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Species: Escherichia coli
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30122535
Proper citation: RRID:Addgene_113122 Copy
Species: Escherichia coli
Genetic Insert: Rho
Vector Backbone Description: Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30122535
Proper citation: RRID:Addgene_113121 Copy
Species: Escherichia coli
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30122535
Proper citation: RRID:Addgene_113124 Copy
Species: Escherichia coli
Genetic Insert: Acetyl-CoA synthetase (ACS)
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30085399
Comments: Wild Type Gene
Proper citation: RRID:Addgene_108943 Copy
Species: Escherichia coli
Genetic Insert: FtnA
Vector Backbone Description: Vector Backbone:plasmid PAtagRFP-actin (Verkhusha lab/Addgene); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29500346
Proper citation: RRID:Addgene_108969 Copy
Species: Escherichia coli
Genetic Insert: ClpX
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29107333
Comments: Depositor confirms K408E compared to NC_000913.3 has no impact on function.
Proper citation: RRID:Addgene_111514 Copy
Species: Escherichia coli
Genetic Insert: CRISPR spacers targeting IS1, IS5, IS3
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pCRISPathBrick; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31008359
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/428029 for BioRxiv preprint.
Proper citation: RRID:Addgene_120425 Copy
Species: Escherichia coli
Genetic Insert: CRISPR spacers targeting IS1, IS5, IS3, IS150
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pCRISPathBrick; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31008359
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/428029 for BioRxiv preprint.
Proper citation: RRID:Addgene_120424 Copy
Species: Escherichia coli
Genetic Insert: BetI repressor
Vector Backbone Description: Vector Backbone:pEXT20; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: Plasmid also contains YFP reporter (whose expression is controlled by a constitutive promoter containing an operator site for the indicated repressor).
Proper citation: RRID:Addgene_49357 Copy
Species: Escherichia coli
Genetic Insert: AcrR
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51148 Copy
Species: Escherichia coli
Genetic Insert: BetI
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51156 Copy
Species: Escherichia coli
Genetic Insert: mutL E32K
Vector Backbone Description: Backbone Marker:Datta S. et al. (Gene 379 (2006) 109-115); Backbone Size:6248; Vector Backbone:pSIM8; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26884157
Comments: Please visit the depositor's website: http://group.szbk.u-szeged.hu/sysbiol/pal-csaba-lab-resources.html for additional resource information.
Proper citation: RRID:Addgene_72680 Copy
Species: Escherichia coli
Genetic Insert: ORF17_DHNTPase_NUD
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17698004
Proper citation: RRID:Addgene_73154 Copy
Species: Escherichia coli
Genetic Insert: guanylate kinase
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18707057
Comments: Source was E. coli K-12 MRE6000
Proper citation: RRID:Addgene_73631 Copy
Species: Escherichia coli
Genetic Insert: formylglycinamidine ribonucleotide synthetase
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Repaired form of a plasmid described originally in Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR (2008) Pathway engineered enzymatic de novo purine nucleotide synthesis. ACS Chem Biol. 3, 499-511. doi: 10.1021/cb800066p
Proper citation: RRID:Addgene_74277 Copy
Species: Escherichia coli
Genetic Insert: phosphoribosylglycinamide synthetase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Constructed by Danny Crawford and T. J. Kappock in the laboratory of JoAnne Stubbe (MIT).
In JM109 strain.
Proper citation: RRID:Addgene_75077 Copy
Species: Escherichia coli
Genetic Insert: rpsA
Vector Backbone Description: Backbone Size:4520; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31832686
Proper citation: RRID:Addgene_83585 Copy
Species: Escherichia coli
Genetic Insert: NptII
Vector Backbone Description: Backbone Marker:self-made; derived from pCambia1302 generated at the Cambia Institute; Backbone Size:6698; Vector Backbone:pDGB3omega1R; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26839579
Proper citation: RRID:Addgene_75471 Copy
Species: Escherichia coli
Genetic Insert: Release factor 2
Vector Backbone Description: Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124130 Copy
Species: Escherichia coli
Genetic Insert: Ribosome recycling factor
Vector Backbone Description: Vector Backbone:pQE60; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11479568
Comments: It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
Proper citation: RRID:Addgene_124132 Copy
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