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Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170202 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170203 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 S (spike) B.1.429
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170200 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 S (spike) D614G
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170201 Copy
Species: SARS-CoV-2
Genetic Insert: Nucleocapsid
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.10.459410v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_170466 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike
Vector Backbone Description: Backbone Size:4971; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_170389 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike protein
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:2900; Vector Backbone:pRSET5A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_171709 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Receptor Binding domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5442; Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167126 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp3 (residues 1584-1945)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167261 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp3 (residues 1089-1203)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167260 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp15 (residues 1-346)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167265 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp9 (residues 1-113)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167264 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp3 (residues 413-676)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167258 Copy
Species: SARS-CoV-2
Genetic Insert: Nsp1 (residues 13-127)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167256 Copy
Species: SARS-CoV-2
Genetic Insert: N protein (residues 1-419)
Vector Backbone Description: Vector Backbone:pMCSG53; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_167255 Copy
Species: SARS-CoV-2
Genetic Insert: nsp10, nsp16
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: SGC clone sample ID: nsp16_SARS2-nsp10_SARS2-MVC019-A07:C245477
Proper citation: RRID:Addgene_167849 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike
Vector Backbone Description: Backbone Marker:Vectorbuilder; Vector Backbone:pRP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Codon-optimized SARS-CoV-2 Spike was cloned from SinoBiological plasmid VG40589-UT. An 18-amino acid truncation was introduced to improve expression. The plasmid can be used to generate Spike-pseudotyped lentiviral or VSV particles.
Proper citation: RRID:Addgene_164583 Copy
Species: SARS-COV-2
Genetic Insert: Nsp3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165108 Copy
Species: SARS-CoV-2
Genetic Insert: nsp10
Vector Backbone Description: Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Unlike SARS-CoV-2 nsp10 that gets expressed in the context of a larger polypeptide, this SARS-CoV-2 nsp10 gets expressed from a 5' ATG, and thus might contain a methionine at the N-terminus that is not present in the nsp5-cleaved nsp10 in cells.
Proper citation: RRID:Addgene_169158 Copy
Species: SARS-CoV-2
Genetic Insert: 3xFlag-nsp5CS[VATLQ]-nsp14
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: We have included the predicted cleavage site for nsp5 found between nsp13 and nsp14 (VATLQ) between the 3xFlag tag and nsp14 in this construct, which should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
Proper citation: RRID:Addgene_169159 Copy
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