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Species: Xenopus laevis
Genetic Insert: Dishevelled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: HA tagged Xenopus Dishevelled with a triple point mutation outside of the PDZ binding domain, that does not block binding to Dapper.
Notes for Use: Linearize with either Not1 or Asp718
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461
Proper citation: RRID:Addgene_16779 Copy
Species: Xenopus laevis
Genetic Insert: Dishevelled
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11970895
Comments: Description: HA tagged Xenopus Dishevelled with a point mutation in the binding pocket of the PDZ binding domain, that blocks binding to Dapper.
Notes for Use: Linearize with either Not1 or Asp718.
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461.
Proper citation: RRID:Addgene_16780 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Transcription: linearize with Not I and transcribe with SP6.
Plasmid History:
Constructed by Randall T. Moon.
Notes for Use:
Xdsh of S. Sokol with GFP of Heim et al., Nature 1995, cloned at the carboxy terminal end, followed by a translation stop codon.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.
Addgene QC found 2 point mutations, T511A and T717S, that do not affect function.
Proper citation: RRID:Addgene_16788 Copy
Species: Xenopus laevis
Genetic Insert: Xdsh-delta-COOH
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10427095
Comments: GFP-tagged XDsh of Yang-Snyder et al. (Curr. Biology, 1996) was engineered to now contain Dsh amino acids 1-588 (deleting 599-736).
Linearize with Not1 and transcribe with SP6.
Ref: J.R. Miller et al., (1999), J. Cell Biology 146:427-437.
Proper citation: RRID:Addgene_16784 Copy
Species: Xenopus laevis
Genetic Insert: TM beta-catenin 1-myc
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314542
Comments: TM of N-cadherin to NH2 terminal deletion of Beta-catenin myc.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
Proper citation: RRID:Addgene_16834 Copy
Species: Xenopus laevis
Genetic Insert: beta-catenin-myc
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8666229
Comments: Plasmid History:
Constructed by David on date of 2/9/95 in book XVI p.106.
Notes for Use:
Full-length Xenopus beta-catenin reconstructed by taking the Cla-SphI vector fragment from XBC20 and the SphI-Cla sequences from XBC24 (encoding the C-terminus of beta-catenin). This corrects possible problems with the C-terminus os XBC20. To make RNA, cut with NotI and transcribe with SP6.
Ref: Yost et al. Genes and Development 10:1443-1454, 1996.
Proper citation: RRID:Addgene_16842 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-8 RNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1879349
Comments: Insert: EcoR1 insert blunted into Bgl II blunt of SP64T.
Transcription: linearize with Bam H1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian on date of 12/90.
Citation: Christian et al., Development 111, 1045 (1991)
Proper citation: RRID:Addgene_16869 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-8
Vector Backbone Description: Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:CS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: EcoR1 insert from pGEM Xwnt-8 subcloned into EcoR1 of CS2+;
Sense RNA cut with ASP718 and transcribe with SP6.
Constructed by Jeff Brown, unpublished.
Proper citation: RRID:Addgene_16865 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-11
Vector Backbone Description: Backbone Size:0; Vector Backbone:pT7TS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7739543
Comments: Insert: full length coding seq only, by PCR of lambda clones isolated by Purcell.
Insert size: 1 Kb.
Transciption: linearize with Sal1, Xba1, or BamH1 and transcribe with T7.
Plasmid History:
Constructed by Susan Purcell on date of 5/94.
Citation: Du et al., Molec. Cell Bio., 15, 2625 (1995)
Ku and Melton, Development, 119, 1161 (1993)
Proper citation: RRID:Addgene_16861 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-4 myc
Vector Backbone Description: Backbone Size:2999; Vector Backbone:SP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: See clone XE2 for details. A single c-myc epitope recognized by the monoclonal antibody 9E10 has been addes to the C-terminus.
Transcription: linearize with Sal1 and transcribe with SP6.
Citation: L. Lynn McGrew, unpublished.
