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Genetic Insert: 18xHis tag
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pFA6akanMX6; Vector Types:PCR tagging yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16823883
Proper citation: RRID:Addgene_26883 Copy
Genetic Insert: 18xHis tag
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pFA6akanMX6; Vector Types:PCR tagging yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16823883
Proper citation: RRID:Addgene_26882 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-LIN28A-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20888316
Comments: Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26819 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-OCT4-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20888316
Comments: Note that OCT4 has some silent mutations.
Also note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26816 Copy
Species: Caenorhabditis elegans
Genetic Insert: dcap-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Proper citation: RRID:Addgene_26938 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5883; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26812 Copy
Species: Xenopus laevis
Genetic Insert: Tektin 2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18552847
Comments: Addgene's sequencing results identified a I429A mutation in the Tektin 2 sequence when compared to GenBank reference sequence NP_001089680.1
Proper citation: RRID:Addgene_26932 Copy
Species: Caenorhabditis elegans
Genetic Insert: lag-2 promoter
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid #1497); Backbone Size:4492; Vector Backbone:pPD95.81; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7568229
Comments: Quick confirmation: BamHI should result in two fragments: ~3kb and 4.5kb.
This is a lag-2 promoter::GFP fusion. Strong GFP in live worms. 3kb BamHI fragment of pJK520 is the complete 3kb 5' region of lag-2.
Proper citation: RRID:Addgene_26814 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5260; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26813 Copy
Species: Homo sapiens
Genetic Insert: dystrophin
Vector Backbone Description: Backbone Size:6656; Vector Backbone:pRRL-cPPT-X-PRE-SIN; Vector Types:Lentiviral, lentiviral plasmid backbone from L. Naldini; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18511457
Comments: Human (alpha) Skeletal Actin (HSA) promotor
Vp intron
Proper citation: RRID:Addgene_26810 Copy
Genetic Insert: HBTH tag N-terminal
Vector Backbone Description: Backbone Size:4150; Vector Backbone:pFA6a-TRP-PGAL1; Vector Types:PCR tagging yeast; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_26898 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:E.coli bacterial strain; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19633652
Comments: MG1655, bla, bio-, lambda-Red1
From the paper: Lambda prophage (from strain DY330; Yu et al. (2000), PNAS 97: 5978-5983) was modified to include the bla gene and introduced into wild-type MG1655 E.coli by P1 transduction at the bioA/bioB gene locus and selected on ampicillin.
Proper citation: RRID:Addgene_26930 Copy
Species: Mus musculus
Genetic Insert: NICD
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16508304
Proper citation: RRID:Addgene_26891 Copy
Species: Homo sapiens
Genetic Insert: NICD3
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCLE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16508304
Comments: EF1a xenopus enhancer-promoter-hNICD3-IRES-AlkPhos
A1757T mutation in Notch3 is not known to affect function.
Proper citation: RRID:Addgene_26894 Copy
Species: jellyfish
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:11406; Vector Backbone:R26/P; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15306687
Proper citation: RRID:Addgene_26890 Copy
Species: Mycobacteriophage
Genetic Insert: Che9c gene 61
Vector Backbone Description: Backbone Size:6758; Vector Backbone:pLAM12; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17179933
Proper citation: RRID:Addgene_26903 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HBT-tagged ubiquitin
Vector Backbone Description: Backbone Size:5891; Vector Backbone:YEplac195-CUP1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16432255
Proper citation: RRID:Addgene_26900 Copy
Species: Mus musculus
Genetic Insert: Hes5 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:0; Vector Backbone:PGV-B; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17721509
Comments: Contains the Hes5 promoter (-685 to +28).
C->T mutation in the center of the promoter does affect function.
Proper citation: RRID:Addgene_26869 Copy
Species: Mus musculus
Genetic Insert: Hes5 promoter
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4200; Vector Backbone:pDsRed2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17721509
Comments: Hes5p from -685 to +28.
Proper citation: RRID:Addgene_26868 Copy
Genetic Insert: eGFP-CAAX-PTVI-2A-mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5840; Vector Backbone:pEF6V5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17941043
Comments: This is a single ORF (AgeI) Kozak-eGFP-CAAX-PTVI-2A-mCherry Sopt (ClaI).
AgeI and ClaI were added by PCR when generating the tg and those sites are not noted in the MCS of EF6V5.
Made as TOPO TA PCR cloning. Use SpeI/XbaI to validate insert in vector.
Proper citation: RRID:Addgene_26901 Copy
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