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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
AEB-Theo45
 
Resource Report
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1+ mentions
RRID:Addgene_63850 Theo45 theophylline riboswitch Synthetic Chloramphenicol PMID:26621913 Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-29 01:08:37 2
pAraH6HATT
 
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RRID:Addgene_63901 TT Fab Homo sapiens Chloramphenicol Provided with the pComb3H system for arabinose induced expression of soluble protein with detection tags. The “TT” refers to the human Fab to tetanus toxin which can be used as a protein expression control. Alternatively, one could upgrade to the pComb3X system, also available on Addgene, which already contains tags. Select reference: Cary, S.P.; Lee, J.; Wagenknecht, R.; Silverman, G.J. (2000) Characterization of Superantigen-Induced Clonal Deletion with a Novel Clan III-Restricted Avian Monoclonal Antibody: Exploiting Evolutionary Distance to Create Antibodies Specific for a Conserved VH Region Surface. J. Immun., 164, 4730-4741. Backbone Size:3500; Vector Backbone:pAra; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-29 01:08:43 0
pEERM4 Cm
 
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1+ mentions
RRID:Addgene_64026 Chloramphenicol PMID:26133196 Backbone Marker:DNA 2.0; Backbone Size:3792; Vector Backbone:pJ344; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-29 01:08:45 1
pBL4
 
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RRID:Addgene_50548 CcaS Synechocystis sp. 6803 Chloramphenicol PMID:24608181 Note that Addgene NGS identified a nonsense mutation in the CcaS insert Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 0
pCph8
 
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RRID:Addgene_50552 Cph8 Synthetic Chloramphenicol PMID:24608181 Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter. Requires pPLPCB(S)t or some other source of TetR Requires PCB to function Does NOT include the response regulator. Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 0
pJT119b
 
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1+ mentions
RRID:Addgene_50551 CcaS Synechocystis sp. 6803 Chloramphenicol PMID:24608181 Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 4
pCBC-MT1T2
 
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1+ mentions
RRID:Addgene_50593 T1, gRNA scaffold, OsU3t plus, TaU3p, T2 Synthetic Chloramphenicol PMID:25432517 Please note that this plasmid runs as a dimer (>7kb) or multimer. Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 1
pCBC-MT3T4
 
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1+ mentions
RRID:Addgene_50595 T3, gRNA scaffold, U6-26t plus, OsU3p, T4 Synthetic Chloramphenicol PMID:25432517 Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 1
pCBC-DT1T2
 
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1+ mentions
RRID:Addgene_50590 T1, U626t plus, U629p, T2 Synthetic Chloramphenicol PMID:25432517 Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 8
pCBC-DT3T4
 
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1+ mentions
RRID:Addgene_50592 T3, gRNA scaffold, U6-1tplus, U6-26p, T4 Synthetic Chloramphenicol PMID:25432517 Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 3
His10-tag
 
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RRID:Addgene_55179 His10 Synthetic Chloramphenicol PMID:24295448 For sequencing of insert, use the following primers: fwd: TGCCACCTGACGTCTAAG rev: ATTACCGCCTTTGAGTGA Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol 2026-08-29 01:07:17 0
cMyc-tag
 
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RRID:Addgene_55183 cMyc tag Synthetic Chloramphenicol PMID:24295448 For sequencing of insert, use the following primers: fwd: TGCCACCTGACGTCTAAG rev: ATTACCGCCTTTGAGTGA Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol 2026-08-29 01:07:17 0
ML40K1
 
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RRID:Addgene_61920 none Chloramphenicol PMID:21925267 Genotype = cyo ilvE avtA aspC hisG argH metA lysA Precursor strain = ML31 Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys ML40(K1) contains an extra pACYC plasmid carrying the cat cassette plus extra tRNA genes (argU, ileY, leuW) from Agilent Technologies Inc. *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain. Vector Backbone:none; Vector Types:; Bacterial Resistance:Chloramphenicol 2026-08-29 01:08:19 0
pMU102
 
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RRID:Addgene_61805 Chloramphenicol PMID:22578246 Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-29 01:08:20 0
pACYC-GST
 
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1+ mentions
RRID:Addgene_62329 Chloramphenicol Backbone Marker:Structural Genomics Consortium; Backbone Size:8362; Vector Backbone:pACYC-LIC+; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-29 01:08:26 1
pCas9-CR4
 
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10+ mentions
RRID:Addgene_62655 Cas9 nuclease Chloramphenicol PMID:26463009 Please note that this plasmid runs as a dimer (>13kb). While this may reduce DNA yield, the plasmid still functions as expected. Vector Backbone:pdCas9-bacteria; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-29 01:08:26 23
pAK501
 
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RRID:Addgene_48107 Chloramphenicol PMID:23995928 Backbone Marker:Dieter Haas; Backbone Size:8270; Vector Backbone:pME6010; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:13 0
PM-TD!TB
 
Resource Report
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RRID:Addgene_48656 Bacterial TD crRNA to prototspacer B Synthetic Chloramphenicol PMID:24076762 Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:18 0
PM-TD!TA
 
Resource Report
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RRID:Addgene_48655 Bacterial TD crRNA to prototspacer A Synthetic Chloramphenicol PMID:24076762 Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:18 0
PM-ST1!TB
 
Resource Report
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RRID:Addgene_48654 Bacterial ST1 crRNA to prototspacer B Synthetic Chloramphenicol PMID:24076762 Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:18 0

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