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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Authority Record Last Update Mentions Count
pHR-mCherry-tSH2(ZAP70)
 
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1+ mentions
RRID:Addgene_27137 mCherry and ZAP70 (residues 1-259) Homo sapiens Ampicillin PMID:21135224 Backbone Size:9000; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 2
pHR-ZIP(RK)
 
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RRID:Addgene_27136 CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) Homo sapiens Ampicillin PMID:21135224 Backbone Size:9000; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Arginines in the SH2 domains mutated to lysines (R37K/R190K in human ZAP70; R718K/R871K in the insert) Addgene 2026-10-17 02:44:09 0
pHR-ZIP(WT)
 
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RRID:Addgene_27134 CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) Homo sapiens Ampicillin PMID:21135224 Backbone Size:9000; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 0
pEF5B-FRT-GFP-αTAT1
 
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10+ mentions
RRID:Addgene_27099 α-Tubulin K40 acetyltransferase1 Homo sapiens Ampicillin PMID:21068373 Backbone Marker:Invitrogen; Backbone Size:5971; Vector Backbone:pEF5B-FRT-LAP-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 16
pBiFC-VN155(I152L)
 
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10+ mentions
RRID:Addgene_27097 VN155(I152L) Aequorea victoria Ampicillin PMID:21091444 To be published by Biotechniques in December. Please suggest the citation of our Biotechniques article. Also, this will be used as a replacement of pBiFC-VN173. Backbone Marker:Clontech; Backbone Size:3791; Vector Backbone:pMyc-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin I152L Addgene 2026-10-17 02:44:09 32
p3xFLAG-CMV-Tlr4
 
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1+ mentions
RRID:Addgene_27148 toll-like receptor 4 Mus musculus Ampicillin PMID:9851930 Backbone Marker:Sigma; Backbone Size:4732; Vector Backbone:p3xFLAG-CMV-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Native signal peptide sequence (original amino acids 1-24) was removed for cloning. Addgene 2026-10-17 02:44:09 1
p3xFLAG-CMV-Tlr4(Lps)
 
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RRID:Addgene_27149 toll-like receptor 4(Lps) Mus musculus Ampicillin PMID:9851930 Backbone Marker:Sigma; Backbone Size:4732; Vector Backbone:p3xFLAG-CMV-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin changed Proline 712 to Histidine. Native signal peptide sequence (original amino acids 1-24) was removed for cloning. Addgene 2026-10-17 02:44:09 0
pDB1 (CK2alpha)
 
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1+ mentions
RRID:Addgene_27083 CK2alpha Homo sapiens Ampicillin PMID:21036246 Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4952; Vector Backbone:pGEX-3X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 7
pCXLE-EGFP
 
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50+ mentions
RRID:Addgene_27082 eGFP Ampicillin PMID:21460823 Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 62
pCXLE-hUL
 
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100+ mentions
RRID:Addgene_27080 L-MYC Homo sapiens Ampicillin PMID:21460823 Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 370
pSL-MS2-6X
 
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10+ mentions
RRID:Addgene_27118 MS2 binding sites (6) Ampicillin PMID:9809065 The Singer Lab no longer recommends this plasmid, as it has a higher rate of bacterial recombination. For mammalian cells they recommend the following plasmids: pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre (Plasmid #84561) HaloTag-bActinCDS-bActinUTR-MS2V5 (Plasmid #102718) For yeast cells they recommend the following plasmids: pET246-pUC57 12xMS2V6 (Plasmid #104390) pET259-pUC57 24xMS2V6 (Plasmid #104391) pET251-pUC 12xMS2V6 Loxp KANr Loxp (Plasmid #104392) pET264-pUC 24xMS2V6 Loxp KANr Loxp (Plasmid #104393) Loop sequence: acatgaggatcacccatgt Addgene's sequencing result contains 1 nucleotide mismatch at bp# 345 when compared to the full plasmid sequence provided by the depositing laboratory. Backbone Marker:Pharmacia; Backbone Size:3422; Vector Backbone:pSL1180; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 13
pCS2 Flag Smad3 S204A
 
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RRID:Addgene_27114 Smad3 Homo sapiens Ampicillin PMID:19917253 Backbone Size:4000; Vector Backbone:pCS2; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin S204A Addgene 2026-10-17 02:44:09 0
pCXLE-hSK
 
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100+ mentions
RRID:Addgene_27078 SOX2, KLF4 Homo sapiens Ampicillin PMID:21460823 Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 378
pCS2 Flag Smad3 EPSM A204S
 
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RRID:Addgene_27111 Smad3 Homo sapiens Ampicillin PMID:19917253 A204 was mutated back to the WT residue on the original pCS2-Flag Smad3 EPSM plasmid. Backbone Size:4000; Vector Backbone:pCS2; Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin T179V S204A S208A S213A; A204S Addgene 2026-10-17 02:44:09 0
pCXLE-hMLN
 
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10+ mentions
RRID:Addgene_27079 C-MYC, LIN28, NANOG Homo sapiens Ampicillin PMID:21460823 Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 11
pCXLE-hOCT3/4
 
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50+ mentions
RRID:Addgene_27076 OCT3/4 Homo sapiens Ampicillin PMID:21460823 Backbone Size:10180; Vector Backbone:pCXLE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 52
pGEX6-Smad3 EPSM A213S
 
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RRID:Addgene_27110 Smad3 Homo sapiens Ampicillin PMID:19917253 A213 was mutated back to the wild type residue in EPSM construct Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-6P; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin T179V S204A S208A S213A; A213S Addgene 2026-10-17 02:44:09 0
pGV13 (CK2alpha K68M, CK2beta)
 
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RRID:Addgene_27094 CK2beta Homo sapiens Ampicillin PMID:21036246 Backbone Marker:Clontech; Backbone Size:4358; Vector Backbone:pBI; Vector Types:Mammalian Expression, tetracycline-regulated expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 0
pGV7 (CK2alpha' K69M, CK2beta)
 
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RRID:Addgene_27095 CK2beta Homo sapiens Ampicillin PMID:21036246 Backbone Marker:Clontech; Backbone Size:4358; Vector Backbone:pBI; Vector Types:Mammalian Expression, tetracycline-regulated expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 0
pGP18 (CK2alpha' K69M)
 
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1+ mentions
RRID:Addgene_27090 CK2alpha' K69M Homo sapiens Ampicillin PMID:21036246 Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K69M Addgene 2026-10-17 02:44:09 1

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