due to use of theoretical maps. Please utilize Addgene's sequence when available. EcoRI and BstBI sites are not present according to Addgene's sequencing results. Backbone Size:9066; Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 1
hnf1a_R (OZ522)
 
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RRID:Addgene_27197 Zinc finger array targeting hnf1a Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene hnf1a (see below). Please note that this plasmid does NOT contain the hnf1a sequence. Users must order the complementary plasmid hnf1a_L (OZ521) [Addgene plasmid 27196] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGTAGTG-3' in a bacterial two hybrid assay, and resulted in 7.30 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:10 0
pFCK(1.3)GW
 
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1+ mentions
RRID:Addgene_27230 EGFP Ampicillin PMID:15608064 For rodent cortex pyramidal neuron-specific expression of EGFP under the mouse α-CaMKII promoter (1.3kb fragment) Backbone Size:9941; Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 3
VDAC2_L (OZ523)
 
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RRID:Addgene_27198 Zinc finger array targeting VDAC2 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene VDAC2 (see below). Please note that this plasmid does NOT contain the VDAC2 sequence. Users must order the complementary plasmid VDAC2_R (OZ524) [Addgene plasmid 27199] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGTTGGGAG-3' in a bacterial two hybrid assay, and resulted in 13.18 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:10 0
pFSy(336)GW
 
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1+ mentions
RRID:Addgene_27231 EGFP Ampicillin PMID:15608064 Expresses EGFP under the synapsin I promoter Backbone Size:9941; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 1
fox1 (a2bp1l)_R (OZ520)
 
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RRID:Addgene_27195 Zinc finger array targeting fox1 (a2bp1l) Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene fox1 (a2bp1l) (see below). Please note that this plasmid does NOT contain the fox1 (a2bp1l) sequence. Users must order the complementary plasmid fox1 (a2bp1l)_L (OZ519) [Addgene plasmid 27194] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGAGGGC-3' in a bacterial two hybrid assay, and resulted in 8.09 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:10 0
hnf1a_L (OZ521)
 
Resource Report
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RRID:Addgene_27196 Zinc finger array targeting hnf1a Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene hnf1a (see below). Please note that this plasmid does NOT contain the hnf1a sequence. Users must order the complementary plasmid hnf1a_R (OZ522) [Addgene plasmid 27197] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGGGAAGGT-3' in a bacterial two hybrid assay, and resulted in 9.60 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:10 0
pCLX-UBI-GFPf
 
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RRID:Addgene_27248 GFPf Aequorea Ampicillin Backbone Size:7879; Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:11 0
pCLX-UBI-GFP
 
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1+ mentions
RRID:Addgene_27245 GFP Aequorea Ampicillin PMID:23097121 Backbone Size:7860; Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 5
pCLX-UBI-Tred
 
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1+ mentions
RRID:Addgene_27246 TurboRFP Entacmaea Ampicillin PMID:29079819 Backbone Size:7932; Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 1
FLAG-TREX1
 
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1+ mentions
RRID:Addgene_27218 TREX1 Homo sapiens Ampicillin PMID:20871604 Backbone Size:5000; Vector Backbone:pFLAG-CMV4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin n/a Addgene 2026-10-17 02:44:10 3
GFP-TREX1
 
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1+ mentions
RRID:Addgene_27219 TREX1 Homo sapiens Kanamycin PMID:20871604 Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin n/a Addgene 2026-10-17 02:44:10 8
Kif6_L (OZ537)
 
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RRID:Addgene_27214 Zinc finger array targeting Kif6 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Kif6 (see below). Please note that this plasmid does NOT contain the Kif6 sequence. Users must order the complementary plasmid Kif6_R (OZ538) [Addgene plasmid 27215] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGAAGCT-3' in a bacterial two hybrid assay, and resulted in 10.27 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:10 0
Kif6_R (OZ538)
 
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RRID:Addgene_27215 Zinc finger array targeting Kif6 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Kif6 (see below). Please note that this plasmid does NOT contain the Kif6 sequence. Users must order the complementary plasmid Kif6_L (OZ537) [Addgene plasmid 27214] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGACTGC-3' in a bacterial two hybrid assay, and resulted in 5.06 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:10 0

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