Proper citation: RRID:Addgene_16877 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-4myc
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: See clone XE2 for details and XE62 for myc tag, and V4 for vector details.
Notes for Use:
Citation: L. Lynn McGrew, unpublished.
Proper citation: RRID:Addgene_16876 Copy
Species: Xenopus laevis
Genetic Insert: full coding of Xwnt-5A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Insert: full coding of Xwnt-5A.
Insert size: 1.2 Kb.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by RTM/JR on the date of 4/93.
Notes for Use:
This construct encodes full coding sequence of Xwnt-5A, without wnt UTR's.
See Xwnt 5A full-length for Genbank data.
Proper citation: RRID:Addgene_16874 Copy
Species: Xenopus laevis
Genetic Insert: Wnt-5A
Vector Backbone Description: Backbone Marker:Available from Addgene (#17120); Backbone Size:0; Vector Backbone:pCD-betaG-PS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The myc tagged Xwnt-5A insert of clone XE40, in the SV40 construct pCD-PS modified by Julia Yang-Snyder to include Xenopus beta globin flanking untranslated regions.
Reference: The clone was made by Julia Yang-Snyder.
Addgene QC found a G125A point mutation that is not known to affect function.
Proper citation: RRID:Addgene_16873 Copy
Species: Xenopus laevis
Genetic Insert: Engrailed-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Xenopus Probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1679005
Comments: In situ probe cut with Xba1, transcribe with T3.
Proper citation: RRID:Addgene_16950 Copy
Species: Xenopus laevis
Genetic Insert: Xwnt-5A
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8275867
Comments: Insert: cDNA from Melton lambda gt10 oocyte library.
Transcription: linearize with Xba1 and transcribe with SP6.
Plasmid History:
Constructed by Randall Moon on date of 9/15/90.
Notes for Use:
EcoR1 insert of lambda G6 of 2/11/90. Contains significant 5' UTR and is less translatable in vitro per ng RNA than Xwnt-5A Hind III/SP64T. However, the type of activity is the same in both constructs.
Citation: Moon et al., Development 119, 97 (1993)
Proper citation: RRID:Addgene_16985 Copy
Species: Xenopus laevis
Genetic Insert: banded hedgehog
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Xenopus Hedgehog. Antisense are generated as listed:
Xenopus banded hedgehog: Linearize clone XIL in Bluescript KS with BamH1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus sonic hedgehog: Linearize clone X32 in Bluescript KS with Bam H1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus cephalic hedgehog:Linearize clone X30 in pT7TS with Sal 1 and transcribe with T7.
Proper citation: RRID:Addgene_16984 Copy
Species: Xenopus laevis
Genetic Insert: Goosecoid
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: coding region of Xenopus goosecoid in SP64T in sense (#9) & antisense (#2) orientation.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian by PCR.
Goosecoid cloned and characterized by lab of E. DeRobertis, UCLA.
Proper citation: RRID:Addgene_16979 Copy
Species: Xenopus laevis
Genetic Insert: Xbra
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:psp73; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: full length Xbra from Jim Smith.
Antisense: linearize with EcoRV, transcribe with T7.
Plasmid History:
Constructed by Jan Christian.
Proper citation: RRID:Addgene_16978 Copy
Species: Xenopus laevis
Genetic Insert: noggin PCR clone
Vector Backbone Description: Backbone Size:4120; Vector Backbone:pCSKA; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Insert: noggin PCR clone (coding region). In reverse orientation compared to CSKA promoter.
Plasmid History:
Constructed by Jan Christian.
Isolated and characterize by W. Smith and R. Harland.
Proper citation: RRID:Addgene_16998 Copy
Species: Xenopus laevis
Genetic Insert: noggin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Insert: noggin PCR clone (coding region) in SP64T.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by Jan Christian.
Noggin isolated and chacterized by W. Smith and R. Harland.
Proper citation: RRID:Addgene_16997 Copy
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