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Species: Synthetic
Genetic Insert: Serum albumin followed by αMFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84970 Copy
Species: Synthetic
Genetic Insert: Enolase 1 promoter
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84962 Copy
Species: Synthetic
Genetic Insert: α-mating factor secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84964 Copy
Species: Synthetic
Genetic Insert: Triose phosphate isomerase 1 promoter
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84963 Copy
Species: Synthetic
Genetic Insert: α-mating factor no EAEA secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84965 Copy
Species: Synthetic
Genetic Insert: α-Amylase followed by MFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84968 Copy
Species: Synthetic
Genetic Insert: Glucoamylase followed by αMFΔ secretion tag
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84969 Copy
Species: Synthetic
Genetic Insert: Pichia autonomously replicating sequence
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84990 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MevTaco-MKco-PMKco
Vector Backbone Description: Vector Backbone:pBbA5c; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26052683
Proper citation: RRID:Addgene_84985 Copy
Species: Synthetic
Genetic Insert: Alcohol oxidase 1 terminator
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84988 Copy
Species: Synthetic
Genetic Insert: BxbI recognition site
Vector Backbone Description: Backbone Marker:Lee et al. (2015), ACS Synth. Biol. 4, 975–986.; Backbone Size:1662; Vector Backbone:pYTK001-EntryVector; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28252957
Comments: Design of this plasmid was performed according to the described Methods in Lee et al. 2015 (A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly) and this plasmid is compatible with the existing toolkit (MoClo-YTK, Kit number 1000000061)
Proper citation: RRID:Addgene_84989 Copy
Species: Synthetic
Genetic Insert: Pro-Pro-Pro-Pro-Pro
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2210; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27781345
Proper citation: RRID:Addgene_85098 Copy
Species: Bacillus megaterium
Genetic Insert: FADdomain of P450 BM3
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2227; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27781345
Comments: P450 BM3 was originally cloned from Bacillus megaterium (NBRC 15308) in our lab
Proper citation: RRID:Addgene_85104 Copy
Species: Synthetic
Genetic Insert: Putidaredoxin
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2210; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27781345
Comments: A synthetic gene of PdX was inserted between EcoRI and BamHI sites of pHSG398 using In-Fusion cloning kit.
Proper citation: RRID:Addgene_85100 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pJPC12; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27982027
Comments: The pJPC12 vector was obtained from Peterson and Phillips (Peterson, J. and G.J. Phillips, New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid, 2008. 59(3): p. 193-201)
Proper citation: RRID:Addgene_80859 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pJPC12; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27982027
Comments: The pJPC12 vector was obtained from Peterson and Phillips (Peterson, J. and G.J. Phillips, New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid, 2008. 59(3): p. 193-201)
Proper citation: RRID:Addgene_80914 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pJPC12; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27982027
Comments: The pJPC12 vector was obtained from Peterson and Phillips (Peterson, J. and G.J. Phillips, New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid, 2008. 59(3): p. 193-201)
Proper citation: RRID:Addgene_80913 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pJPC12; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27982027
Comments: The pJPC12 vector was obtained from Peterson and Phillips (Peterson, J. and G.J. Phillips, New pSC101-derivative cloning vectors with elevated copy numbers. Plasmid, 2008. 59(3): p. 193-201)
Proper citation: RRID:Addgene_80912 Copy
Species: Mus musculus
Genetic Insert: TET1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4008; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26932196
Proper citation: RRID:Addgene_81056 Copy
Species: E. coli, B. subtilis
Genetic Insert: Encodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: Tar[1-43]-McpC[49-270]-Tar[184-553]
Vector Backbone Description: Backbone Size:4211; Vector Backbone:pKG116; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27285081
Proper citation: RRID:Addgene_81227 Copy
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