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Genetic Insert: fabI-gltA1-gltA2-udhA-zwf-gapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87149 Copy
Genetic Insert: gapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87146 Copy
Species: Bacillus subtilis
Genetic Insert: XynA signal peptide
Vector Backbone Description: Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28230977
Proper citation: RRID:Addgene_87372 Copy
Genetic Insert: SpyTag
Vector Backbone Description: Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28230977
Proper citation: RRID:Addgene_87375 Copy
Genetic Insert: mSpyCatcher
Vector Backbone Description: Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28230977
Proper citation: RRID:Addgene_87374 Copy
Species: Groundwater metagenome
Genetic Insert: CasY1
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28005056
Proper citation: RRID:Addgene_87687 Copy
Species: Deltaproteobacteria
Genetic Insert: CasX1
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28005056
Proper citation: RRID:Addgene_87685 Copy
Species: B. zoohelcum ATCC 43767
Genetic Insert: Cas13b
Vector Backbone Description: Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28065598
Comments: Benchling sequence link: https://benchling.com/s/seq-gyNy5vRceFfoBk3kqStl
Proper citation: RRID:Addgene_89901 Copy
Species: P. buccae ATCC 33574
Genetic Insert: Cas13b
Vector Backbone Description: Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28065598
Comments: Benchling sequence link: https://benchling.com/s/hqikFD6s
Proper citation: RRID:Addgene_89906 Copy
Species: S. pyogenes
Genetic Insert: Cas9HF1
Vector Backbone Description: Vector Backbone:pCas9-CR4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28915012
Comments: This Cas9 has been modified to contain the high-fidelity mutations for SpCas9-HF1 from Kleinstiver et al 2016
Proper citation: RRID:Addgene_89961 Copy
Species: S. pyogenes
Genetic Insert: Cas9HF1
Vector Backbone Description: Vector Backbone:pEM-Cas9HF1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28915012
Proper citation: RRID:Addgene_89962 Copy
Vector Backbone Description: Backbone Marker:June Scott (PMID 12867436); Backbone Size:4000; Vector Backbone:pSH71 (pJRS1315); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18667560
Comments: Contains MCS-B (from pASK43+). The backbone of this vector is based on pJRS1315-Dr. June Scott (Emory University) and pOri23-Dr. Philippe Moreillon (Centre Hospitalier Universitaire Vaudois) with additional components from pEU308- Dr. June Scott (Emory University).
This plasmid can also be used for His-tag or Strep-tag fusion protein expression in gram-positive bacteria.
Proper citation: RRID:Addgene_90190 Copy
Vector Backbone Description: Backbone Marker:June Scott (PMID 12867436); Backbone Size:4000; Vector Backbone:pSH71 (pJRS1315); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18667560
Comments: Contains MCS-A (Synthetic). The backbone of this vector is based on pJRS1315-Dr. June Scott (Emory University) with additional components from pAL2-Dr. Vyv Salisbury (University of the West of England).
Proper citation: RRID:Addgene_90187 Copy
Vector Backbone Description: Backbone Marker:June Scott (PMID 12867436); Backbone Size:4000; Vector Backbone:pSH71 (pJRS1315); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18667560
Comments: Contains MCS-B (from pASK43+). The backbone of this vector is based on pJRS1315-Dr. June Scott (Emory University) and pOri23- Dr. Philippe Moreillon (Centre Hospitalier Universitaire Vaudois).
This plasmid can also be used for His-tag or Strep-tag fusion protein expression in gram-positive bacteria.
Proper citation: RRID:Addgene_90188 Copy
Species: Shewanella halifaxensis HAW-EB4
Genetic Insert: Thiosulfate response regulator
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28373240
Proper citation: RRID:Addgene_90953 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91229 Copy
Species: E.coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91226 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91227 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91244 Copy
Species: E. coli
Genetic Insert: folA
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31189117
Proper citation: RRID:Addgene_91242 Copy
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