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Species: Synthetic
Genetic Insert: Bacterial SP crRNA to prototspacer A
Vector Backbone Description: Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24076762
Proper citation: RRID:Addgene_48649 Copy
Genetic Insert: aiiA
Vector Backbone Description: Vector Backbone:pZ vector; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22178928
Proper citation: RRID:Addgene_48888 Copy
Species: Shewanella amazonensis SB2B
Genetic Insert: AS02_915
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49729 Copy
Species: E. coli
Genetic Insert: ACP transacylase
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pACM4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23361000
Proper citation: RRID:Addgene_49809 Copy
Species: Burkholderia thailandensis E264
Genetic Insert: AS37_2513
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49751 Copy
Species: Pseudomonas aeruginosa PAO1
Genetic Insert: AS35_3582
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49750 Copy
Species: Streptomyces coelicolor A3(2)
Genetic Insert: AS27_4265
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49745 Copy
Species: Bacillus subtilis subsp. subtilis str. 168
Genetic Insert: AS30_35
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49746 Copy
Species: Xanthomonas axonopodis pv. citri str. 306
Genetic Insert: AS22_1147
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49740 Copy
Species: Pseudomonas fluorescens Pf-5
Genetic Insert: AS20_992
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49739 Copy
Species: Vibrio parahaemolyticus RIMD 2210633
Genetic Insert: AS11_987
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49733 Copy
Species: Chlorobium tepidum TLS
Genetic Insert: AS04_1609
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49732 Copy
Species: Porphyromonas gingivalis W83
Genetic Insert: AS03_1244
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49731 Copy
Species: Bacteroides thetaiotaomicron VPI-5482
Genetic Insert: AS03_1198
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49730 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pBbS5c; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25496359
Comments: This plasmid carries G179V in Maqu_3494 compared to the genomic reference sequence. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_86205 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pBbS5c; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25496359
Proper citation: RRID:Addgene_86203 Copy
Species: Synthetic
Genetic Insert: Pveg-sLucopt
Vector Backbone Description: Backbone Marker:Robert Fagan and Neil Fairweather; Backbone Size:6378; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please note that the individual components of this construct (that is, Pveg and sLucopt) are described in Oliveira Paiva AM et al (2016) ACS Synth Biol (DOI: 10.1021/acssynbio.6b00104). During the cloning a mutation occurred that removes the SacI site in between the Pveg and sLucopt genes. The 5' (KpnI) and 3' (BamHI) restriction sites remain.
Proper citation: RRID:Addgene_86554 Copy
Vector Backbone Description: Backbone Size:5328; Vector Backbone:pBBR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28931397
Proper citation: RRID:Addgene_86709 Copy
Genetic Insert: gltA1-gltA2-udhA-GapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87151 Copy
Genetic Insert: gltA1-gltA2-gapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87150 Copy
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