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Species:
Genetic Insert: pmyc1tetO-gfp, tetR(B)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
References:
Comments: Expresses GFP under control of tetR(B). Also see Pubmed IDs 18059281 and 15687379.
pUV15 derivative, Genotype: Hygr, Kmr, pimyc-tetR, pmyc1 tetO-gfp+
Proper citation: RRID:Addgene_17975 Copy
Species: Synthetic
Genetic Insert: mEos3.2_H-NSdbd
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_168774 Copy
Species: Synthetic
Genetic Insert: mEos3.2
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_168775 Copy
Species:
Genetic Insert: pKW08-Lx-Int
Vector Backbone Description: Backbone Size:8535; Vector Backbone:pMC1M; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_25015 Copy
Species: Synthetic gene
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-rpsA; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_24663 Copy
Species: Synthetic gene
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_24664 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli.
This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.
References: this paper has been accepted in RSC Advances:
A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam
Proper citation: RRID:Addgene_117695 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AC; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments: Contains 34 bp GoldenMOCS-Yali Linker AB
Proper citation: RRID:Addgene_117828 Copy
Species: A. niger
Genetic Insert: pTEF1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_118076 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBSV2H; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Addgene QC identified a partial duplication of the segment of the plasmid containing the HygR selectable marker. The plasmid is functional for transformation and selection in B. burgdorferi. This plasmid was functional for use as described in Takacs et al. 2018.
Proper citation: RRID:Addgene_118229 Copy
Species: Synthetic
Genetic Insert: Fab_Quin-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_119049 Copy
Species: Synthetic
Genetic Insert: Fab_MTX-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_119047 Copy
Species:
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_119885 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pML2424; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin
References:
Comments: Addgene QC Next-generation sequencing identified sequence discrepancies relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_117497 Copy
Species:
Genetic Insert: multiple cloning site
Vector Backbone Description: Backbone Size:5059; Vector Backbone:pMS2; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_20320 Copy
Species: synthetic gene derived from Tn10 tetR
Genetic Insert: Pimyc-tetR#10
Vector Backbone Description: Backbone Size:5133; Vector Backbone:pTE-mcs; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments: There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
Proper citation: RRID:Addgene_20318 Copy
Species:
Genetic Insert: I-SceI
Vector Backbone Description: Vector Backbone:pmsg383; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_48789 Copy
Species: Other
Genetic Insert: T38M-P750-luxAB-DAS+4
Vector Backbone Description: Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_49520 Copy
Species: Other
Genetic Insert: P750-luxAB-DAS+4
Vector Backbone Description: Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_49519 Copy
Species: Homo sapiens
Genetic Insert: grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_50365 Copy
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